LOCUS       sequence-408534-        9843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mutant JAK3 (M511I) that is able to drive constitutive
            activation of the JAK/STAT pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Omari SA, Kosasih HJ, Chung T, de Bock CE
  TITLE     In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in 
            leukemia.
  JOURNAL   Heliyon. 2023 Nov 8;9(11):e22085. doi: 
            10.1016/j.heliyon.2023.e22085. eCollection 2023 Nov.
  PUBMED    38053908
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Heliyon."; 
            date: "2023-11-8"; volume: "9(11):e22085. doi"; pages: " 
            10.1016/j.heliyon.2023.e22085. eCollection 2023 Nov"
COMMENT     Sequence Label: MSCV-JAK3(M511)_IRES_GFP
FEATURES             Location/Qualifiers
     source          1..9843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..158
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             358..874
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    938..1279
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1346..1762
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1692..1714
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1730..1746
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5193..5760
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5210..5762
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5354..5371)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5581..5600
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5774..5776,5777..5779,5780..6493)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5819..5840)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6080..6099)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6427..6448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6595..7109
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7271..7287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7295..7301,7302..7319,7320..7325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7340..7361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7478..7495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7649..8237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7729..7748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8408..9199,9200..9268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9031..9050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9269..9373)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9441..9459
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9497..9519)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9619..9638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(9832..9843,1..11)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttgtaaaac gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc
       61 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
      121 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
      181 gccggtgatg ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga
      241 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
      301 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat
      361 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
      421 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
      481 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
      541 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
      601 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
      661 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
      721 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgta ttcccaataa
      781 agcctcttgc tgtttgcatc cgaatcgtgg actcgctgat ccttgggagg gtctcctcag
      841 attgattgac tgcccacctc gggggtcttt catttggagg ttccaccgag atttggagac
      901 ccctgcccag ggaccaccga cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt
      961 gtctgtctct gtctttgtgc gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta
     1021 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     1081 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     1141 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     1201 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     1261 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     1321 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     1381 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     1441 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     1501 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     1561 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     1621 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     1681 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     1741 cctcactcct tctctaggcg ccggaattag atctctcgag ccagggtccc tgcccgctag
     1801 gcaagttgca ctcatggcac ctccaagtga agagacgccc ctgatccctc agcgttcatg
     1861 cagcctcttg tccacggagg ctggtgccct gcatgtgctg ctgcccgctc ggggccccgg
     1921 gcccccccag cgcctatctt tctcctttgg ggaccacttg gctgaggacc tgtgcgtgca
     1981 ggctgccaag gccagcggca tcctgcctgt gtaccactcc ctctttgctc tggccacgga
     2041 ggacctgtcc tgctggttcc ccccgagcca catcttctcc gtggaggatg ccagcaccca
     2101 agtcctgctg tacaggattc gcttttactt ccccaattgg tttgggctgg agaagtgcca
     2161 ccgcttcggg ctacgcaagg atttggccag tgctatcctt gacctgccag tcctggagca
     2221 cctctttgcc cagcaccgca gtgacctggt gagtgggcgc ctccccgtgg gcctcagtct
     2281 caaggagcag ggtgagtgtc tcagcctggc cgtgttggac ctggcccgga tggcgcgaga
     2341 gcaggcccag cggccgggag agctgctgaa gactgtcagc tacaaggcct gcctaccccc
     2401 aagcctgcgc gacctgatcc agggcctgag cttcgtgacg cggaggcgta ttcggaggac
     2461 ggtgcgcaga gccctgcgcc gcgtggccgc ctgccaggca gaccggcact cgctcatggc
     2521 caagtacatc atggacctgg agcggctgga tccagccggg gccgccgaga ccttccacgt
     2581 gggcctccct ggggcccttg gtggccacga cgggctgggg ctgctccgcg tggctggtga
     2641 cggcggcatc gcctggaccc agggagaaca ggaggtcctc cagcccttct gcgactttcc
     2701 agaaatcgta gacattagca tcaagcaggc cccgcgcgtt ggcccggccg gagagcaccg
     2761 cctggtcact gttaccagga cagacaacca gattttagag gccgagttcc cagggctgcc
     2821 cgaggctctg tcgttcgtgg cgctcgtgga cggctacttc cggctgacca cggactccca
     2881 gcacttcttc tgcaaggagg tggcaccgcc gaggctgctg gaggaagtgg ccgagcagtg
     2941 ccacggcccc atcactctgg actttgccat caacaagctc aagactgggg gctcacgtcc
     3001 tggctcctat gttctccgcc gcagccccca ggactttgac agcttcctcc tcactgtctg
     3061 tgtccagaac ccccttggtc ctgattataa gggctgcctc atccggcgca gccccacagg
     3121 aaccttcctt ctggttggcc tcagccgacc ccacagcagt cttcgagagc tcctggcaac
     3181 ctgctgggat ggggggctgc acgtagatgg ggtggcagtg accctcactt cctgctgtat
     3241 ccccagaccc aaagaaaagt ccaacctgat cgtggtccag agaggtcaca gcccacccac
     3301 atcatccttg gttcagcccc aatcccaata ccagctgagt cagataacat ttcacaagat
     3361 ccctgctgac agcctggagt ggcatgagaa cctgggccat gggtccttca ccaagattta
     3421 ccggggctgt cgccatgagg tggtggatgg ggaggcccga aagacagagg tgctgctgaa
     3481 ggtcatggat gccaagcaca agaactgcat ggagtcattc ctggaagcag cgagcttgat
     3541 gagccaagtg tcgtaccggc atctcgtgct gctccacggc gtgtgcatgg ctggagacag
     3601 caccatggtg caggaatttg tacacctggg ggccatagac atgtatctgc gaaaacgtgg
     3661 ccacctggtg ccagccagct ggaagctgca ggtggtcaaa cagctggcct acgccctcaa
     3721 ctatctggag gacaaaggcc tgccccatgg caatgtctct gcccggaagg tgctcctggc
     3781 tcgggagggg gctgatggga gcccgccctt catcaagctg agtgaccctg gggtcagccc
     3841 cgctgtgtta agcctggaga tgctcaccga caggatcccc tgggtggccc ccgagtgtct
     3901 ccgggaggcg cagacactta gcttggaagc tgacaagtgg ggcttcggcg ccacggtctg
     3961 ggaagtgttt agtggcgtca ccatgcccat cagtgccctg gatcctgcta agaaactcca
     4021 attttatgag gaccggcagc agctgccggc ccccaagtgg acagagctgg ccctgctgat
     4081 tcaacagtgc atggcctatg agccggtcca gaggccctcc ttccgagccg tcattcgtga
     4141 cctcaatagc ctcatctctt cagactatga gctcctctca gaccccacac ctggtgccct
     4201 ggcacctcgt gatgggctgt ggaatggtgc ccagctctat gcctgccaag accccacgat
     4261 cttcgaggag agacacctca agtacatctc acagctgggc aagggcaact ttggcagcgt
     4321 ggagctgtgc cgctatgacc cgctaggcga caatacaggt gccctggtgg ccgtgaaaca
     4381 gctgcagcac agcgggccag accagcagag ggactttcag cgggagattc agatcctcaa
     4441 agcactgcac agtgatttca ttgtcaagta tcgtggtgtc agctatggcc cgggccgcca
     4501 gagcctgcgg ctggtcatgg agtacctgcc cagcggctgc ttgcgcgact tcctgcagcg
     4561 gcaccgcgcg cgcctcgatg ccagccgcct ccttctctat tcctcgcaaa tctgcaaggg
     4621 catggagtac ctgggctccc gccgctgcgt gcaccgcgac ctggccgccc gaaacatcct
     4681 cgtggagagc gaggcacacg tcaagatcgc tgacttcggc ctagctaagc tgctgccgct
     4741 tgacaaagac tactacgtgg tccgcgagcc aggccagagc cccattttct ggtatgcccc
     4801 cgaatccctc tcggacaaca tcttctctcg ccagtcagac gtctggagct tcggggtcgt
     4861 cctgtacgag ctcttcacct actgcgacaa aagctgcagc ccctcggccg agttcctgcg
     4921 gatgatggga tgtgagcggg atgtccccgc cctctgccgc ctcttggaac tgctggagga
     4981 gggccagagg ctgccggcgc ctcctgcctg ccctgctgag gttcacgagc tcatgaagct
     5041 gtgctgggcc cctagcccac aggaccggcc atcattcagc gccctgggcc cccagctgga
     5101 catgctgtgg agcggaagcc gggggtgtga gactcatgcc ttcactgctc acccagaggg
     5161 caaacaccac tccctgtcct tttcatagga attccgcccc ccccccctaa cgttactggc
     5221 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5281 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5341 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5401 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5461 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5521 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5581 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5641 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5701 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     5761 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5821 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtctggcg agggcgaggg
     5881 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5941 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     6001 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6061 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     6121 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6181 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6241 caagcagaag aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcag
     6301 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6361 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     6421 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6481 gctgtacaag taatgaatta attaagaatt atcaagctta tcgataccgt cgacctgcag
     6541 ccaagcttat cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa
     6601 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
     6661 atacataact gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata
     6721 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     6781 tggtccccag atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg
     6841 gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc
     6901 tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac
     6961 tcggcgcgcc agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc
     7021 tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga
     7081 ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac
     7141 attctgaggg taggagtcga atattaagta atcctgactc aattagccac tgttttgaat
     7201 ccacatactc caatactcct gaaatagttc attatggaca gcgcagaaag agctggggag
     7261 aattgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7321 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7381 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8701 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9001 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9421 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9481 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9541 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9601 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9721 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9781 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9841 gac
//
