LOCUS       sequence-408541-        5763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prokaryotic expressing of AtVIP1 fused with a sequence encoding a 
            T4SS translocation tag in A. tumefaciens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Wang L, Zhao F, Liu H, Chen H, Zhang F, Li S, Sun T, Nekrasov V, 
            Huang S, Dong S
  TITLE     A modified Agrobacterium-mediated transformation for two oomycete 
            pathogens.
  JOURNAL   PLoS Pathog. 2023 Apr 21;19(4):e1011346. doi: 
            10.1371/journal.ppat.1011346. eCollection 2023 Apr.
  PUBMED    37083862
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2023-04-21"; volume: "19(4):e1011346. doi"; pages: 
            " 10.1371/journal.ppat.1011346. eCollection 2023 Apr"
COMMENT     Sequence Label: pV1F(Spec)
FEATURES             Location/Qualifiers
     source          1..5763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1369..2517)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2986..3777)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(3999..4103)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4171..4702
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4179..4789
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 atcaccaccc atacccatat tcttcttccc actatcttta ccactcacag aagctaaaat
       61 cgattcgata ttaaacgacg ccgaactcat ctctccatcc atagaattac tcctgctatg
      121 atgatgattc cctttcttct tctctcctga acttgtagct ataaacttct cctcagtaac
      181 acccaaatca tcgaagaaat cggaatcaac cgagaagcta cgaacatggc gaccgaatct
      241 agcttcgggt tttggaggaa gagaattagg aggaacaaca ccgttcgatg aggtttcttc
      301 cgaatcaacg gacatgggag aagcttgcgg ttgttgttgt gattgttgtg gtggtggtgg
      361 agcgttgagg aagtctagag aagagaaatc gatatcggaa ggatcgaata agaggagatc
      421 gtcgattgat tcgccggaga agaaggtttc agagcgagct cgacggtgat gagagatacg
      481 ttgacgtgga gcttcaggca tatgttctat ttcactgaga atcgtttgat ttggtcctct
      541 tcctcctcct tccatgtcga cactagtaga tctaagctta ccttatctcc ttagctcgca
      601 actaacaccg cctctcccgt tggaagaagt gcgttgtttt atgttgacga ttatcgggag
      661 ggtcggttac tcgaaaattt tcaattgctt ctttatgatt tcaattgaag cgaaaacctt
      721 cgctcggcgt cttggaacaa aacatggacc gagaaccgcg catccctgac aaagcaaccg
      781 gattgacaga ccgcagttgg tcaggtcggc ctcagaacga aaatgctcgc gatcaaactt
      841 tttggctgtc cgctggatgc tttttaacat tcgatgcgtc accgtggaat ctcccccggt
      901 cagcaggctc cacggtacgg tttgacacgc ggcggttcaa gcgaaattgg ttcttctagg
      961 ccatagataa tgatctgatc caaattcttg ctgctgccaa tttgactgcg gcgagaaagt
     1021 tcatcgggtt tctgtcatag cgcgtagcta cccctgttaa ctgtctgatt tcgcagaaaa
     1081 acgctcgacg agattacgct gtctgtaaac ccattcgctg aacgggaagc tttccttccg
     1141 attactcttg gcaggaatat tggcccatgc ctgcttgcgc tttgcaaacg ctcttatcgt
     1201 gttggtatca tatgccttgt ccgccagcag gaacgcactc ttagcgattg tttgtaaaaa
     1261 tgtttcgcca tgcggcggtc atgggcttga cacgctgtca gcgcaagacg gatcgccatg
     1321 gagtaaagcg ctggctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca
     1381 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc
     1441 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat
     1501 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa
     1561 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg
     1621 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt
     1681 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac
     1741 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt
     1801 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa
     1861 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag
     1921 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac
     1981 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa
     2041 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat
     2101 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc
     2161 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt
     2221 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc
     2281 gagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg
     2341 cacgctgtcg cgctcgatct tggccgtagc ttgctgggcc atcgagccga cggactggaa
     2401 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga
     2461 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca
     2521 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga
     2581 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta
     2641 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag
     2701 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg
     2761 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtagaaga
     2821 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2881 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2941 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttatt tgccgactac
     3001 cttggtgatc tcgcctttta cgtaatggac aaattcttcc aactgatctg cgcgcgaggc
     3061 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     3121 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     3181 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     3241 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     3301 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     3361 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     3421 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     3481 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     3541 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     3601 atcaagcctt acggtcaccg taaccagcaa atcaatatcg ctgtgtggct tcaggccgcc
     3661 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     3721 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
     3781 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     3841 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     3901 cccaaaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     3961 cgttcggtca aggttctgga ccagttgcgt gagcgcatac tcttcctttt tcaatattat
     4021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     4141 accattatta tcatgacatt aacctataaa agcgggccgg gagggttcga gaaggggggg
     4201 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
     4261 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
     4321 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
     4381 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
     4441 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
     4501 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
     4561 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
     4621 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
     4681 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
     4741 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
     4801 gaattctcat agaccgcgcg ttgatcgagg tctgtccgcc gacattaatt ccgctcgcgc
     4861 atcgtcgtga gccgttttaa tggatcgggc tatgggccga acttctgcca taacctcgag
     4921 agcggagatt tcattgaaca taatcggttc cgcaacgttg aagaccaagt tagggatatt
     4981 gttgatttta tggtccaagt ggttccctga caacctctct tctggttgac cgtcttgatg
     5041 ggtatacgct acgcccagga ctgggcgagt agcgacttca cttcgcattt gcgacagttg
     5101 cgcttcactt aattgttctc catctggaag tgttgctgcg tgccgcacgg catgagacaa
     5161 caatttcgcg tttcgcgaga ggattttgag gcgtgaagag aatttttcta aaggttctgt
     5221 tcgaaccgcg tcccttgtga aacgatggga tttgctaaaa ttagctatat tctcagccga
     5281 ctcgacaggg gtaccgcctc tcttggtgaa atccatgtag cttgggagcg atggctgccc
     5341 gtttgtactc atctgttggt tcgttttgtt cccaaactga ttcattgccg attgctgaga
     5401 tgagaattga gcacggttgt aagaatttcc attcccctga ggaatttctc cagctaccac
     5461 cttaagtcgg ttcagttcat cccgcagcgc ttcattcaaa gcatccctaa gttcagcttg
     5521 ttgctctaaa gcttgaagcc gcattttgag gtgtttattt tcagtgttca gctctgatgt
     5581 tcctctctgt aacatagtga cttgagcaga caatgtagta gcttcattct gaagtgtctg
     5641 aaccttcctc tctaactcac cagtatacct aatcttcctc tctttcgacc tcgccgcaga
     5701 ttgtctattc gctaaaatcc ttttagctct tttaggatca agcaaagcaa gctcagcaag
     5761 tct
//
