LOCUS       sequence-408542-        9298 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1803
FEATURES             Location/Qualifiers
     source          1..9298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(335..379)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    564..797
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1290..1415
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1462..1642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1643..1869)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1897..1917)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1897..1915)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1936..1952)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1936..1952)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1949..1971)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1960..1976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1984..1990,1991..2008,2009..2014))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2029..2050
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2167..2184)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2338..2926)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2418..2437)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3097..3888,3889..3957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3720..3739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3958..4062)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4088..4543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4437..4456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4670..4692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4684..4701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4685..4701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4711..4730
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4711..4729
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4750..4885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4925..5046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4936..4955)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4990..5009
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5124..5357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5429..6017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5535..5546
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5944..5964
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(7074..8252)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7083..8021
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7106..7126)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8390..8507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8550..9152)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8636..8656)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9133..9152
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(join(9184..9298,1..82))
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(join(9192..9298,1..104))
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      join(9198..9298,1..35)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
       61 gttgctgact aattgagacg catgctttgc atacttctgc ctgctgggga gcctggggac
      121 tttccacacc tggttgctga ctaattgaga tgcaatgcat gggtcccctc ggggttggga
      181 ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta
      241 cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat
      301 accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca
      361 ataattcttg ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta
      421 agcttgtgta attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct
      481 gttccagaga tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt
      541 ttccagagca accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca
      601 ggattcttgc ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg
      661 cctcaatagc cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg
      721 cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc
      781 caaggaacaa agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg
      841 ccttggtggg tgctactcct aatggttcaa tttttactac tttatattta tataattcac
      901 ttctccaatt gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt
      961 gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct
     1021 tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta
     1081 agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct
     1141 tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat
     1201 actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat
     1261 cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag
     1321 cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg
     1381 cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc
     1441 aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     1501 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     1561 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     1621 tctggtctaa ccagagagac ccgtttatgt atcgagctag gcacttaaat acaatatctc
     1681 tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag acccgtctgt tgccttccta
     1741 ataaggcacg atcgtaccac cttacttcca ccaatcggca tgcacggtgc tttttctctc
     1801 cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca agactacaag agtattgcat
     1861 aagactacat taagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg
     1921 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1981 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     2041 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     2101 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     2161 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2221 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2281 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2341 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2401 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2461 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2521 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2581 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2641 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2701 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2761 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2821 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2881 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2941 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     3001 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3061 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3181 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3241 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3541 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3661 gtcattctga gaatagtgta tacggcgacc gagttgctct tgcccggcgt caatacggga
     3721 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3961 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     4021 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4081 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     4141 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     4201 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     4261 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     4321 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     4381 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     4441 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     4501 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     4561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     4621 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     4681 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     4741 acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg
     4801 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
     4861 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac
     4921 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4981 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     5041 caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact gaataataag
     5101 atgacatgaa ctactatacg tactgctaga gattttccac actgactaaa agggtctgag
     5161 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     5221 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     5281 gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga
     5341 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
     5401 cttgtaagtc attggtctta aagtcgacgc ggggaggcgg cccaaaggga gatccgactc
     5461 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     5521 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     5581 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     5641 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     5701 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     5761 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     5821 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     5881 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     5941 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     6001 taatccagag gttgattgtc gacttaacgc gtaagcatgg tccagcacag ccgctctcgt
     6061 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggactagttc
     6121 attactagtt gctgtcattc ttgatgacga cctctaatcc gtggtgccgc aactgagctc
     6181 gaagcagcag attcttgttt ttaagatctt ccacctgttg tcgaagcacg tcattgtcca
     6241 gctgcagttg gtcaagtccc tgcagttctt cagacaagcg gtggttactc tgccgaagct
     6301 cctggatata atcacaagct ttggatagaa tcccaccttt actctggcca gacttggtgc
     6361 tctccataga gcagtctggg attatcttgg agagctgcac gatccagttg ttgatcttgt
     6421 ctcggcggcg acgctccact tcattatgct gagccctgcg tttctcatcc cgagtcgtcc
     6481 ggggagcttc tgacttcggg gaataagggt gagtcctagg ggcaattgag cgctggcttc
     6541 ctccctgcag tacttcttgt ggtgacatca tcacaaagaa ttgaccagtg ccaggagggg
     6601 tcgcctgccc cagcagtgcc tctgagccct gggtagtaac aacagcagct gtactccccg
     6661 atgtggtacc ccctgcccca tctcccactg ccgtgctggg gaagtaagta tagtgcgtct
     6721 cagcagctgt cccctccgtg tcaactgcat catcactggt gaaagcaccc tggatcaccg
     6781 cctgggtcat ggattgagtg gcagggtagc cactgatggc gccagttccc tcagtttggc
     6841 catccagctg cccctcagac acctggatca ccctgtacat cacctggccc ccattctcag
     6901 ttcggaagac gtacttgacg ttggggtcag ggaaggtggc agctgactgg atgctggcaa
     6961 tagccacact ggttgggtct tccccagtag ccactgcacc ttcctgaatc tgcactgtcc
     7021 cctcttccgt ttcagctgtt ttctgctgcc ccttcatggt ggcggtgcta gcctcacgac
     7081 acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat
     7141 ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact
     7201 tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct
     7261 aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac
     7321 tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt
     7381 gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg
     7441 ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc
     7501 cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga
     7561 gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg
     7621 cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc
     7681 agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat
     7741 caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac
     7801 cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc acgaggcgaa
     7861 ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg aagctcggga
     7921 tcaagaatca cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg gccataaccc
     7981 gtaaagaggc caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac
     8041 ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc
     8101 tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt
     8161 ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat
     8221 gtgcgctctg cccactgacg ggcaccggag ccaattccca ctcctttcaa gacctagaag
     8281 gtccattagc tgcaaagatt cctctctgtt taaaacttta tccatctttg cacctgcagg
     8341 cctcgagccg gcgccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa
     8401 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     8461 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     8521 ctgccatttg tctcaagatc tagaattcgt taactggcac cgggcttgcg ggtcatgcac
     8581 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     8641 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     8701 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     8761 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     8821 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     8881 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccacggcg
     8941 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     9001 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
     9061 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
     9121 gcgaggcgca ccgtgggctt gtactcggtc atggtaagct ccggtggtac ctctagaaag
     9181 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     9241 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcgga
//
