LOCUS       sequence-408570-        9664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0348
FEATURES             Location/Qualifiers
     source          1..9664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             complement(141..161)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     promoter        complement(610..1788)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          619..1557
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(642..662)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1926..2043
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2086..2688)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2172..2192)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2669..2688
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2720..2916)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2728..2938)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2734..2869
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3169..3213)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3398..3631
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4124..4249
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4296..4476
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4477..4703)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4731..4751)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4731..4749)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4770..4786)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4770..4786)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4783..4805)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4794..4810
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4818..4824,4825..4842,4843..4848))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4863..4884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5001..5018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5172..5760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5252..5271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5931..6722,6723..6791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6554..6573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6792..6896)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6922..7377)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7059..7080)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7271..7290
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7504..7526
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7518..7535
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7519..7535
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7545..7564
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7545..7563
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7584..7719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7759..7880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7770..7789)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7824..7843
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7958..8191
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8263..8851
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8369..8380
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8778..8798
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggctgtgacg ggaggtgcgg aggcggctgc ggagcagtag gcggttgctg atggaaatag
       61 tgtaccatgt gcggcggcgg cgggggcggc ggcggcggcg gctggggtgg gtggtggtga
      121 tggtggtggt gtgcggcagc gtggtgatga tggtggtggt gggggtggtg gtggtgatgg
      181 gggtgaaggt gccctcccgg accggcgccc ggcgcgctct tggtgcttcc cgcgtgcaag
      241 tgagtatggt cggcgcgcag caccttgaag cgcttgcgac gccgcaggaa gctgccgttc
      301 tcgaacatgt ccccgcagtc ggggtgcagc gcccagaagc tacccttgcc aggctggtcg
      361 ggcctccgcg gaatcttgat gaagcagtcg ttgaaggaga ggttgtggcg caggctgttc
      421 tgccagcgct gtgtgtgctc gcggtagtag gggaagcgct ccatgatgaa cttgtagatg
      481 tcgctcagcg gcagcatctt ctcggccgag tgctggattg ccatggcggt cagcgagatg
      541 taagagtagg gcggtttttg gtcgctgtac gagctcttcc ccggccgcgg catggtggcg
      601 gtgctagcct cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt
      661 gagaatgaac caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg
      721 ccaagctggc ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa
      781 cccctccccc caacctaaag acgacgtact ccaaaagctc aagaactaat cgaggtgcct
      841 ggacggcgcc cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggccct
      901 tttcctttgt gtgggtgact cacccgcccg ctctcccgag cgccgcgtcc tccattttga
      961 gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg
     1021 ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc
     1081 aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg
     1141 cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac
     1201 cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag
     1261 cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca
     1321 ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac
     1381 tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc
     1441 aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac
     1501 gcaagggcca taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg
     1561 tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata
     1621 cggttctccc ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt
     1681 taccccgcgc caccttctct aggcaccggt tcaattgccg acccctcccc ccaacttctc
     1741 ggggactgtg ggcgatgtgc gctctgccca ctgacgggca ccggagccaa ttcccactcc
     1801 tttcaagacc tagaaggtcc attagctgca aagattcctc tctgtttaaa actttatcca
     1861 tctttgcacc tgcaggcctc gagccggcgc caaagtggat ctctgctgtc cctgtaataa
     1921 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     1981 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     2041 aaattgtgga tgaatactgc catttgtctc aagatctaga attcgttaac tggcaccggg
     2101 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     2161 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     2221 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     2281 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     2341 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     2401 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     2461 ccagaccgcc acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     2521 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     2581 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     2641 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagctccgg
     2701 tggtacctct agaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     2761 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     2821 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     2881 ggggcgggac tatggttgct gactaattga gacgcatgct ttgcatactt ctgcctgctg
     2941 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcaat gcatgggtcc
     3001 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     3061 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     3121 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     3181 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     3241 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     3301 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     3361 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     3421 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     3481 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     3541 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     3601 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     3661 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     3721 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     3781 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     3841 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     3901 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     3961 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     4021 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     4081 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     4141 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     4201 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     4261 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     4321 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     4381 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     4441 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
     4501 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
     4561 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
     4621 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
     4681 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
     4741 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4801 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4861 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4921 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4981 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5581 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5641 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5701 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5761 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5821 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5881 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5941 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6001 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6061 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6121 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6181 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6241 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6301 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6361 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6421 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6481 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6541 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6601 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6661 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6721 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6781 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6841 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6901 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     6961 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7021 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7081 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7141 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7201 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7261 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7321 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     7381 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     7441 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     7501 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     7561 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7621 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     7681 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat
     7741 agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7801 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7861 taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa
     7921 tactgaataa taagatgaca tgaactacta tacgtactgc tagagatttt ccacactgac
     7981 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     8041 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     8101 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     8161 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     8221 agatctacag ctgccttgta agtcattggt cttaaagtcg acgcggggag gcggcccaaa
     8281 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
     8341 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
     8401 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca aggaaaggac gatgatttcc
     8461 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
     8521 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
     8581 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
     8641 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
     8701 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
     8761 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
     8821 tttcacaaat tttgtaatcc agaggttgat tgtcgactta acgcgtgagg tagcattccg
     8881 ggaccgcgcc tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag
     8941 ttacggacta gttcattact aggagtgcac tagcaccgag tgcaaggagc cgcccttgct
     9001 ttcggccgag gtaggggctg tgggctccag cagagaggca acgggcgagg ccgcggccgc
     9061 ggagcacacg gtggatggcg ccggaggctg ctgcgaagcc gcggggacag tgagccccgg
     9121 ctgcagcgcg gacacgggcg gcagcgcagg cgtgggcttg atgggcaccg gcatggcagg
     9181 caggctctgg cttgccgagt ggcacaggga cttgaccggg accccgaagg ccccatactc
     9241 cgggcctaca gggactccgg ctgcggctgc ggcggccgcg gcggcggcca ccgaatccat
     9301 gacgccaacg tgcggccaca cggagctgac gacgttgcca agctggccgg gcacggggta
     9361 gcccaggtgg tgcatgacgg acgccaaggg cagccctcca gcctgcagca cgcccttgta
     9421 gtcccggcca ataatgttct caatggcaaa ggggtgcttg aagcctgacg tggacgccgc
     9481 ggccgcggcg gcagcggcgg cggccgagcc cagcccgtag ggtgggaact gagacaggcg
     9541 tcccacgctg cccaccgcgg ctgccgcggc cgccgccgcc gccgccgcca cggccgccgc
     9601 ctcctgcatc ttgccggggt gagacggctg ctgaggctgc gactgctggg gcggttgctg
     9661 cggg
//
