LOCUS       sequence-408591-        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Wilkins KC, Schroeder T, Gu S, Revalde JL, Floor SN
  TITLE     A novel reporter for helicase activity in translation uncovers DDX3X
            interactions.
  JOURNAL   RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123.
  PUBMED    38697667
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079837"; journalName: "RNA"; date: "2024-07-16- 16"; 
            volume: "30"; issue: "8"; pages: "1041-1057"
COMMENT     Sequence Label: KW54_HART_ODC1
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1131..1510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1511..1714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1729..1909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1956..2081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2574..2807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2992..3036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3303..3420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3557..3691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3923..4630,4631..4633,4634..4636))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDSTYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3962..3983)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4311..4330
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4570..4591
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4633..4642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4660..4698)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5810..5828)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6078..6097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6521..7109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6574..6594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6992..7003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7196..7429
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7501..7622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7662..7797
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7817..7836)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7818..7836)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7846..7863)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7846..7862)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7855..7877)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8004..8459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8091..8110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8301..8322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8485..8589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8590..8658,8659..9450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8808..8827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt tgtatccata
     1081 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt
     1141 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1201 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1261 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1321 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1381 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1441 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1501 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     1561 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     1621 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     1681 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg
     1741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1921 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1981 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2041 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2101 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2161 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2221 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2281 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2341 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2401 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2461 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     2521 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2581 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2641 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2701 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2761 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2821 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2881 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2941 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3001 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3061 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3121 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3181 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3241 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     3301 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3361 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3421 atcgatcacg agactagcct cgagagatct gatcataatc agccatacca catttgtaga
     3481 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
     3601 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
     3661 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtctt
     3721 aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca gtagctctga
     3781 cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat tggctgtcca
     3841 gcttagctcg caggggaggt ggtctggatc caccggtccg gtttaattaa cgtagcacgc
     3901 gtttagtcat ctagctagca gtgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     3961 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     4021 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     4081 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     4141 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     4201 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     4261 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     4321 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     4381 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     4441 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     4501 tcagggtcag cttgccgtag gtggaatcgc cctcgccctc gccggacacg ctgaacttgt
     4561 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     4621 cgcccttgct caccatggtg gctagcggtt cactaaacga gctctgctta tataggcctc
     4681 ccaccgtaca cgcctaccct cgagaagctt gatatcgaat tccacggggt tggggttgcg
     4741 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     4801 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     4861 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     4921 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     4981 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     5041 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     5101 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcgacgtcg
     5161 gcccgccggc gccccaccag ctcctcgcgg gcccgggttg gccaccgccg ggcccccgcc
     5221 cctcccccgg cggtgtcccg gccggaaccg atcgtggctg gtttgagctg gtgcgtctcc
     5281 atggcgaccc gccggtgata taagtaggga gcggcgtgcc gtggggcttt gtcagtccct
     5341 cctgtagccg ccgccgccgc cgcccgccgc ccctctgcca gcagctccgg cgccacctcg
     5401 ggccggcgtc tccggcgggc gggagccagg cgctgacggg cgcggcgggg gcggccgagc
     5461 gctcctgcgg ctgcgactca ggctccggcg tctgcgcttc cccatggggc tggcctgcgg
     5521 cgcctgggcg ctctgagatt gtcactgctg ttccaagggc acacgcagag ggatttggaa
     5581 ttcctggaga gttgcctttg tgagaagctg gaaatatttc tttcaattcc atctcttagt
     5641 tttccatagg aacatcaaga aatcatggtt tcaaaaggtg aagaagataa catggccatc
     5701 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     5761 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     5821 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     5881 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     5941 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     6001 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     6061 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     6121 gagagaatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     6181 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     6241 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     6301 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     6361 gagctgtaca agaagcttag ccatggcttc ccgccggagg tggaggagca ggatgatggc
     6421 gcgctgccca tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc agcctgtgct
     6481 tctgctagga tcaatgtgta gagtcgagtc tagagtcgac aatcaacctc tggattacaa
     6541 aatttgtaaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6601 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6661 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6721 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6781 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6841 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6901 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6961 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7021 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7081 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
     7141 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7201 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7261 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7321 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7381 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7441 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7621 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     7681 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7741 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7801 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     7861 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     7921 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     7981 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     8041 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     8101 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     8161 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     8221 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     8281 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     8341 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     8401 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     8461 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8581 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8641 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8701 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8761 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8821 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8881 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8941 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9001 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9061 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9121 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9181 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9241 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9301 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9361 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9421 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9481 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9541 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9601 gaaaagatca aaggatcttc
//
