LOCUS       sequence-408610-       15379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0707
FEATURES             Location/Qualifiers
     source          1..15379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11236..11824
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11289..11309)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11707..11718)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11896..12129
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12207..12328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12244..12263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12298..12317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12368..12503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12523..12542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12524..12542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12552..12569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12552..12568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12561..12583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12710..13165
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12797..12816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13007..13028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13191..13295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13296..13364,13365..14156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13514..13533)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14327..14915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14816..14835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15069..15086
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15203..15224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15239..15244,15245..15262,15263..15269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15277..15293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15282..15304
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15301..15317
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15301..15317
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15336..15356
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15338..15356
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtca agttcaggtt atcctcccaa
     4141 ccaaggagca ttcagcacag aacaaagtcg ttatcctcct cactctgtcc agtatacatt
     4201 tcccaacacc cgccaccagc aggagttcgc agtccctgat tatcgttcct ctcatcttga
     4261 agtgagtcag gcatcacagc ttttgcagca acagcagcag caacagcttc gaaggcgacc
     4321 ttccttgctt tcagaatttc acccaggttc tgacaggcct caagaaagga gaactagtta
     4381 tgaaccgttt catccaggcc catccccagt ggatcatgat tcactggaat cgaagcgacc
     4441 acgtctggaa caggtttctg attctcattt tcagcgtgtc agtgctgcgg ttttgccttt
     4501 agtgcacccg ctgccagaag ggctgagggc ttctgcagat gctaagaagg atccagcatt
     4561 cggaggcaaa catgaagctc catcctctcc aatttcgggg caaccatgtg gagatgatca
     4621 aaatgcttca ccttcaaaac tctcaaagga agagttaata cagagtatgg atcgtgtaga
     4681 tcgagaaatt gcaaaagtag aacagcagat ccttaaactg aaaaagaaac aacaacagct
     4741 tgaagaagag gcagctaaac ctcctgagcc tgagaagccc gtgtcccctc ctcctgtgga
     4801 gcagaaacac cgcagtattg tccaaattat ttatgatgag aatcggaaaa aagcagaaga
     4861 agctcataaa atttttgaag gtcttggccc aaaagttgaa ctgccactgt ataaccagcc
     4921 atcagatacc aaggtgtacc atgagaacat caagacaaac caggtgatga ggaaaaaact
     4981 cattttattt tttaaaagaa gaaatcatgc aagaaaacaa agggaacaaa aaatctgcca
     5041 gcgttatgat cagctcatgg aggcatggga gaaaaaagtg gacagaatag aaaataatcc
     5101 tcggaggaaa gctaaagaaa gcaaaacaag ggaatactat gaaaagcagt ttccagaaat
     5161 tcgaaaacaa agagaacagc aagaaagatt tcagcgagtt gggcagaggg gagctggtct
     5221 ttcagccacc attgctagga gtgagcatga gatttctgaa attattgatg ggctctctga
     5281 gcaggagaat aatgagaaac aaatgcggca gctctctgtg attccaccta tgatgtttga
     5341 tgcagaacaa agacgagtca agttcattaa catgaatggg cttatggagg accctatgaa
     5401 agtgtataaa gataggcagt ttatgaatgt ttggactgac catgaaaagg agatctttaa
     5461 ggacaagttt atccagcatc caaaaaactt tggactaatt gcatcatact tggagaggaa
     5521 gagtgttcct gattgtgttt tgtattacta tttaaccaag aaaaatgaga attataaagc
     5581 cctcgtcaga aggaattatg ggaaacgcag aggcagaaac cagcaaattg ctcgaccctc
     5641 gcaagaagaa aaagtagaag aaaaagaaga ggataaagca gaaaaaacag aaaaaaaaga
     5701 agaagaaaag aaagatgaag aggaaaaaga tgaaaaagaa gactccaaag aaaataccaa
     5761 ggaaaaggac aagatagatg gtacagcaga agaaactgag gaaagagagc aagccacacc
     5821 ccgggggcga aagactgcca acagtcaggg ccgccgtaag ggccggatca ccaggtccat
     5881 gacaaacgaa gctgcagctg ccagtgctgc agccgcagcg gctactgaag agcccccacc
     5941 acctctgcca ccgccaccag aacccatttc tacagagcct gtggagacct ctcgatggac
     6001 agaagaagaa atggaagttg ctaaaaaagg tctagtagaa catggtcgta actgggcagc
     6061 aattgctaaa atggtgggaa cgaaaagtga agctcaatgt aaaaacttct attttaacta
     6121 taaaaggcga cacaatcttg acaacctctt acagcagcat aaacagaaaa cttcacgaaa
     6181 acctcgtgaa gagcgagatg tgtctcaatg tgaaagtgtc gcttccactg tttctgctca
     6241 ggaggatgaa gatattgaag cctccaatga agaagaaaat ccagaagaca gcgaaggtgc
     6301 agaaaatagt tctgatacag aaagtgctcc ttctccttca ccagttgaag ctgtcaagcc
     6361 cagcgaggac agtcctgaaa atgctacttc tcgaggaaac acagaacctg cggttgagct
     6421 tgagcccacc acggaaactg cacccagtac atctccctcc ttagcagttc caagtacaaa
     6481 accagctgaa gatgaaagtg tggagaccca ggtgaatgac agcatcagtg ctgagacagc
     6541 agagcagatg gatgtagatc agcaggagca cagtgctgaa gagggttctg tttgtgatcc
     6601 cccacccgct accaaagctg actctgtgga cgttgaagtg agggtgccag aaaaccatgc
     6661 atctaaagtt gaaggtgata ataccaaaga aagagacttg gatagagcca gtgagaaggt
     6721 ggaacctaga gatgaagatt tggtggtagc tcagcaaata aatgcccaaa ggcccgagcc
     6781 ccagtcagac aatgattcca gtgccacgtg cagcgctgat gaggatgtgg atggagagcc
     6841 agagaggcag agaatgtttc ctatggactc aaagccttca ctgttaaacc ccactggatc
     6901 tatactcgtc tcatctccgt taaaaccaaa tccactggat ctgccacagc ttcagcatcg
     6961 agctgctgtt atcccaccaa tggtatcctg caccccatgt aacataccaa ttggaacccc
     7021 agtgagcggc tatgctctct accagcgaca cattaaagca atgcatgagt cagcactcct
     7081 ggaggagcag cggcagagac aagaacagat agatttggaa tgtagaagtt ctacaagtcc
     7141 atgtggcaca tccaagagtc caaacagaga gtgggaagtc cttcagcctg ctccacatca
     7201 agtgataact aatctccctg aaggcgttcg gcttccgaca actcgaccaa ccaggccacc
     7261 gccccctctc atcccgtcat ccaaaaccac agtggcttca gaaaaaccat cttttataat
     7321 gggaggctcc atctcacagg gaacaccagg cacttatttg acttctcata atcaggcttc
     7381 ctacactcaa gaaacaccca agccgtcagt gggatctatc tctcttggac tgccacggca
     7441 acaggaatct gccaaatcag ctactttgcc ctacatcaag caggaagaat tttctccccg
     7501 aagccaaaac tcacaacctg agggtctgtt ggtcagggcc caacatgaag gtgtagtcag
     7561 aggtaccgca ggagccatac aagaaggaag tataactcgg ggaactccaa ccagcaaaat
     7621 ttcagtggag agcattccat ccctacgggg ctctatcact cagggcaccc cggctctgcc
     7681 ccagactggc ataccaacag aggctttggt gaaggggtcc atttcgagaa tgcccattga
     7741 agacagcagt cctgagaaag gcagagagga agctgcatcc aaaggccatg ttatttatga
     7801 aggcaaaagt ggacatatct tgtcatatga taatattaag aatgcccgag aagggactag
     7861 gagtccaaga acagctcatg aaatcagttt aaagagaagc tatgaatcag tggaaggaaa
     7921 tataaagcaa gggatgtcaa tgagggagtc tcctgtatca gcaccgttag aggggctgat
     7981 atgccgagca ttacccaggg ggagtcctca ttctgacctc aaagaaagga ctgtattgtc
     8041 tggctccata atgcagggga caccaagagc aacaactgaa agctttgaag atggccttaa
     8101 atatcccaaa caaattaaaa gggaaagtcc tcccatacga gcatttgaag gtgccattac
     8161 caaaggaaaa ccatatgatg gcatcaccac catcaaagaa atggggcgtt ccattcatga
     8221 gattccaagg caagatattt taactcagga aagtcggaaa actccagaag tggtccagag
     8281 cacacggccg ataattgagg gttccatttc ccagggcaca ccaataaagt ttgacaacaa
     8341 ctcaggtcaa tctgccatca aacacaatgt caaatcctta atcacggggc ctagcaaact
     8401 atcccgtgga atgcctccgc tggaaattgt gccagagaac ataaaagtgg tagaacgggg
     8461 aaaatatgag gatgtgaaag caggcgagac cgtgcgttcc cggcacacgt cagtggtaag
     8521 ctctggcccc tccgttctta ggtccacact gcatgaagct cccaaagcac aactgagccc
     8581 tgggatttat gatgacacca gtgcacggag gacccctgtg agttatcaaa acaccatgtc
     8641 cagaggctca cccatgatga acagaacttc tgatgttaca atttcttcta acaagtctac
     8701 caatcatgaa aggaaatcga cactgacccc tacccagagg gaaagtatcc cagcgaagtc
     8761 tccagtgcct ggggtggacc ctgtcgtgag ccacagtccg tttgatcccc atcacagagg
     8821 cagcactgca ggcgaggttt atcggagcca cctgcccacg cacttggatc cagccatgcc
     8881 ttttcacagg gctttggatc ctgcagcggc tgcttacctg tttcagagac agctttcacc
     8941 aactccaggt tacccaagtc agtatcagct ttacgcaatg gagaacacaa gacagacaat
     9001 cttaaatgat tacattacct cacaacagat gcaagtgaac ttgcgtccag atgtggccag
     9061 aggactctcc ccaagagagc agccactggg tctcccatac ccagcaacga gaggaatcat
     9121 tgacctgacc aatatgcctc caacaatttt agtgcctcat ccagggggaa caagcactcc
     9181 tcccatggac agaatcactt atattcctgg tacacagatt actttccctc ccaggccgta
     9241 caactctgct tccatgtctc caggacaccc aacacacctt gcagctgctg caagtgctga
     9301 gagggaacgg gaacgggagc gggagaagga gcgggagcgg gaacggattg ctgcagcttc
     9361 ctccgacctc tacctgcggc caggctcaga acagcctggc cgacctggca gtcatggata
     9421 tgttcgctcc ccttcccctt cagtaagaac tcaggagacc atgttgcaac agagacccag
     9481 tgttttccaa ggaaccaatg gaaccagtgt aatcacacct ttggatccaa ctgctcagct
     9541 acgaatcatg ccactgcctg ctgggggccc ttcaataagc caaggcctgc cagcctcccg
     9601 ttacaacact gctgcggatg ccctggctgc tcttgtggat gctgcagctt ctgcacccca
     9661 gatggatgtg tccaaaacaa aagagatatc ttctcacagg tatgaaacac ctagcgatgc
     9721 tattgaggtg ataagtcctg ccagctcacc tgcgccaccc caggagaaac tgcagaccta
     9781 tcagccagag gttgttaagg caaatcaagc ggaaaatgat cctaccagac aatatgaagg
     9841 accattacat cactatcgac cacagcagga atcaccatct ccccaacaac agctgccccc
     9901 ttcttcacag gcagagggaa tggggcaagt gcccaggacc catcggctga tcacacttgc
     9961 tgatcacatc tgtcaaatta tcacacaaga ttttgctaga aatcaagttt cctcgcagac
    10021 tccccagcag cctcctactt ctacattcca gaactcacct tctgctttgg tatctacacc
    10081 tgtgaggact aaaacatcaa accgttacag cccagaatcc caggctcagt ctgtccatca
    10141 tcaaagacca ggttcaaggg tctctccaga aaatcttgtg gacaaatcca ggggaagtag
    10201 gcctggaaaa tccccagaga ggagtcacgt ctcttcggag ccctacgagc ccatctcccc
    10261 accccaggtt ccggttgtgc atgagaaaca ggacagcttg ctgctcttgt ctcagagggg
    10321 cgcagagcct gcagagcaga ggaatgatgc ccgctcacca gggagtataa gctacttgcc
    10381 ttcattcttc accaagcttg aaaatacatc acccatggtt aaatcaaaga agcaggagat
    10441 ttttcgtaag ttgaactcct ctggtggagg tgactctgat atggcagctg ctcagccagg
    10501 aactgagatc tttaatctgc cagcagttac tacgtcaggc tcagttagct ctagaggcca
    10561 ttcttttgct gatcctgcca gtaatcttgg gctggaagac attatcagga aggctctcat
    10621 gggaagcttt gatgacaaag ttgaggatca tggagttgtc atgtcccagc ctatgggagt
    10681 agtgcctggt actgccaaca cctcagttgt gaccagtggt gagacacgaa gagaggaagg
    10741 ggacccatca cctcattcag gaggagtttg caaaccaaag ctgatcagca agtcaaacag
    10801 caggaaatct aagtctccta tacctgggca aggctactta ggaacggaac ggccctcttc
    10861 agtctcctct gtacattcag aaggggatta ccataggcag acgccagggt gggcctggga
    10921 agacaggccc tcttcaacag gctcaactca gtttccttat aaccctctga ctatgcggat
    10981 gctcagcagt actccaccaa caccgattgc atgtgctccc tctgcggtga accaagcagc
    11041 tcctcaccaa cagaacagga tctgggagcg agagcctgcc ccactgctct cagcacagta
    11101 cgagaccctg tcggatagtg atgactagta atgaactagt ccgtaacttg aaagtatttc
    11161 gatttcttgg ctttatatat cttgtggaaa ggacgactgg taatcgcgca taaccaaaca
    11221 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
    11281 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
    11341 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
    11401 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
    11461 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
    11521 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
    11581 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
    11641 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
    11701 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
    11761 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
    11821 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    11881 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    11941 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    12001 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    12061 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    12121 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
    12181 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    12241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12301 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    12361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    12421 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    12481 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    12541 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    12601 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    12661 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    12721 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    12781 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    12841 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    12901 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    12961 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    13021 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    13081 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    13141 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    13201 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    13261 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    13321 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    13381 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    13441 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    13501 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    13561 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    13621 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    13681 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    13741 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    13801 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    13861 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    13921 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    13981 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    14041 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    14101 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    14161 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    14221 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    14281 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    14341 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    14401 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    14461 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    14521 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    14581 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    14641 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    14701 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    14761 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    14821 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    14881 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    14941 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    15001 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    15061 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    15121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    15181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    15241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    15301 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    15361 aaaagctgga gctgcaagc
//
