LOCUS       sequence-408617-        9915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-q ONE-GO
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..58
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(962..1672,1673..1675,1676..1678))
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1004..1025)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1353..1372
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1612..1633
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(2101..2297)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2109..2319)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2115..2250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    2387..2502
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2765..2809)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2994..3227
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3724..3849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3896..4529
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4697..4831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4721..4740)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4775..4794
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4879..4983
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4984..5052,5053..5844)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5202..5221)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6015..6603
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6504..6523
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6757..6774
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6891..6912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6927..6932,6933..6950,6951..6957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6965..6981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6970..6992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6989..7005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6989..7005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             7133..7766
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7973..8561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8079..8090
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8488..8508
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8575..9090)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(9523..9543)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     misc_feature    join(9554..9562,9563..9565,9566..9915,1..225)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9727..9746)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg
       61 gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg
      121 ccaaaagacg gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc
      181 aagcggcttc ggccagtaac gttagggggg ggggagggag aggggcggcg gccgcttaga
      241 ccagattgta ctccttcagg ttcagctgca ggatggtgtc cttgacggct gcaaagacga
      301 agcggatgtt ctcggtatct gtggcgcacg tgaagtggga gtagatgatt ttgtcactgt
      361 cggggttcag gtccacgaac attttcagga tgaattctcg agctgcctgg gcatctctct
      421 ggggtccatc atattctggg aagtagtcga ctaggtggga atacatgatt ttctcctcta
      481 gaagatcttt cttgtttaag aacagaatca cagaggagtt ctggaaccag gggtaggtga
      541 taattgttct aaagagtgct ttgctctcct ccatgcggtt ctcattgtct gactccacaa
      601 gaacttgatc atattcgcta agcgctacta gaaacatgat ggaggtgaca ttttcaaagc
      661 agtgtatcca ttttcttctc tctgaccttt ggccccctac atcgaccatt ctgaaaatga
      721 cactttgtaa gtcaaagggg tattcgatga tccctgtagt ggggactcga actctaagca
      781 cgtcttgctc tgtcggcatg tactcagggt cggctacacg gtccaagtca ttcagatagt
      841 atttggtaga gtcagataac tgatattccc gtcgtctgtc gtagcactcc tggattccag
      901 gatcattcca caagctcttt attgcatcta catatggatt ctcggatcct ccaccgccag
      961 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     1021 ccatgtgatc gcgcttctcg ttggggtctt tgctcagttt ggactggtag ctcaggtagt
     1081 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     1141 cgagctgcac gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
     1201 tcttctgctt gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     1261 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     1321 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     1381 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     1441 tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg
     1501 tgggccaggg cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt
     1561 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     1621 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     1681 atccgccacc tcctgaaaaa gcagacacct tctccacatc aacctctcga accaattgtg
     1741 catgagcctt attgtgttca tacttgtatg ggatcttgag tgtgtccatc gctctgatca
     1801 tggcctgcat ggccgtgaag atgttctgat acaccagctt ggtgaagccg cgcttgtctt
     1861 cgtcagagta gcccgacccg tggatgatcc tcatctgctt gatgaaggtg ctcttgccac
     1921 tctcccctgt ccccagcagc agcagcttga gttcccggcg ggcgtcgcgc ttgtccctgc
     1981 gcagctgccg ctcgatctcg tcgttgatcc tccgggcttc cttggcctcc tcgctcaggc
     2041 agcacgccat gatggactcc agagtcatgg atccgcggcc gctctagaac tagtctcgag
     2101 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     2161 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     2221 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     2281 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     2341 acaccatcga taaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     2401 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     2461 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaggcgatac cgtcgagatc
     2521 cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt
     2581 cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc
     2641 cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc
     2701 ctcctactat cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat
     2761 tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt
     2821 tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc
     2881 actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat
     2941 tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct
     3001 gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc
     3061 agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct
     3121 gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc
     3181 ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc
     3241 ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa
     3301 tttttactac tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc
     3361 caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc
     3421 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     3481 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     3541 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt
     3601 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     3661 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     3721 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     3781 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     3841 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     3901 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     3961 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4021 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4081 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     4141 caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag ctcgatatca
     4201 gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct
     4261 gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg
     4321 gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg
     4381 tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta
     4441 gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     4501 tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat atttaacaaa
     4561 tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt aaatgaaacc
     4621 acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga ttttggttag
     4681 cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     4741 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4801 tgtaaccatt ataagctgca ataaacaagt tgatctcccg atccgtcgac gtcaggtggc
     4861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6781 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6841 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6901 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6961 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7021 ctctagctag aggtcgacgg taaacgctag caatgtcaag gcctctcact ctctgatatt
     7081 catttctttg caagttataa atactgaata ataagatgac atgaactact actgctagag
     7141 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     7201 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     7261 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag
     7321 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag
     7381 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca
     7441 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc
     7501 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg
     7561 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac
     7621 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt
     7681 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct
     7741 tcgttgggag tgaattagcc cttccagtcc cctcttttct tttaaaaagt ggctaagatc
     7801 tacagctgcc ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac
     7861 ctcctcctcc tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct
     7921 acttgtgatt gctccatgtt tttctaggtc tcgactgcag aattaattcc aggcggggag
     7981 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     8041 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     8101 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac
     8161 gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     8221 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     8281 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     8341 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     8401 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     8461 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     8521 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgttccagac gcgtttacgc
     8581 cagaatgcgt tcgcacagcc gccagccggt cactccgttg atggttactc ggaacagcag
     8641 ggagccgtcg gggttgatca ggcgctcgtc gataattttg ttgccgttcc acagggtccc
     8701 tgttacagtg atctttttgc cgtcgaacac ggcgatgcct tcatacggcc gtccgaaata
     8761 gtcgatcatg ttcggcgtaa ccccgtcgat taccagtgtg ccatagtgca ggatcacctt
     8821 aaagtgatga tcatccacag ggtacaccac cttaaaaatt ttttcgatct ggcccatttg
     8881 gtcgccgctc agaccttcat acgggatgat gacatggatg tcgatcttca gcccattttc
     8941 accgctcagg acaatccttt ggatcggagt tacggacacc ccgagattct gaaacaaact
     9001 ggacacacct ccctgttcaa ggacttggtc caggttgtag ccggctgtct gtcgccagtc
     9061 cccaacgaaa tcttcgagtg tgaagaccat ggatcccttc cactggcaaa accgtgtgaa
     9121 tttatcgctc tcgatgaatt tctggaacac gtctcctcgg aggttctggc aaatttcttc
     9181 aatatatggc tggaagagat ccggaggcac ctgcttcttc accagatggc cctggacgtg
     9241 ctcaatggcg ctcttcgaga aaggatgtga gcaggccagc agctccttca tgatgtacgt
     9301 gtcgaagatc tctcggctgc agaccaggcg ctcctcctct gtctccagct tctcgtattt
     9361 cttgatctcc tcgtagaact ctaccaaggg cttggcctcc tccaggtgct tcaggcagaa
     9421 gtctcggaaa agcaggtacc ccagcttctg ggagaagatc ttctcaaaag tcacctcgcc
     9481 ccggtcctcc aggtacttaa gcttgattcc ggaattcgac gtcttgctct tgctactccc
     9541 catctttggt acgggttgtg gcaagcttat catcgtgttt ttcaaaggaa aaccacgtcc
     9601 ccgtggttcg gggggcctag acgttttttt aacctcgact aaacacatgt aaagcatgtg
     9661 caccgaggcc ccagatcaga tcccatacaa tggggtacct tctgggcatc cttcagcccc
     9721 ttgttgaata cgcttgagga gagccatttg actctttcca caactatcca actcacaacg
     9781 tggcactggg gttgtgccgc ctttgcaggt gtatcttata cacgtggctt ttggccgcag
     9841 aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa gggtcgctac agacgttgtt
     9901 tgtcttcaag aagct
//
