LOCUS       sequence-408619-       10181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-SV40-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    116..231
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        299..509
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        321..517
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      368..503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1258..1277)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1605..1626
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2443..3017,3018..3020,3021..3029)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2610..2627)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2837..2856
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3040..3060
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3709..4224
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4238..4826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4291..4311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4709..4720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5033..5666
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5794..5810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5794..5810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5807..5829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5818..5834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5842..5848,5849..5866,5867..5872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5887..5908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6025..6042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6196..6784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6276..6295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6955..7746,7747..7815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7578..7597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7816..7920)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7968..8102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8005..8024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8059..8078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8270..8903
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8950..9075
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9572..9805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9990..10034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
       61 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa
      121 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      181 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      241 ttacagggac agcagagatc cagtttatcg atggtgtgga aagtccccag gctccccagc
      301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      421 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      481 gtgaggaggc ttttttggag gcctaggctt ttgcaaactc gagactagtt ctagagcggc
      541 cgcggatcca tggcggactt cctgccgtcg cggtccgtgc tgtccgtgtg cttccccggc
      601 tgcctgctga cgagtggcga ggccgagcag caacgcaagt ccaaggagat cgacaaatgc
      661 ctgtctcggg aaaagaccta tgtgaagcgg ctggtgaaga tcctgctgct gggcgcgggc
      721 gagagcggca agtccacctt cctgaagcag atgcggatca tccacgggca ggacttcgac
      781 cagcgcgcgc gcgaggagtt ccgccccacc atctacagca acgtgatcaa aggtatgagg
      841 gtgctggttg atgctcgaga gaagcttcat attccctggg gagacaactc aaaccaacaa
      901 catggagata agatgatgtc gtttgatacc cgggccccgc ggggatcagg catggtttca
      961 aagggagaag agttgttcac aggggttgta ccgattcttg tagaacttga cggagatgtc
     1021 aacgggcaca agtttagtgt aagtggagaa ggcgagggcg atgcgaccta tggaaaactg
     1081 acccttaaat ttatttgtac tacgggtaaa ttgcccgttc cctggccgac attggtaact
     1141 acattcggat atggtttgat gtgctttgca aggtatccgg atcacatgaa gcagcatgat
     1201 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     1261 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1321 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1381 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1441 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1501 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1561 taccagtccg ccatgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1621 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg atcaggcccg
     1681 cggatggcag cccaaggaat ggtggaaaca agggttttct tacaatatct tcctgctata
     1741 agagcattat gggcagacag cggcatacag aatgcctatg accggcgtcg agaatttcaa
     1801 ctgggtgaat ctgtaaaata tttcctggat aacttggata aacttggaga accagagtat
     1861 atgccaacag aacaagatat tctgcttgcc agaagaccca ccaaaggcat ccatgaatac
     1921 gactttgaaa taaaaaatgt tcctttcaaa atggttgatg taggtggtca gagatcagaa
     1981 aggaaacgtt ggtttgaatg tttcgacagt gtgacatcaa tacttttcct tgtttcctca
     2041 agtgaatttg accaggtgct tatggaagat cgactgacca atcgccttac agagtctctg
     2101 aacatttttg aaacaatcgt caataaccgg gttttcagca atgtctccat aattctgttc
     2161 ttaaacaaga cagacttgct tgaggagaag gtgcaaattg tgagcatcaa agactatttc
     2221 ctagaatttg aaggggatcc ccactgctta agagacgtcc aaaaattcct ggtggaatgt
     2281 ttccggaaca aacgccggga ccagcaacag aagcccttat accaccactt caccactgct
     2341 atcaacacgg agaacatccg ccttgttttc cgtgacgtga aggatactat tctgcatgac
     2401 aacctcaagc agcttatgct acagtgatct aggcggccgc cgcccctctc cctccccccc
     2461 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     2521 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     2581 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     2641 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     2701 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     2761 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     2821 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     2881 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     2941 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     3001 aaaaacacga tgataagctt gccacaaccc gtaccaaaga tggggagtag caagagcaag
     3061 acgtcgaatt ccggaatcaa gcttctgatt attggcccag aggaagatta tgacccaggt
     3121 tatttcaaca atgagagtga catcatcttc caagatcttg aaaaactgaa gtcacatcca
     3181 gcttacttgg tagtttttct acgttacatc ctctctcagg cagaccctgg ccccctgctt
     3241 ttttatttgt gttcagaagt ttatcaacag acaaatccca aagattcccg aagtctgggg
     3301 aaagacatct ggaacatttt cctggagaaa aatgcgcctc tcagagtgaa gatccctgag
     3361 atgttgcagg ctgaaattga cctacgcctg cggaacaatg aggaccctcg caatgtgctc
     3421 tgtgaagctc aggaggcagt catgctggaa atccaggagc agatcaacga ctacagatcc
     3481 aagcgtactc tgggcctggg cagcctctat ggtgaaaatg acctgctagg cctggatggg
     3541 gaccctcttc gagaacgcca aatggctgag aagcagctgg ctgcccttgg agatatcttg
     3601 tccaaatatg aggaagatcg gagtgccccc atggactttg ctgttaatac ctttatgagc
     3661 cacgctggga tccgtcttcg ggagtctcga ggcggatcag gcggatccat ggtcttcaca
     3721 ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt
     3781 gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa
     3841 aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat
     3901 gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct
     3961 gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt
     4021 acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc
     4081 aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg
     4141 atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg
     4201 ctgtgcgaac gcattctggc gtaaacgcgt ctggaacaat caacctctgg attacaaaat
     4261 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4321 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4381 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4441 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4501 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4561 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4621 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     4681 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4741 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4801 gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa
     4861 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     4921 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     4981 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
     5041 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     5101 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     5161 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     5221 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     5281 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     5341 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     5401 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     5461 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     5521 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5581 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5641 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5701 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc
     5761 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg
     7981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     8101 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     8161 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     8221 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     8281 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     8341 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     8401 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     8461 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     8521 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     8581 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     8641 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     8701 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8881 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     8941 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9001 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9061 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9121 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9181 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9241 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9301 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9361 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     9421 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9481 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9541 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9601 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9661 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9721 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9781 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9841 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     9901 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9961 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
    10021 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
    10081 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
    10141 tgaatagagt taggcaggga tattcaccat tatcgtttca g
//
