LOCUS       sequence-408622-       11862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 11862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO
FEATURES             Location/Qualifiers
     source          1..11862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..59
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(1491..1515)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1512..1715)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1541..1561)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1716..2019
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2077..2192
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(2455..2499)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2684..2917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3414..3539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3586..4219
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4387..4521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4411..4430)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4465..4484
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4569..4673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4674..4742,4743..5534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4892..4911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5705..6293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6194..6213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6447..6464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6581..6602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6617..6622,6623..6640,6641..6647)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6655..6671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6660..6682
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6679..6695
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6679..6695
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             6823..7456
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7663..8251
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7769..7780
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8178..8198
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8496..9230)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     8689..8708
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8948..8969
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9072..9093)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(9237..9290)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(10311..10364)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(10374..10886)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(11493..11513)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     misc_feature    join(11513..11862,1..203)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(11515..11862,1..226)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11675..11694)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 ttccagagga actgcttcct tcacgacatt caacagacct tgcattcctt tggcgagagg
       61 ggaaagaccc ctaggaatgc tcgtcaagaa gacagggcca ggtttccggg ccctcacatt
      121 gccaaaagac ggcaatatgg tggaaaataa catatagaca aacgcacacc ggccttattc
      181 caagcggctt cggccagtaa cgttaggggg gggggaggga gaggggcggc ggccgcttat
      241 agcagctcgt actgacgaag gtgcatgcgc tgaatgatgt cacggcagtc gttgaacaca
      301 cggcggatgt tctcagtgtc cacagcgcag gtgaaatgag ggtagcagta gtgacgccca
      361 tctccactgg cagtgctgat cctcagaaac tcatctcgaa tgaagtactt ggcccgggtc
      421 acgcgtgggt cctctccggg ctcgggagta gcatcctcag gagtagtgta gcgagcaaat
      481 tctggaaagt agtcctcaat cttcgatttc ccagcaagga ctttctcagc gagcagatct
      541 tgcttgttga ggaacaggat cacagagatg gtgcgcagcc atctgttgtt ccagatgctc
      601 ttgaagaggt tcagagcctc ctgcaggcgg ttggtctggt tgtcctcccg gatgaccatg
      661 ttgtagctgc tgctggccac cacgaagatg atggcagtca catcgttgaa gcactggatc
      721 cacttgcggc gttcatcgcg ctggccaccc acgtcaaaca tgtggaagtt gactttgtcc
      781 acctggaact tggtctcaaa gattccagaa gtcaggacac ggcagcgaag caggtcctga
      841 tcgctcggca catagtcagc ctgcttgatc acgtcgatct tgtccaggaa gtactgggca
      901 cagtcaatca gctggtactc gttggagcgt tcgtagcagg cacgcactcc ttcatcctcc
      961 cacagagcct tggcatgctc atagaattcg ggagggaagt caaagtcagg cacgttcatc
     1021 acactcagaa tgtagtccac tctgaactgg ttctcggggt tggccagctc cacggggggc
     1081 accaggttgc tcatggcggc cacaatggtt tcaatcgcct ctttcaggtt gtttttgatg
     1141 tcctgcactt tggttgcctt ctcactgtct ccattaaacc cattaacatg caggatcctc
     1201 atctgcttca caatggtgct tttaccagat tctccagcac ccagcagcag caggcggtgc
     1261 gtggcccggt agacctgctt gtccttctgc agctgcttct cgatcttttt gttggcctca
     1321 cgctgcgcct tctcctcgtt gcgctggtcc tcggtcttac tgttcccgag gcagcccatg
     1381 gatccgcggc cgctctagaa ctagtctcga ggaattcacc ggaaatagat cctctagtag
     1441 agtcggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg
     1501 ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
     1561 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
     1621 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
     1681 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
     1741 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     1801 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     1861 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     1921 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     1981 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccca agcttatcga
     2041 taaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
     2101 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
     2161 ctgtaccccc caatcccccc ttttctttta aaggcgatac cgtcgagatc cgttcactaa
     2221 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
     2281 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     2341 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     2401 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     2461 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     2521 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     2581 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     2641 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     2701 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     2761 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     2821 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     2881 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     2941 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     3001 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     3061 gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc
     3121 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     3181 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     3241 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat
     3301 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     3361 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     3421 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     3481 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     3541 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     3601 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     3661 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     3721 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa
     3781 gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc
     3841 ctccctggaa agtccccagc ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg
     3901 aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc
     3961 cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag
     4021 ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg tactagcttg
     4081 tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta gttctgctaa
     4141 tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttctttgg
     4201 gagtgaatta gcccttccaa ctactaagtt tgtagtacat atttaacaaa tacaatttct
     4261 ttaaaatgaa aataattcag aggaatcaca ggtttagagt aaatgaaacc acaggtaatt
     4321 ggcagtggta atagggtatg gggtgggaag tttgggatga ttttggttag cttgagttat
     4381 ccagttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     4441 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     4501 ataagctgca ataaacaagt tgatctcccg atccgtcgac gtcaggtggc acttttcggg
     4561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6361 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6421 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6481 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6541 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6601 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6661 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
     6721 aggtcgacgg taaacgctag caatgtcaag gcctctcact ctctgatatt catttctttg
     6781 caagttataa atactgaata ataagatgac atgaactact actgctagag attttccaca
     6841 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     6901 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     6961 cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag gcaaaaagca
     7021 gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag gccacgcctc
     7081 cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca gttcttgaag
     7141 tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc aaacctccac
     7201 tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagctg
     7261 gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac tagcttgtag
     7321 caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt ctgctaatca
     7381 gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag
     7441 tgaattagcc cttccagtcc cctcttttct tttaaaaagt ggctaagatc tacagctgcc
     7501 ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc
     7561 tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt
     7621 gctccatgtt tttctaggtc tcgactgcag aattaattcc aggcggggag gcggcccaaa
     7681 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
     7741 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
     7801 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc
     7861 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
     7921 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
     7981 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
     8041 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
     8101 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
     8161 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
     8221 tttcacaaat tttgtaatcc agaggttgat tgttccagac gcgtttaaag gatggcacag
     8281 aaaaacttct tctccctaaa cgggttttct gaagccggaa caggggtcag gagggggtct
     8341 tccttggcat gtgcttcaca gtaggccatc aaatctgcag ctgccttgga cacctttatc
     8401 ctgtcgatat tggcttccat cttaagctgc tctaccagct tcctggcttg tgctatgctg
     8461 gcggtgttgt tgctggccat agatctgagt ccggactcga tgttgtggcg gatcttgaag
     8521 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg gctgttgtag
     8581 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     8641 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     8701 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     8761 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcag gccgtagccc
     8821 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatcagcttc
     8881 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     8941 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     9001 cccttgctca ccatgccacc gctgccaccc ttgtacagct cgtccatgcc gagagtgatc
     9061 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
     9121 ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
     9181 atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtccat accggtaggg
     9241 ccgggattct cctccacgtc accgcatgtt agaagacttc ctctgccctc gttccagatc
     9301 ttgaggaagc tatcccagga ccctgtcgcc acagccatgc catcgtcagt cacgcccagg
     9361 cagctgacgc ggttgtcatg cccagccaag acacctgccc ggtcggcttt gagtgcatcc
     9421 cagacgttgc agttgaagtc gtcgtaccca gcaaggagga ggcgcccgct cttggagaag
     9481 gagacagagg tgatcccgca gatgatgttg tcatgggagt aagtcatgag ctcctggtca
     9541 gcacgaaggt caaacagcct gcaggtggcg tcgtctgagc cagtggcaaa tgcattgcca
     9601 tttggaaaga agcaaatggc attgatgtca gactcgtggc cagtgaaggt ctgccggcac
     9661 atgccttctc gcacatccca gagtttggct gaagcatcac aagcaccaga gacgaacagt
     9721 ctggtgtcag gagcaagaga aaggctcatg acatctccag tgtgtccggt aaacgtggtc
     9781 gtctgctggc cggtctcgat gtcccacagg gcacacgtgg tgtctccaga gctggtgacg
     9841 atctgattgt catccaggaa tcggcagcag gacaggtaac ctgtgtgtcc tgccagctca
     9901 cgactcacgc gcacgttccc ctcacgagtt ttcagattgt aaatggagca aatgttatcc
     9961 aggccaccgc aggccacata gttcccagaa ggggcatatg cacaggtcat gacccaggag
    10021 gagcgcagag ggatggcgtg gaccttgttg gtggtgtagc tgtcccagat gataagttta
    10081 ccatcctgcg aggcactgac gagaagcctg gagtctgtgc cccagtgcat ggcgtagatc
    10141 ttggccaggt gcccccgcag tgtcctcctc gtgcgcattt ggattcttcc cactgggtcg
    10201 atgttgtttg tgatctgaga gagagttgca tctgcacatg ctttcctggc gtctcgaatc
    10261 tggttcttaa gttgctcggc ctcctgccgt aactggtcaa gctcccccat tgggccggga
    10321 ttttcctcca cgtccccgca tgttagtaga cttcctctac cttctcctga tcccgccaga
    10381 atgcgttcgc acagccgcca gccggtcact ccgttgatgg ttactcggaa cagcagggag
    10441 ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc cgttccacag ggtccctgtt
    10501 acagtgatct ttttgccgtc gaacacggcg atgccttcat acggccgtcc gaaatagtcg
    10561 atcatgttcg gcgtaacccc gtcgattacc agtgtgccat agtgcaggat caccttaaag
    10621 tgatgatcat ccacagggta caccacctta aaaatttttt cgatctggcc catttggtcg
    10681 ccgctcagac cttcatacgg gatgatgaca tggatgtcga tcttcagccc attttcaccg
    10741 ctcaggacaa tcctttggat cggagttacg gacaccccga gattctgaaa caaactggac
    10801 acacctccct gttcaaggac ttggtccagg ttgtagccgg ctgtctgtcg ccagtcccca
    10861 acgaaatctt cgagtgtgaa gaccatggat ccacctccga ggccgttgct gttcctatgg
    10921 cagaggggag gctttgagag ttccacgacg gctgagcggg atttgttgag gaacttgggg
    10981 gctcgccgta gcagccgctg ggcctccatg aatgtctccg tcagctcttt cttccactgc
    11041 acaaactctg ggtcactctc acactgcaaa acgaactgct tccctccttt tattctcaac
    11101 aaaatgcact ttttgtcttt aatctgagtt tcttccacgg acacaatctg ttccattgtc
    11161 agtaaacttt gtcgcgactc gccttctcct ctccactcaa gtctgttcgg aaagaggtaa
    11221 aaatagcggc gctgccactg ggtcaggaaa gggttcccca gcttcagcat gtacccgtgc
    11281 acgatgcagt ctcttcccag ggcgtaatct tcttcgtggc caagctgctt atttttagct
    11341 ctcttcctgg cctcgatttt atccgtgtct gcatttactg cttcataaac tgtttccgcc
    11401 acttcctgct gccagcgctc agagatcacc agagggaagt tcttgtagag ttcttggtcg
    11461 caatcaagaa gcttgattcc ggaattcgac gtcttgctct tgctactccc catattatca
    11521 tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa
    11581 cctcgactaa acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg
    11641 gggtaccttc tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac
    11701 tctttccaca actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt
    11761 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
    11821 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gc
//
