LOCUS       sequence-408624-       10384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    189..304
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        346..793
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(1534..1553)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1881..1902
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2719..3293,3294..3296,3297..3305)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2886..2903)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3113..3132
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3316..3336
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3985..4500
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4514..5102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4567..4587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4985..4996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5309..5942
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6070..6086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6070..6086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6083..6105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6094..6110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6118..6124,6125..6142,6143..6148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6163..6184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6301..6318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6472..7060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6552..6571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7231..8022,8023..8091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7854..7873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8092..8196)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8244..8378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8281..8300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8335..8354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8546..9179
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9226..9351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9848..10081
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10266..10310
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
       61 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
      121 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      181 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      241 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      301 attttcgggt ttattacagg gacagcagag atccagttta tcgatagtgc aagtgggttt
      361 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
      421 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
      481 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
      541 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
      601 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
      661 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
      721 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
      781 cctgagagcg cagctcgaga ctagttctag agcggccgcg gatccatggc ggacttcctg
      841 ccgtcgcggt ccgtgctgtc cgtgtgcttc cccggctgcc tgctgacgag tggcgaggcc
      901 gagcagcaac gcaagtccaa ggagatcgac aaatgcctgt ctcgggaaaa gacctatgtg
      961 aagcggctgg tgaagatcct gctgctgggc gcgggcgaga gcggcaagtc caccttcctg
     1021 aagcagatgc ggatcatcca cgggcaggac ttcgaccagc gcgcgcgcga ggagttccgc
     1081 cccaccatct acagcaacgt gatcaaaggt atgagggtgc tggttgatgc tcgagagaag
     1141 cttcatattc cctggggaga caactcaaac caacaacatg gagataagat gatgtcgttt
     1201 gatacccggg ccccgcgggg atcaggcatg gtttcaaagg gagaagagtt gttcacaggg
     1261 gttgtaccga ttcttgtaga acttgacgga gatgtcaacg ggcacaagtt tagtgtaagt
     1321 ggagaaggcg agggcgatgc gacctatgga aaactgaccc ttaaatttat ttgtactacg
     1381 ggtaaattgc ccgttccctg gccgacattg gtaactacat tcggatatgg tttgatgtgc
     1441 tttgcaaggt atccggatca catgaagcag catgatttct tcaagtccgc catgcccgaa
     1501 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1681 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1741 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1801 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccat gagcaaagac
     1861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1921 ctcggcatgg acgagctgta caagggatca ggcccgcgga tggcagccca aggaatggtg
     1981 gaaacaaggg ttttcttaca atatcttcct gctataagag cattatgggc agacagcggc
     2041 atacagaatg cctatgaccg gcgtcgagaa tttcaactgg gtgaatctgt aaaatatttc
     2101 ctggataact tggataaact tggagaacca gagtatatgc caacagaaca agatattctg
     2161 cttgccagaa gacccaccaa aggcatccat gaatacgact ttgaaataaa aaatgttcct
     2221 ttcaaaatgg ttgatgtagg tggtcagaga tcagaaagga aacgttggtt tgaatgtttc
     2281 gacagtgtga catcaatact tttccttgtt tcctcaagtg aatttgacca ggtgcttatg
     2341 gaagatcgac tgaccaatcg ccttacagag tctctgaaca tttttgaaac aatcgtcaat
     2401 aaccgggttt tcagcaatgt ctccataatt ctgttcttaa acaagacaga cttgcttgag
     2461 gagaaggtgc aaattgtgag catcaaagac tatttcctag aatttgaagg ggatccccac
     2521 tgcttaagag acgtccaaaa attcctggtg gaatgtttcc ggaacaaacg ccgggaccag
     2581 caacagaagc ccttatacca ccacttcacc actgctatca acacggagaa catccgcctt
     2641 gttttccgtg acgtgaagga tactattctg catgacaacc tcaagcagct tatgctacag
     2701 tgatctaggc ggccgccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     2761 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     2821 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     2881 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     2941 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     3001 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     3061 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     3121 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     3181 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     3241 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca
     3301 caacccgtac caaagatggg gagtagcaag agcaagacgt cgaattccgg aatcaagctt
     3361 ctgattattg gcccagagga agattatgac ccaggttatt tcaacaatga gagtgacatc
     3421 atcttccaag atcttgaaaa actgaagtca catccagctt acttggtagt ttttctacgt
     3481 tacatcctct ctcaggcaga ccctggcccc ctgctttttt atttgtgttc agaagtttat
     3541 caacagacaa atcccaaaga ttcccgaagt ctggggaaag acatctggaa cattttcctg
     3601 gagaaaaatg cgcctctcag agtgaagatc cctgagatgt tgcaggctga aattgaccta
     3661 cgcctgcgga acaatgagga ccctcgcaat gtgctctgtg aagctcagga ggcagtcatg
     3721 ctggaaatcc aggagcagat caacgactac agatccaagc gtactctggg cctgggcagc
     3781 ctctatggtg aaaatgacct gctaggcctg gatggggacc ctcttcgaga acgccaaatg
     3841 gctgagaagc agctggctgc ccttggagat atcttgtcca aatatgagga agatcggagt
     3901 gcccccatgg actttgctgt taataccttt atgagccacg ctgggatccg tcttcgggag
     3961 tctcgaggcg gatcaggcgg atccatggtc ttcacactcg aagatttcgt tggggactgg
     4021 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     4081 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     4141 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     4201 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     4261 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     4321 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     4381 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     4441 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa
     4501 acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4561 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4621 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4681 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4741 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4801 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4861 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4921 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4981 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5041 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5101 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     5161 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5221 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5281 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     5341 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     5401 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     5461 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     5521 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     5581 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     5641 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     5701 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     5761 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5821 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5881 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5941 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6001 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     6061 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6121 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6181 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6241 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6301 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6361 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6421 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6481 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6541 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6601 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6661 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6721 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6781 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6841 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6901 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6961 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7021 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7081 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7141 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7201 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7261 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7321 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7381 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7441 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7501 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7561 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7621 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7681 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7741 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7801 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7861 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7921 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7981 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8041 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8101 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8161 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8221 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
     8281 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8341 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8401 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8461 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8521 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     8581 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8641 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8701 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8761 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8821 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8881 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8941 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     9001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9181 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     9241 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     9301 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9361 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9421 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9481 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9541 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9601 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9661 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     9721 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9781 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9841 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9901 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9961 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
    10021 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
    10081 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
    10141 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
    10201 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
    10261 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
    10321 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10381 tagg
//
