LOCUS       sequence-408630-        4577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen
            J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat
            KM, Brunet A
  TITLE     Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network
            of Proteins Involved in Mitosis.
  JOURNAL   Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1.
  PUBMED    22137581
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-12-23"; volume: "44(6)"; pages: "878-92. Epub 2011 Dec 
            1"
COMMENT     Sequence Label: pECE-HA-AMPKalpha2 wt
FEATURES             Location/Qualifiers
     source          1..4577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     152..172
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        227..331
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(332..400,401..1192)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(550..569)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1363..1951
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1852..1871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2105..2122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2387..2406
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     polyA_signal    2465..2599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2489..2508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2543..2562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4388..4414)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
       61 ctgctgggga gcctggggac tttccttgct gactaattga gatgcatgct ttgcatactt
      121 ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg
      181 ttctttccgc ctcagaaggt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      421 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      481 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      541 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
      601 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      661 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      721 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      781 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
      841 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
      901 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
      961 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1021 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1081 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1141 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1201 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2041 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2101 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     2161 gcatctgtgc ggtatttcac accgcatatc gacgctctcc cttatgcgac tcctgcatta
     2221 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     2281 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     2341 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     2401 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     2461 agaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2521 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2581 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2641 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac agatgtgata tggctgatta
     2701 tgatcattac ttatctagat caacgggcta aagtagtaat cagactggca cacatttcaa
     2761 aaaaatccat ggtgtgactg cccaggcgag gtgaaactga agacaatgtg cttccggtca
     2821 aagagccagt gagagagcca gaaagtgaat ggctctctgc agttgtggaa tcaaaacttg
     2881 atcttggtct gtgtaagcca gcagcagaac aggaacgctg aggtgttgag gaaccagatc
     2941 tctgctccac tacttcatca tcaatgcttt taaagtccaa aagatagctc ctgttatcaa
     3001 ccaggtaaag ttgtaagctc attttcacgt aattgccagt cactggattt tttcttctta
     3061 cacgaagatg gtatgcattc actaccttcc attcaaaatc cagctgcttc atagctcggt
     3121 aaacttcagc cataatgtca tacggtttgc tctgacttcg gattccaaga tgccacttgg
     3181 cttttttcac agctaaagat ttgggcttag tcgtattcag tgcatccaat ggacatcttg
     3241 ctttggggct gtctgctata agaggtggca tcctttctgg atgaggtttc aggcctgggg
     3301 gaatatgcat ggcactatca tccataaaag aaccagatgg aggactagag gcgaggtaga
     3361 actcactggc ttggttcatt attctccgat tgtcaatgat aagatgataa gccactgcaa
     3421 gctggtcttg agggtcacca ctatataaac tgttcattac ttctgattct gtacattcaa
     3481 atttttcaca cacttctttc acagcctcat catcaatgac gttagcatca taggaagggt
     3541 cttcaggaaa taagtaactg ggcaaatctt gtttaaacca ttcatgctct cttatgtctt
     3601 tgatagttgc tcgtttcagt gggtcaacct gcagcatatg catcaggaga gtggcgacag
     3661 aacgattgag atattctggg atataaaaga caccccctcg gatcttctta aataacgtag
     3721 gtacatgctc atcatcaaat gggagggtgc cacaaagaag agcatacaag ataacaccac
     3781 agctccagat atcaacttca ggacctgcat acaatctgcc tgagatgact tcaggtgctg
     3841 cataatttgg agatccgcaa ctagttctca gaaattcacc atctgacatc atattagata
     3901 atccgaaatc ggctatcttg gcattcatgt gtgcatccaa caggacattc tctggtttca
     3961 ggtctcgatg aacaaccata tgcctatgac agtaatccac agcagacaga atctgctgaa
     4021 agagccgcct ggcttccatc tcttcaaccc gtccatgctt acagatgtag tcaaataatt
     4081 cacctccaga cacatattcc attaccataa aaaaatctgt tggagtgctg atcacctggt
     4141 atagtttgat aatatgagga tgacgaaaga gttttagatt ttgaatttct cgttttattt
     4201 ttccaacaac atctaaactg cgaatcttct gtctatttaa gattttaact gccactttat
     4261 ggcctgttaa ttgatgttct ccaatcttca ctttgccgaa ggtgccgacg cccagcgtgt
     4321 cgcccagcac gtagtgtccg atcttcaccc gcccgtcgtg cttctgcttc tcagccgaat
     4381 tccccgggag gctagcataa tcaggaacat catacatggt ggcggcaagc ttagatctcc
     4441 tccaaaaaag cctcttcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     4501 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     4561 taggggcggg atgggcg
//
