LOCUS       sequence-408633-        3291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor IAA28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-ZmIAA28
FEATURES             Location/Qualifiers
     source          1..3291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(848..947)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(964..983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(965..983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(988..1004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(988..1004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1106..1125)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1117..1926
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1184..1203)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2073..2661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2562..2581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2815..2832
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2991..3018)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3110..3196)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3245..3267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3259..3276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3260..3276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct tcaaaatgtc gcctccactc
      121 gagctcgact acataggcct ctcgccgccg gcggcagccg ccgccgctga aaacgacgaa
      181 ctgaagggta ctgagctccg cctcgggctg ccagggtccg ggtcgccgga ccgccgtgtc
      241 gtggctgcca ccgcgaccac cctggacctg ctcccggcca agggcgccaa gcgcgggttt
      301 tctgacgagg cgcccccgcc atcgcctgtt gccactgccg gaaagggaaa gaaggtggca
      361 gaagaggagt acgacgagaa gaaggtggca gcgaccccgc agccggccgc caaagcacag
      421 gtggtgggat ggccaccagt ctgcaactac cgcaagaaca cgatgactac cacccagctg
      481 gagggcagca aggaggatgg tgatgccaag cagggtcagg ggttcctgta cgtcaaggtc
      541 agcatggatg gcgcgccgta cctcaggaag atcgacctca agacttacaa gaactacaag
      601 gacctgtcga ccgcgcttga gaagatgttc agtggcttca gtactggcaa ggacggctca
      661 tctgagtacc gcaaggatgg tgaatatgtt ctgacttacg aggacaagga tggagattgg
      721 atgcttgtcg gcgacgtacc atgggagatg ttcgccggct cctgccgcag gctcaggatc
      781 atgaaaggct cagatgcgat tggactcgct ccaagggcag ccgataagtc caagaaccgc
      841 aactgacacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
      901 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
      961 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     1021 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     1081 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     1141 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1201 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     1261 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1321 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1381 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     1441 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1501 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1561 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1621 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     1681 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1741 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1801 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     1861 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     1921 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     1981 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2761 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2821 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2881 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2941 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     3001 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     3061 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     3121 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     3181 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     3241 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
