LOCUS       sequence-408635-       16936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16936)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 16936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1769
FEATURES             Location/Qualifiers
     source          1..16936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12793..13381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12846..12866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13264..13275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13453..13686
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13764..13885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13801..13820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13855..13874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13925..14060
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14080..14099)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14081..14099)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14109..14126)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14109..14125)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14118..14140)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14267..14722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14354..14373)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14564..14585
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        14748..14852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14853..14921,14922..15713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15071..15090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15884..16472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16373..16392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16626..16643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    16760..16781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16796..16801,16802..16819,16820..16826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16834..16850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16839..16861
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16858..16874
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16858..16874
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     16893..16913
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        16895..16913
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gagaaacagc agattatatt
     4141 ggctaatcaa gatggtggaa cagtggcagg agcagcacct accttctttg tcatcttaaa
     4201 gcagccagga aatggcaaaa ctgatcaagg aattttggtt actaatcagg atgcctgtgc
     4261 tttggctagt agtgtgtcat caccagtaaa atctaaaggg aagatttgcc ttccagctga
     4321 ttgtactgtg ggtggaatca ctgttaccct cgataacaat agtatgtgga atgagttcta
     4381 tcatcgaagc acagagatga ttctgaccaa gcaaggaaga cgcatgtttc cttactgtcg
     4441 ttattggata acaggtttag attcaaattt gaagtatatt cttgtcatgg atatatctcc
     4501 tgtggataac catcgttata agtggaatgg tcgttggtgg gaacctagtg ggaaggctga
     4561 acctcatgtt ttggggaggg ttttcattca tccagaatct ccttccacag gtcattattg
     4621 gatgcatcaa ccagtatctt tctataaact caaacttacc aacaatacac tggaccaaga
     4681 agggcatatc atcttgcact ctatgcatcg ttacctgccg aggcttcatt tggtgcctgc
     4741 agaaaaggct gtggaggtga tacaattaaa tggccctggt gtccacactt ttaccttccc
     4801 acagactgaa ttctttgcag taacagctta tcagaacatt cagattactc agctgaaaat
     4861 agattacaat ccatttgcca aaggctttcg ggatgatggg ctgaataata agccccagag
     4921 agatggaaaa caaaagaaca gctctgacca agaagggaat aatatttcca gttcttctgg
     4981 tcatcgggtc cgtcttacag aaggtcaggg gtcagagata caaccaggtg atttggatcc
     5041 tttgtcaagg ggtcatgaaa catcaggcaa gggtttggag aagacttccc ttaatataaa
     5101 acgagacttt cttggtttca tggatactga ttcagcactt agtgaagttc ctcaattgaa
     5161 gcaagagatt tctgaatgtc ttattgccag cagttttgaa gatgactccc gtgtagcctc
     5221 accgttagac cagaacggaa gcttcaatgt tgttattaaa gaggaacctc tagatgatta
     5281 tgactacgaa cttggtgagt gcccagaagg ggtcactgtg aaacaggaag agacagatga
     5341 agagacggat gtatactcaa acagtgatga tgatcctata ctagagaaac agctaaagag
     5401 gcacaataaa gttgacaacc cagaagctga ccatctatct tctaaatggc ttccaagcag
     5461 cccatcaggt gttgctaaag ctaaaatgtt caaattagac actggaaaga tgccagtagt
     5521 ctatctggag ccctgtgctg tcaccagaag cacagttaag atttctgaac tccccgataa
     5581 catgctttcc acatctcgaa aggataaatc ttctatgttg gcagaattgg aatatttgcc
     5641 tacatacatt gaaaattcca atgagactgc cttctgctta ggcaaggaat cagaaaatgg
     5701 tcttagaaaa cattcaccag atctcagagt ggtacaaaaa tatcccttac tgaaagagcc
     5761 tcagtggaaa tatcctgata tatctgacag cattagcaca gaaagaatac tcgacgattc
     5821 aaaggattca gttggagact cactttcagg aaaagaggac ttgggcagaa agagaacaac
     5881 tatgcttaag attgcaacag ccgcaaaggt agtgaatgct aatcagaatg cctctccaaa
     5941 tgtccctgga aaaagaggaa ggccacgaaa attgaaactc tgtaaggcag gacgaccacc
     6001 taagaacaca ggaaagtctt taatttctac aaagaataca cctgtaagcc ctgggagtac
     6061 ctttccagat gtgaagcctg atctggaaga tgtggatggt gttctctttg tttcctttga
     6121 atcaaaggaa gctctagaca ttcatgcagt tgatgggaca acagaagaat cttctagtct
     6181 ccaggcatca accacaaatg actcaggtta cagagcaaga atttcccagt tggaaaagga
     6241 attgatagaa gatttgaaga ctttgcggca caagcaggtg atacatcctg gtcttcaaga
     6301 agtgggctta aaattgaatt cagtggatcc aacaatgagc attgatctta aatacttggg
     6361 agtacagtta cctttggctc cagctactag ctttcctttt tggaacctta caggaaccaa
     6421 ccctgcctct cctgatgcgg gatttccctt tgtttctagg acagggaaaa ccaatgattt
     6481 cactaagatc aagggatgga ggggaaaatt tcatagtgct tctgcatcta ggaatgaagg
     6541 tggaaattca gaaagttcac tgaaaaatcg ttctgctttc tgtagtgata agctagatga
     6601 atacttggaa aatgaaggca agctgatgga aacaagcatg ggtttttctt ctaatgctcc
     6661 cacatctcct gtggtgtacc agcttcccac taagagtacc agttatgtac gaacacttga
     6721 tagtgtacta aagaagcaat ctactatttc cccttctacc tcttattctt tgaaacctca
     6781 ttctgtaccc cctgtctctc gaaaggcaaa gtctcaaaac agacaggcaa ctttcagtgg
     6841 ccgaactaaa tcatcttata aatccatttt accataccct gtttcaccaa agcagaaata
     6901 ctctcatgtg attctaggag ataaggttac caagaattct tcaggcatca tctcagaaaa
     6961 tcaggcgaat aactttgttg tgccaacttt ggatgaaaat atatttccaa agcagattag
     7021 tttgcggcag gcacagcagc agcagcaaca gcaacaggga agtcgccctc caggcttgtc
     7081 taaatctcag gtgaagctaa tggacctgga agactgtgca ctttgggaag gaaaaccaag
     7141 gacatacatc acagaagagc gagcagatgt atccttaaca actctactta cagctcaagc
     7201 atccctcaaa actaaaccta tccacacaat cataaggaaa cgagcccctc cctgcaacaa
     7261 tgacttctgt cgactgggtt gtgtatgttc cagtctagct ttggagaagc gccaacctgc
     7321 tcactgccgc cgaccagact gcatgtttgg ttgtacttgt ttgaaaagaa aagttgtact
     7381 tgttaaagga ggatccaaaa ctaagcattt tcagaggaag gctgctcatc gagatccagt
     7441 attttatgat actctgggag aggaggcaag ggaggaggaa gaaggaatca gggaggagga
     7501 ggaacaattg aaagagaaaa agaagagaaa gaagctagaa tacactatat gtgagacaga
     7561 gcctgaacag cctgttcgac attacccatt atgggtaaaa gtagaaggtg aagtagatcc
     7621 agaaccagtt tatatcccca cgccttctgt cattgagcct atgaaaccat tgttattgcc
     7681 tcagccagaa gttttatctc ctactgtgaa gggcaaactg ctcactggaa ttaaatctcc
     7741 acggtcatat actcccaaac ccaatcctgt gattcgggaa gaggacaaag atccagtcta
     7801 cttgtacttt gaaagtatga tgacttgtgc tcgagttcga gtatatgagc gaaaaaaaga
     7861 ggaccagaga caaccatctt cctcctcctc cccatctcca tcatttcagc agcaaacttc
     7921 atgtcattct agccctgaga accataataa tgcaaaggaa cctgattctg aacagcagcc
     7981 cttaaaacaa ctcacctgtg acttggagga tgattctgat aaattacaag aaaagagctg
     8041 gaagtcttcc tgcaatgaag gagaatcctc ttctacttct tatatgcatc agaggtcacc
     8101 tggtggtccc accaaactga ttgagatcat ctcagactgc aactgggagg aagatcggaa
     8161 caagattttg agcatcttat cccagcacat caatagcaac atgccacaat cacttaaggt
     8221 gggcagcttc atcattgagt tggcttctca gcgaaagagc cggggtgaga agaaccctcc
     8281 tgtttattct tctcgtgtga aaatctctat gccatcatgt caagaccaag atgatatggc
     8341 tgagaaatct ggatcagaga ctcctgatgg tccattgtcc cctgggaaaa tggaggatat
     8401 ctctcctgtg cagacagatg ccctggattc agtgagggag agattacatg gaggcaaagg
     8461 tctgcctttt tatgcagggc tttctcctgc agggaagctt gtggcctata aacgtaaacc
     8521 cagttcaagt acatctgggc ttatccaggt agcatccaat gccaaggtgg ctgcatccag
     8581 gaaaccacgt accctgttgc cttcaacatc caattccaaa atggcatcct cctctggcac
     8641 tgcaacaaat cgccctggga agaatctgaa ggcgtttgtc ccagcaaaac ggccaattga
     8701 aaatgctgct caaattccag tggctactcc acaggtctct cctaacacag tgaaacgtgc
     8761 tggacctcga ttgttgttga ttccagtgca gcagggttct cctactctta gacctgtctc
     8821 aaacacacaa cttcagggac atcggatggt cttgcagcct gttaggagtc caagtggaat
     8881 gaacttattc aggcacccta atgggcagat tgtccagctt ctacctttgc atcagcttcg
     8941 aggctctaat acccagccca acttacagcc tgtcatgttt cggaacccag ggtctgtgat
     9001 gggaatccgg ttacctgctc cttccaaacc ctctgagact ccgccatctt ccacttcgtc
     9061 ctctgctttc tctgtcatga atcctgtaat tcaagctgtt gggtcttctt cagcagtgaa
     9121 tgttatcact caggcaccat cattgctttc ctctggagct agttttgtgt ctcaggctgg
     9181 tacattgacc ctgaggattt ctcctcctga accacaaagc tttgcaagta aaacaggctc
     9241 tgaaaccaaa ataacttata gctcaggagg acagcctgtt ggtacagcca gtcttattcc
     9301 tctccagtct ggtagttttg ccttgttaca gctcccagga caaaagcctg ttcctagctc
     9361 cattcttcag catgttgctt cccttcagat gaagagagaa tctcagaatc cagaccagaa
     9421 agatgaaaca aactcaataa aaagagagca agaaacgaag aaggttctac agtcagaagg
     9481 agaggctgta gaccctgagg ctaatgtaat aaaacaaaac tcaggagctg ctacctcaga
     9541 agaaactctg aatgattcct tggaagatag gggtgatcat ttggatgaag aatgccttcc
     9601 agaagaaggt tgtgcaactg tcaaaccatc tgagcattcc tgtatcactg ggtcacatac
     9661 agatcaagat tataaagatg ttaatgaaga atatggggct aggaatcgta agagttccaa
     9721 agaaaaagtg gctgttctgg aagttaggac catttctgaa aaagccagta ataagacagt
     9781 ccaaaattta agtaaagtac agcatcaaaa acttggtgat gtgaaggtgg aacagcagaa
     9841 aggatttgac aatccagaag aaaactcaag tgaatttcca gtcaccttta aggaagaaag
     9901 taaatttgaa ttgtcaggaa gcaaagttat ggagcagcaa tctaatctac agccagaggc
     9961 caaagagaag gaatgtggag actctctgga gaaagacagg gaaagatgga gaaaacatct
    10021 gaagggcccc ttaaccagga aatgtgttgg agcttcacag gaatgtaaga aagaggcaga
    10081 cgagcagtta attaaagaaa caaagacatg tcaggaaaat tcagatgtgt ttcagcaaga
    10141 acaaggcatc tctgacttac ttggaaaaag tggaattact gaagatgcca gagttttgaa
    10201 aactgaatgt gattcttgga gtaggatttc taatccttca gccttctcca ttgttcctag
    10261 gagagctgca aaaagcagca gagggaatgg acattttcag ggtcacttac tgctacctgg
    10321 agaacagata caaccaaagc aagagaagaa gggtgggaga agcagtgctg acttcactgt
    10381 tttggatttg gaagaagatg atgaagatga taatgagaaa actgatgatt ctattgatga
    10441 aattgtggat gttgtttctg actaccagag tgaggaggtt gatgatgtag aaaagaataa
    10501 ctgtgtagaa tacattgagg atgatgagga gcacgtggac attgagactg tagaagagct
    10561 ctcagaggaa attaatgttg ctcacctgaa gaccacagcg gcccacacac agtcattcaa
    10621 acagccgtcc tgtactcaca tctctgcaga tgaaaaagca gctgaaagga gtcgaaaggc
    10681 tccaccaatt cctctaaaac tgaagcctga ttactggagt gacaaactac agaaagaagc
    10741 agaagcgttt gcttattatc gccggacaca cactgccaat gagcggcggc ggcgtggtga
    10801 aatgagggat ctctttgaga aattaaagat cacattggga ttacttcatt cttccaaggt
    10861 ttccaaaagt ctcattctta ctcgagcctt cagtgaaatt cagggactaa cagatcaggc
    10921 agacaaattg ataggacaga aaaatctcct gactcgaaaa cggaatattc tgatacggaa
    10981 agtatcgtct ctttcaggta agacagaaga agtggtcctg aagaagctag agtatattta
    11041 tgcaaaacag caagcactag aggcacaaaa aagaaaaaag aagatgggat cagatgagtt
    11101 tgacatatct cccagaatta gcaaacagca ggaaggatct tctgcatcat ctgtagatct
    11161 tggacagatg tttataaata acaggagggg gaaacctttg attctttcca gaaaaaaaga
    11221 ccaggccaca gaaaatacct cacccttgaa cactccacac acctctgcca accttgtgat
    11281 gactccgcaa gggcaattgc tcaccctaaa aggtccccta ttctcaggac cagtggtagc
    11341 tgtttctcct gatctcttag aatctgatct taagcctcaa gttgccggta gtgctgtggc
    11401 tctaccagaa aatgacgact tatttatgat gccacgaatt gttaatgtga catcattggc
    11461 cacagaggga ggtttggtag atatgggtgg cagcaaatat cctcatgaag ttcctgatag
    11521 caagccatct gaccatctga aagacaccgt caggaatgaa gataattcct tagaggataa
    11581 gggtagaatc tcttccagag gaaacagaga tggcagagtg acgttgggtc caacgcaggt
    11641 ttttctggca aacaaagatt ctggttatcc acaaatagtt gacgtttcca atatgcagaa
    11701 agcacaagag ttcttaccta aaaagatttc tggtgatatg agagggattc agtataaatg
    11761 gaaagagagt gaatcaagag gggagagagt gaagtcaaag gattcttcat ttcataaatt
    11821 aaagatgaaa gatctcaagg actcaagcat agagatggaa ctgaggaaag taacatcagc
    11881 tatagaggaa gcagctcttg attccagtga actgctgact aacatggaag atgaggatga
    11941 tactgatgag acactgactt cactgctcaa tgaaattgcc tttcttaatc aacaactaaa
    12001 tgatgactct gttggcctgg ctgaactacc cagctctatg gatacagagt tcccagggga
    12061 tgctcggcgg gcttttatta gtaaggttcc tcctggaagc agagcaactt tccaggttga
    12121 gcacttggga actggtttga aagagttgcc tgatgttcaa ggggagagtg actctatcag
    12181 tcccctcctc ttgcacttgg aagacgatga cttttctgag aatgaaaaac aacttgcaga
    12241 accagcctct gagccagatg tccttaagat tgttattgac tctgaaataa aggattccct
    12301 cctttccaac aagaaagcta ttgatggagg gaagaatact tctggcctcc ctgcagagcc
    12361 cgaaagtgtg tcctcacccc ccaccctaca catgaagact ggcttggaga acagcaacag
    12421 cacagacact ttgtggaggc ctatgccaaa gttggcccct ctaggtttaa aagtagctaa
    12481 tccttccagt gatgcagatg gtcagagtct caaggtgatg ccttgtttgg cacctatagc
    12541 tgccaaagtt gggtcagttg gacacaaaat gaacttaaca gggaatgacc aggaaggccg
    12601 ggaaagcaag gtgatgccta cattggcacc tgttgtggct aaattgggca actcgggggc
    12661 ctcaccaagt tctgcaggga aatagtaatg aactagtccg taacttgaaa gtatttcgat
    12721 ttcttggctt tatatatctt gtggaaagga cgaacgccgt taatagcagg atttacaacg
    12781 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    12841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    12901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    12961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    13021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    13081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    13141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    13201 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    13261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    13321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    13381 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    13441 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
    13501 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    13561 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    13621 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    13681 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
    13741 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
    13801 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13861 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
    13921 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    13981 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    14041 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
    14101 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
    14161 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
    14221 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
    14281 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
    14341 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
    14401 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    14461 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
    14521 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    14581 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
    14641 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    14701 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    14761 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    14821 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    14881 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    14941 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    15001 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    15061 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    15121 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    15181 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    15241 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    15301 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    15361 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    15421 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    15481 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    15541 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    15601 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    15661 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    15721 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    15781 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    15841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    15901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    15961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    16021 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    16081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    16141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    16201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    16261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    16321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    16381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    16441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    16501 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    16561 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    16621 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    16681 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    16741 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    16801 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
    16861 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
    16921 agctggagct gcaagc
//
