LOCUS       sequence-408653-       12444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GEMS 2.0 sensor system with PEST destabilization domain 
            (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) 
            (pFM577+PEST).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Marayati BF, Thompson MG, Holley CL, Horner SM, Meyer KD
  TITLE     Programmable protein expression using a genetically encoded m(6)A 
            sensor.
  JOURNAL   Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3.
  PUBMED    38168988
REFERENCE   2  (bases 1 to 12444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3."
COMMENT     Sequence Label: pGEMS-II-PEST
FEATURES             Location/Qualifiers
     source          1..12444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             629..655
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             629..655
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    697..808
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1786..2296
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2215..2233
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2314..2991
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(2494..2514)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2712..2731
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2906..2929
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             3015..3195
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3227..3451
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3497..3925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3584..3603)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3794..3815
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3934..3954)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3939..4268
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4119..4254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4181..4200
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4316..4363
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4382..4756
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4886..5007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4923..4942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4977..4996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5056..5072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5056..5072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5069..5091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5080..5096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5104..5110,5111..5128,5129..5134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5149..5170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5287..5304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5458..6046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5538..5557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6217..7008,7009..7077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6840..6859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7078..7182)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7257..7276)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7448..7827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7828..8031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7982..8002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8099..8108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9038..9514
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    9659..9776
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9883..9901,9902..9924,9925..9943,9944..9966,9967..9985,
                     9986..10008,10009..10027,10028..10050,10051..10069,
                     10070..10092,10093..10111,10112..10134,10135..10153)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10409..11587
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10640..11578
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11535..11555
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        11604..11622
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        11718..11736
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11750..12436
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 cgagactccc gggacctcag agtccgccac accagaaccc cacccagtgt tggagaagct
       61 tcggtccatt aataactata accccaaaga ttttgactgg aatctgaaac atggccgggt
      121 tttcatcatt aagagctact ctgaggacga tattcaccgt tccattaagt ataatatttg
      181 gtgcagcaca gagcatggta acaagagact ggatgctgct tatcgttcca tgaacgggaa
      241 aggccccgtt tacttacttt tcagtgtcaa cggcagtgga cacttctgtg gcgtggcaga
      301 aatgaaatct gctgtggact acaacacatg tgcaggtgtg tggtcccagg acaaatggaa
      361 gggtcgtttt gatgtcaggt ggatttttgt gaaggacgtt cccaatagcc aactgcgaca
      421 cattcgccta gagaacaacg agaataaacc agtgaccaac tctagggaca ctcaggaagt
      481 gcctctggaa aaggctaagc aggtgttgaa aattatagcc agctacaagc acaccacttc
      541 catttttgat gacttctcac actatgagaa acgccaagag gaagaagaaa gtgttaaaaa
      601 ggaacgtcaa ggtcgtggga aactcgagta cccctacgac gtgcccgact acgcctgagt
      661 ttaaacccga aacttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      721 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      781 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      841 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      901 ggctcgaggt taacgaattc taccttaccc agagtgcagg tgtgtggaga tccctcctgc
      961 cttgacattg agcagcctta gagggtgggg gaggctcagg ggtcaggtct ctgttcctgc
     1021 ttattgggga gttcctggcc tggcccttct atgtctcccc aggtacccca gtttttctgg
     1081 gttcacccag agtgcagatg cttgaggagg tgggaaggga ctatttgggg gtgtctggct
     1141 caggtgccat gcctcactgg ggctggttgg cacctgcatt tcctgggagt ggggctgtct
     1201 cagggtagct gggcacggtg ttcccttgag tgggggtgta gtgagtgttc ctagctgcca
     1261 cgcctttgcc ttcacctatg ggatcgtggc tgtcagttaa ttaaccttcc gcgggagctc
     1321 acggggagag ccccccgcca aagcccccag ggatgtaatt gcatccctct tccgctaggg
     1381 ggcagcagcg agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc
     1441 cggagccggc agcgtgcggg gacagcccgg cacggggaag gtggcacgcg atcgctttcc
     1501 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaaag
     1561 ctttaggctg aaagagagat ttagaatgac agaatcatag aatggcctgg gttgcaaagg
     1621 agcacagtgc tcacccagct ccaaccccct gctatgtgca gggtcgccaa ccagcagccc
     1681 aggctgccca gagccacatc cagcctggcc ttgaatgcct gcagggatgg ggcatccaca
     1741 gcctccttgg gcaacctgtt cagtgcgtca cggatccaat tccacggggt tggggttgcg
     1801 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     1861 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     1921 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     1981 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     2041 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     2101 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     2161 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     2221 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     2281 cgacctctct ccccagctgt agctagcaca accatggata gcactgagaa cgtcatcaag
     2341 cccttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2401 gagggcgagg gcgagggcaa gccctacgag ggcacccaga ccgccaagct gcaggtgacc
     2461 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     2521 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     2581 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2641 gactcctccc tgcaggacgg caccttcatc taccacgtga agttcatcgg cgtgaacttc
     2701 ccctccgacg gccccgtaat gcagaagaag actctgggct gggagccctc caccgagcgc
     2761 ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggcgctgaa gctgaagggc
     2821 ggcggccact acctggtgga gttcaagtca atctacatgg ccaagaagcc cgtgaagctg
     2881 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
     2941 gtggtggagc agtacgagcg cgccgaggcc cgccaccacc tgttccagta gggctagctg
     3001 gtccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3061 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3121 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3181 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3241 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3301 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3361 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3421 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3481 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3901 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4021 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4201 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4261 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4321 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     4381 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     4441 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     4501 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     4561 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     4621 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     4681 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     4741 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     4801 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4861 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4981 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5041 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5101 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5161 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7201 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     7261 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7321 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7381 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     7441 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7501 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     7561 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     7621 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     7681 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     7741 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     7801 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     7861 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     7921 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     7981 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     8041 aaccgtcaga tccgctagag atccgcggcc gcgctagcgt ttaaacgggc cctctagagc
     8101 cgccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     8161 ggatggcgat gtaaatggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     8221 ctacggcaag ctcaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     8281 caccctcgtc accaccctca cctacggcgt gcagtgcttc agccgctacc ccgatcacat
     8341 gaagcagcac gatttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     8401 cttcttcaag gatgatggca attaccgtac ccgcgccgag gtgaagttcg agggcgatac
     8461 cctggtgaat cgcatcgagc tgaagggcat cgatttcaag gaggatggca atatcctggg
     8521 gcacaagctg gagtacaatt acaatagcca caatgtctat atcatggccg ataagcagaa
     8581 gaatggcatc aaggtgaatt tcaagatccg ccacaatatc gaggatggca gcgtgcagct
     8641 cgccgatcac taccagcaga atacccccat cggcgatggc cccgtgctgc tgcccgataa
     8701 tcactacctg agcacccagt ccgccctgag caaagatccc aatgagaagc gcgatcacat
     8761 ggtcctgctg gagttcgtca ccgccgccgg gatcactctc ggcatggatg agctgtacaa
     8821 gttgcttagc catggcttcc cgccggaggt ggaggagcag gatgatggca cgctgcccat
     8881 gtcttgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctt ctgctaggat
     8941 caatgtgtta gatgcggcgg acttacgaca gttgcgttac accctttctc gacaaaacct
     9001 aacttgcgca gaaaacatgc caatctcatc ttggcttatc agtctgattg cggcgttagc
     9061 ggtagatcac gttatcggca tggaaaccgt catgccgtgg aacctgcctg ccgatctcgc
     9121 ctggtttaaa cgcaacacct taaataaacc cgtgattatg ggccgccata cctgggaatc
     9181 aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc agcagtcaac cgagtacgga
     9241 cgatcgcgta acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg gtgacgtacc
     9301 agaaatcatg gttattggcg gcggtcgcgt ttatgaacag ttcttgccaa aagcgcaaaa
     9361 actgtatctg acgcatatcg acgcagaagt ggaaggcgac acccatttcc cggattacga
     9421 gccggatgac tgggaatcgg tattcagcga attccacgat gctgatgcgc agaactctca
     9481 cagctattgc tttgagattc tggagcggcg ataagcctca ttgtgcattc tctcggacag
     9541 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     9601 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     9661 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9721 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9781 tttattacag ggacagcaga gatccagttt ggttaccagt gtgatggata tctgcagaat
     9841 tcgcccttgg atccgaattc ctgcagcccc gactttcact tttctctatc actgataggg
     9901 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
     9961 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
    10021 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
    10081 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
    10141 tcactgatag ggagtggtaa actcgagggg gatccactag catgaagggc gaattccagc
    10201 acactggtaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg gcgcctcccg
    10261 cgggcgcccc cctcctcacg gcgagccgcg ttgacattga ttattgacta ggcttttgca
    10321 aaaagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10381 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
    10441 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
    10501 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
    10561 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
    10621 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
    10681 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
    10741 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
    10801 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
    10861 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
    10921 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
    10981 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
    11041 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
    11101 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
    11161 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
    11221 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
    11281 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
    11341 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
    11401 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
    11461 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
    11521 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
    11581 gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca agctggctag
    11641 gtaagcttgg taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc
    11701 agcacagtgg cggccgctaa tacgactcac tatagggaga gccgccacca tgagctcaga
    11761 gactggccca gtggctgtgg accccacatt gagacggcgg atcgagcccc atgagtttga
    11821 ggtattcttc gatccgagag agctccgcaa ggagacctgc ctgctttacg aaattaattg
    11881 ggggggccgg cactccattt ggcgacatac atcacagaac actaacaagc acgtcgaagt
    11941 caacttcatc gagaagttca cgacagaaag atatttctgt ccgaacacaa ggtgcagcat
    12001 tacctggttt ctcagctgga gcccatgcgg cgaatgtagt agggccatca ctgaattcct
    12061 gtcaaggtat ccccacgtca ctctgtttat ttacatcgca aggctgtacc accacgctga
    12121 cccccgcaat cgacaaggcc tgcgggattt gatctcttca ggtgtgacta tccaaattat
    12181 gactgagcag gagtcaggat actgctggag aaactttgtg aattatagcc cgagtaatga
    12241 agcccactgg cctaggtatc cccatctgtg ggtacgactg tacgttcttg aactgtactg
    12301 catcatactg ggcctgcctc cttgtctcaa cattctgaga aggaagcagc cacagctgac
    12361 attctttacc atcgctcttc agtcttgtca ttaccagcga ctgcccccac acattctctg
    12421 ggccaccggg ttgaaaagcg gcag
//
