LOCUS       sequence-408662-       12673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of the centrosomal protein Akna fused to 
            mOrange2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2
FEATURES             Location/Qualifiers
     source          1..12673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3283..3303)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3306..3315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4424..4457
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4500..4509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6789..6800
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             8775..9485
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9592..9609)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9598..9822
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9894..9911)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9894..9910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9903..9925)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10051..10506)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10188..10209)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10400..10419
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10533..10637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10638..10706,10707..11498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10856..10875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11669..12257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12158..12177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12411..12428
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12545..12566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12581..12586,12587..12604,12605..12611)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12619..12635
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12624..12646
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12643..12659
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12643..12659
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgrtgggg gsgggggggg ggggggggsg sgsgssrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggsggr grggkgsggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggkggcgg crggkggggg kgcsgggcgg
     1261 ggcggggccg ccksgggssg gggrgggcks gggggrgggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tcttcagtcc ccatcctcga gcagcctcac
     2281 catggcccca gtctcagagt ccaggtttct gatgtagttc atcacaatgt tcacattggt
     2341 ccagccacca gcctgcatga tttcagggat ggacacacca gccctggcca tgtccctggc
     2401 agcacccact ctggcagagt ggccagacca ggccaggtat ctctgcccag agtcatcctt
     2461 ggcaccatag atcaggcggt gggtggcctc aaagatccct tccagggccc gggtggacag
     2521 ttgggaggtg gcagaagggg cagccacacc attctttctg acccggcaga acaggtagtt
     2581 gttggggtca tcagccacac cagacacaga gatccatctc tccaccagct tggtaacccc
     2641 cagggacagg gccttctcca caccagctgt ggacaccagg gtcttggtcc tgccaatgtg
     2701 gatcagcatt ctcccaccat cggtgcggga gatgtccttc actctgattc tggcaatttc
     2761 ggcaatgcgc agcagggtgt tgtaggcaat gcccaggaag gccaggttcc tgatgtcctg
     2821 gcatctgtca gagttctcca tcagggatct gacttggtca aagtcagtgc gttcaaaggc
     2881 cagggcctgc ttggctctct ccccagcatc cacattctcc tttctgattc tcctcatcac
     2941 cagggacaca gcattggagt cagaagggcg aggcaggcca gatctcctgt gcagcatgtt
     3001 gagctggccc aggtgctgtt ggatggtctt cacagccagg cctctggctt gcaggtacag
     3061 gaggtagtcc ctcacatcct caggttcagc agggaaccat ttcctgttgt tcagcttgca
     3121 ccaggcagcc caggatctgc acacagacag gagcatcttc caggtgtgtt cagagaaggc
     3181 ctgcctgtcc ctgaacatgt ccatcaggtt cttcctgact tcatcagagg tggcatccac
     3241 agggagggca ggcaggtttt ggtgcacagt cagcaggttg gagactttcc tcttcttctt
     3301 gggcaccatg gtggcgaatt cgttgctgct tcgcggctgc cgggtggatg gcggaggcga
     3361 ggaggaggtg ttgcttctta cagcgcgact cttaagcggt cgatgtaaga gaacctgcgg
     3421 cacttgggcg gatgcgcctc cttatataca gctcggtcac catggggatg ggccaaggcc
     3481 ttttcctgtt ggtccagacc cctccgtctg cccagagaag ccgcgacagg agagtggtga
     3541 ttggtgcaag cgacatggta tgaggtaatc tctcccccct tccccccctc gctagcatcc
     3601 gtagattttg catccttcag acaaaggcct cgcaaaagca actagccgag catccattat
     3661 ggggtggagc tggggagggg tagagaggga agaaagaaaa cgggggttgg gtgattatac
     3721 ggagcttaaa ccttaaactg tagttaggat aatttaaggt tttccccaac acttaatcac
     3781 agaatttgtg ttgagattct ttatggtaaa ttccagcttt tcatttatac tacactaaat
     3841 ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt taaataaagt agcaagagct
     3901 atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt tttacagttg tattattgtg
     3961 gttattatta ttactattat tattaatatt tgagcaaaat tcagaagctc agtatgaata
     4021 gaccacgcct atgaccctca acagcacccg ggaggcagag gcaggaagac ttgcagctca
     4081 aggtcatcat aaccgccctt agtggtttat gcctgtaaat cccagcattg ggaagcggag
     4141 gactgcggtt attttgtacc cggcctggat atatagtgtg atatcctgtc tcaaaaacca
     4201 aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga gaaacaatat acaaagtagt
     4261 acttaacgct ttactagaga gcttgctttc tttagcggta gtgctctccc atcacccctc
     4321 cccgctcgcc cccttacccg accctacctc accccagagc ctgtgttatt ttgtccctag
     4381 aaatgagcca tccctcctaa tacggaattc cggaaccctt aatataactt cgtataatgt
     4441 atgctatacg aagttattag gtccctcgac ctgcagcctc cgcagccccc aagctagcag
     4501 ccaccatggc cagctcaggg gcaaaggcac aatgggttgg gccaagcctg gggcaagggc
     4561 cccgaaggcg gcgctgggct tgggctgagg aacaagatac agatggaaga agagaccagg
     4621 gctggggaaa cagccagtct cttcccgaag cccccagtcc tgagctccta gaggacttcc
     4681 gtcgggcaca ggagcacctg ccacccctgg agtgggaccc agacatgcag gactccgaag
     4741 agtcttctgg agaagagacc gaagctgacg atgcaagcag cccagagggt tccaccgtgc
     4801 ctctgccctg gctctccagg agcaaccaac agctggacat gtctgaagag gagctggatg
     4861 aggcctctgg gaggcctgag gtagacttag ctggagagag ttgtacggag cttgagtgtg
     4921 aagaccaggg agattccagc cctccacctc caggacaggg gccagccaaa ggctgggtca
     4981 cctttatcaa acaaggcagt aactacagac cttcagagca tctcgaggcc cagccatctg
     5041 ttgaacatag ccgcacaaag tcttggagca gtggaactgt gagcctccgt caacctagtg
     5101 acagccttgg ttctacctgg gaaggagata ccgaagtccc tcagccttcc atcttgccca
     5161 aagccctgcc acagagtccg tgccacaact ttccacaccc aggtgacaga aatggaggtg
     5221 acgttgctcc ggcaacaccc acagaattcc gggactcctt agcagcgcca gcccagaatg
     5281 ccgagtgctc tgcaggcacg tggggggaag aaaccaccag cctacccagc tctcggcctg
     5341 aggaccaaac ctggaagagg actaagacat cacctaagcc actgccctcc cggtttactg
     5401 gttctgtcag ccccctgagt actcggcttg gggcaataaa gaaagttgtg ccccagcaca
     5461 agcagggagc cactctggct ggtcactctt cttcacaagc tcccaagtat ggcagggggc
     5521 gccgcctgaa ctacccactc cccgacttct ccaaggtggg gccccgggtg aggtttccca
     5581 aagatgagaa ctaccgaccc ccgaagtcca gaggccacaa caggcaacaa ggctctacca
     5641 gacccctcat cttcaagtcg ccagctgaga ttgttcggga tgtactgttg agtagtggag
     5701 aggcgtccct ggccaaggaa tcttctcttg ctcacaccat caccagggtg ccccaggagt
     5761 tccagacacc cgaacaagcc actgagctag ttcatcagct ccaggaggac tatcacaagc
     5821 tcctcaccaa gtatgcggag gctgagaaca ctatcgacca acttcgcctt ggggccaagg
     5881 tgcacctgta ttcagaccca ccacagccca gtcagagctt ctgctcgggg tcaatgccac
     5941 aaggaagcaa ggtcttatcc ttctctatcc cgcaacccag agtagcagag tggtggcctg
     6001 accctgccca ggatccacag gcctctgagg ccacagggtg gccgttccca cgaacagacc
     6061 tgagtccctc ctcgtccccc ggcgtggcca ctccagggcg gcttcctcag agccagggca
     6121 ttgccacaga ccagccctcc acaggacaga cccaggcact gacctctcag gccagccggc
     6181 tcctggccaa ggtgcagtcc ttcgaggaac tggtactggc tggacacctc ccacctcagg
     6241 accaaatcaa gagcttggag caactgaggg cagcccacat ggccctagag gcagagtacc
     6301 tgcaggcctg cagagaagag ctcctggacc ctcagcttga tgcctctcag gggagtccca
     6361 ggaccctcaa cctctgcagg gagctagaag cagagatata ccacctggga cagcgcctgg
     6421 aagagctaca ggaccatatg gaccagaccc aaagagagac agagccatgt aggccagacc
     6481 tgcaagacag caccccaacc atgtcctttc tgccccagtc agctcatctg tccatgcctt
     6541 caggacctgt ctccttgcca gatggccaaa cctaccagga gcctgccacc accaccacca
     6601 cctcccctgg aagctcttgc acgctcccca tcaacaagaa gttaagcctg agcatcaaaa
     6661 ctgaggaaag cccaagaggt ctcccggtcc cactcaggga caggacgctt caggtggagc
     6721 aggacttcca tggccttctg gagagatacc tcagcgttaa atccctccct gaagccttga
     6781 gggatgaaga tgaggatgac ctggaggagg aggaagagga gcaggaccat caaggcccct
     6841 tggaagttga tagtccagcc acagctccag ggaaaacaga agccgtgagg gtaccaccag
     6901 gagagcgccc aacacaggct gaggaaagcc atagggatgc tacccaggag gatgaggaac
     6961 agatgggacc tatgaagtca ccagacttcc ggccatccat ggccagagat acgtacacac
     7021 cagtcctgga tacagctgaa gtggctcaac ggggcaccaa agccatggtg tcacatcaga
     7081 gcagtctgac cagcctggaa gagagcaggc cctcagagct ccttcctcga aaagccctgc
     7141 ttcgggctgg tgggccccac actgaggagc cctggatggt gtcaccagag acagacagtg
     7201 gcttcgtggg ctcagaaacc agtatagtgt cacccttcac ccagacccca gagcaccggc
     7261 tctcccatgt cagcacttca ggaccatcag ctcaacacct cactgcctct gtgcctggtg
     7321 atagaacctc ccatcccaag gccaggggtc tcatggttcc cagaagagcc actgagactg
     7381 gcatacccag aagccgaacc cagcagcatt tctccagcct gagctctccc gggagagggg
     7441 cacagagctg ccacctagag gagacatcag tggctaaaat agcggttccc aggtctgagt
     7501 tcaagagaca gaagcagatt tccaaacagc tccttcccag tggaagaacc agccccgact
     7561 ctgccccagc tcccacagct gcctccacgc cccatggatc agcagagagc actgccaacc
     7621 tcctcctcaa caggacagag cgcgaccagg ccatcaagga tctgcaggcg gaggtgtcac
     7681 ggctccgcct tcaactggag gacagcctgc accggccaca cccagacggc ccagcctgtg
     7741 tagcttctgc tttcaaccac tccacccaga cccaggaaaa gctgggttcc tcaccctcct
     7801 ggggccccca ctatggcagt aaatccacag agaggctgtc tagggagcct aatggtgtag
     7861 agccagctga gccaatggga agacgtcgag ccaggtcctc ttcggtgcct cgggatgtac
     7921 ccagactgta cctaagttct gaatcggagt cgcctgcccc tcggctctca tctgagaaga
     7981 gcaggacctt tgaggagcac cccgaggctg cacagtgggg gacaagacca cagagcagct
     8041 cgaagcggag ggaaagagtt tccttccggg gtcaatacac aggccaggag taccacattc
     8101 tatccccaaa ggccatcctg aaggacagtg gcacaccgtc ctgtccccac tgccatccca
     8161 ttaggaccca ggacacaggc agtgctgtct ctagagacac cacacggggc tcatctgccg
     8221 ctgacaccct ccgctgtgcc ctgtgtggtg aagtcaagtc ctctgcagaa gcagatggct
     8281 ccagctcagg tccctctgag aagaacaccc ccaaaaagcc ttccaccccc atcctcaaac
     8341 ggaagaacag gcagacgggc tcgccagtac gaatggcccc tggactctgg tacctggctg
     8401 ctgcgccccc agccccagcc ccaccggctt tggcctacat ctcctcagct cccattatgc
     8461 cttatctacc acccactgtg tactatgcag ccccagcacc tacctcagcc cagacagctt
     8521 ccccgcagcc tgctcgggga cctcggagaa cccggcactc agtccaactg ggtctgaatg
     8581 acctggagga gctgcaggcc gcactcagag aggctgcaca ggccgccgag aacgttcgct
     8641 ccaccacccg ccagctgagc cgctccctgt ctgcggacct gcgccatgcc cgtagcctgc
     8701 gaggctcctg ccttttcgtc gacggcagca agcttggtgg aggcagcgca tcaggcggaa
     8761 gcggaagcag atctatggtg agcaagggcg aggagaataa catggccatc atcaaggagt
     8821 tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     8881 gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag gtgaccaagg
     8941 gtggccccct gcccttcgcc tgggacatcc tgtcccctca tttcacctac ggctccaagg
     9001 cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc cccgagggct
     9061 tcaagtggga gcgcgtgatg aactacgagg acggcggcgt ggtgaccgtg acccaggact
     9121 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     9181 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     9241 accccgagga cggtgccctg aagggcaaga tcaagatgag gctgaagctg aaggacggcg
     9301 gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     9361 gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     9421 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     9481 agtaagcggc cgccatatgc atcctaggcc tattaatatt ccggagtata cgtagccggc
     9541 taacgttaac aaccggtacc tctagataag taatgagagc tcgctgatca gcctcgactg
     9601 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9661 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9721 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9781 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9841 ccagctgggg ctcgagatcc actagttcta gcctcgaggc tagagcggcc gccactggcc
     9901 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9961 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    10021 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
    10081 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
    10141 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
    10201 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
    10261 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
    10321 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
    10381 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
    10441 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
    10501 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12421 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12481 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    12541 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    12601 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    12661 atgaggcgcg ccg
//
