LOCUS       sequence-408664-       12309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, 
            hPGK-DsRed) (pFM577).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Marayati BF, Thompson MG, Holley CL, Horner SM, Meyer KD
  TITLE     Programmable protein expression using a genetically encoded m(6)A 
            sensor.
  JOURNAL   Nat Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3.
  PUBMED    38168988
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 2. doi: 10.1038/s41587-023-01978-3."
COMMENT     Sequence Label: pGEMS-II
FEATURES             Location/Qualifiers
     source          1..12309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(311..329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(425..443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(460..1638)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          469..1407
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(492..512)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    join(1894..1912,1913..1935,1936..1954,1955..1977,1978..1996,
                     1997..2019,2020..2038,2039..2061,2062..2080,2081..2103,
                     2104..2122,2123..2145,2146..2164)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    2271..2388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2533..3009)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     regulatory      3804..3813
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3881..4084)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3910..3930)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4085..4464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4636..4655
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4730..4834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4835..4903,4904..5695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5053..5072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5866..6454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6355..6374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6608..6625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6742..6763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6778..6783,6784..6801,6802..6808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6816..6832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6821..6843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6840..6856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6840..6856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6905..7026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6916..6935)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6970..6989
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7156..7530)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7549..7596)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7644..7973)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7658..7793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7712..7731)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7958..7978
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7987..8415)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8097..8118)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8309..8328
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8461..8685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             8717..8897
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             complement(8921..9598)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(8983..9006)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(9181..9200)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9398..9418
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(9616..10126)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9679..9697)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    11104..11215
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(11257..11283)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(11257..11283)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(11920..12309,1..297))
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 ggccctacta cattcgccgc atgggctcca gctgagaaac caggtaatgc tgcaccttgt
       61 gttcggacag aaatatcttt ctgtcgtgaa cttctcgatg aagttgactt cgacgtgctt
      121 gttagtgttc tgtgatgtat gtcgccaaat ggagtgccgg cccccccaat taatttcgta
      181 aagcaggcag gtctccttgc ggagctctct cggatcgaag aatacctcaa actcatgggg
      241 ctcgatccgc cgtctcaatg tggggtccac agccactggg ccagtctctg agctcatggt
      301 ggcggctctc cctatagtga gtcgtattag cggccgccac tgtgctggat atctgcagaa
      361 ttccaccaca ctggactagt ggatccgagc tcggtaccaa gcttacctag ccagcttggg
      421 tctccctata gtgagtcgta ttagtaccaa gctaattcct cacgacacct gaaatggaag
      481 aaaaaaactt tgaaccactg tctgaggctt gagaatgaac caagatccaa actcaaaaag
      541 ggcaaattcc aaggagaatt acatcaagtg ccaagctggc ctaacttcag tctccaccca
      601 ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc caacctaaag acgacgtact
      661 ccaaaagctc gagaactaat cgaggtgcct ggacggcgcc cggtactccg tggagtcaca
      721 tgaagcgacg gctgaggacg gaaaggccct tttcctttgt gtgggtgact cacccgcccg
      781 ctctcccgag cgccgcgtcc tccattttga gctccctgca gcagggccgg gaagcggcca
      841 tctttccgct cacgcaactg gtgccgaccg ggccagcctt gccgcccagg gcggggcgat
      901 acacggcggc gcgaggccag gcaccagagc aggccggcca gcttgagact acccccgtcc
      961 gattctcggt ggccgcgctc gcaggccccg cctcgccgaa catgtgcgct gggacgcacg
     1021 ggccccgtcg ccgcccgcgg ccccaaaaac cgaaatacca gtgtgcagat cttggcccgc
     1081 atttacaaga ctatcttgcc agaaaaaaag cgtcgcagca ggtcatcaaa aattttaaat
     1141 ggctagagac ttatcgaaag cagcgagaca ggcgcgaagg tgccaccaga ttcgcacgcg
     1201 gcggccccag cgcccaggcc aggcctcaac tcaagcacga ggcgaagggg ctccttaagc
     1261 gcaaggcctc gaactctccc acccacttcc aacccgaagc tcgggatcaa gaatcacgta
     1321 ctgcagccag gtggaagtaa ttcaaggcac gcaagggcca taacccgtaa agaggccagg
     1381 cccgcgggaa ccacacacgg cacttacctg tgttctggcg gcaaacccgt tgcgaaaaag
     1441 aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg gaaaaaggcg
     1501 gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct aggcaccggt
     1561 tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc gctctgccca
     1621 ctgacgggca ccggagccaa ttcccactcc tttcaagacc tagaaggtcc attagctgca
     1681 aagattcctc tctgtttaaa actttatcca tctttgcaaa gctttttgca aaagcctagt
     1741 caataatcaa tgtcaacgcg gctcgccgtg aggagggggg cgcccgcggg aggcgccaaa
     1801 acccggcgcg gaggccagat ctggagccga cacgggttac cagtgtgctg gaattcgccc
     1861 ttcatgctag tggatccccc tcgagtttac cactccctat cagtgataga gaaaagtgaa
     1921 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     1981 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2041 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2101 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2161 gagaaaagtg aaagtcgggg ctgcaggaat tcggatccaa gggcgaattc tgcagatatc
     2221 catcacactg gtaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
     2281 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     2341 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     2401 actgccattt gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga
     2461 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt ccgagagaat
     2521 gcacaatgag gcttatcgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc
     2581 atcagcatcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
     2641 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg
     2701 caagaactgt tcataaacgc gaccgccgcc aataaccatg atttctggta cgtcaccaca
     2761 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tactcggttg
     2821 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaggtatg
     2881 gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
     2941 caggttccac ggcatgacgg tttccatgcc gataacgtga tctaccgcta acgccgcaat
     3001 cagactgata agccaagatg agattggcat gttttctgcg caagttaggt tttgtcgaga
     3061 aagggtgtaa cgcaactgtc gtaagtccgc cttgtacagc tcatccatgc cgagagtgat
     3121 cccggcggcg gtgacgaact ccagcaggac catgtgatcg cgcttctcat tgggatcttt
     3181 gctcagggcg gactgggtgc tcaggtagtg attatcgggc agcagcacgg ggccatcgcc
     3241 gatgggggta ttctgctggt agtgatcggc gagctgcacg ctgccatcct cgatattgtg
     3301 gcggatcttg aaattcacct tgatgccatt cttctgctta tcggccatga tatagacatt
     3361 gtggctattg taattgtact ccagcttgtg ccccaggata ttgccatcct ccttgaaatc
     3421 gatgcccttc agctcgatgc gattcaccag ggtatcgccc tcgaacttca cctcggcgcg
     3481 ggtacggtaa ttgccatcat ccttgaagaa gatggtgcgc tcctggacgt agccttcggg
     3541 catggcggac ttgaagaaat cgtgctgctt catgtgatcg gggtagcggc tgaagcactg
     3601 cacgccgtag gtgagggtgg tgacgagggt gggccagggc acgggcagct tgccggtggt
     3661 gcagatgaac ttcagggtga gcttgccgta ggtggcatcg ccctcgccct cgccggacac
     3721 gctgaacttg tggccattta catcgccatc cagctcgacc aggatgggca ccaccccggt
     3781 gaacagctcc tcgcccttgc tcaccatggc ggctctagag ggcccgttta aacgctagcg
     3841 cggccgcgga tctctagcgg atctgacggt tcactaaacc agctctgctt atatagacct
     3901 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     3961 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     4021 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     4081 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     4141 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     4201 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     4261 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     4321 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     4381 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     4441 aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg
     4501 caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct tgccttgttg
     4561 tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca gggagcagat
     4621 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aggggatcgg
     4681 gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4741 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4801 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4861 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4921 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4981 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5041 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5101 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5161 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5221 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5281 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5341 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5401 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5461 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5521 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5581 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5641 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5701 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5761 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6001 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6661 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6721 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6781 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6841 aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg gtatacagac
     6901 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6961 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     7021 caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     7081 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     7141 atctcgtagc acgtgtcagt cctgctcctc ggccacgaag tgcacgcagt tgccggccgg
     7201 gtcgcgcagg gcgaactccc gcccccacgg ctgctcgccg atctcggtca tggccggccc
     7261 ggaggcgtcc cggaagttcg tggacacgac ctccgaccac tcggcgtaca gctcgtccag
     7321 gccgcgcacc cacacccagg ccagggtgtt gtccggcacc acctggtcct ggaccgcgct
     7381 gatgaacagg gtcacgtcgt cccggaccac accggcgaag tcgtcctcca cgaagtcccg
     7441 ggagaacccg agccggtcgg tccagaactc gaccgctccg gcgacgtcgc gcgcggtgag
     7501 caccggaacg gcactggtca acttggccat ggtttagttc ctcaccttgt cgtattatac
     7561 tatgccgata tactatgccg atgattaatt gtcaacacgt gctgatcaga tccgaaaatg
     7621 gatatacaag ctcccgggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     7681 tacttctgga atagctcaga ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt
     7741 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     7801 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     7861 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     7921 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     7981 agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     8041 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     8101 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     8161 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     8221 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     8281 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     8341 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     8401 cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc gcctcagaag
     8461 ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     8521 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     8581 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     8641 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag gctgatcagc
     8701 gggtttaaac gggccctgct agagattttc cacactgact aaaagggtct gagggatctc
     8761 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     8821 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     8881 tctaaccaga gagacccagt acagtccgga ccagctagcc ctactggaac aggtggtggc
     8941 gggcctcggc gcgctcgtac tgctccacca cggtgtagtc ctcgttgtgg gaggtgatgt
     9001 ccagcttgga gtccacgtag tagtagccgg gcagcttcac gggcttcttg gccatgtaga
     9061 ttgacttgaa ctccaccagg tagtggccgc cgcccttcag cttcagcgcc ttgtggatct
     9121 cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggagggctcc cagcccagag
     9181 tcttcttctg cattacgggg ccgtcggagg ggaagttcac gccgatgaac ttcacgtggt
     9241 agatgaaggt gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct
     9301 cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc ttgtagtcgg
     9361 ggatgtcggc ggggtgcttc acgtacacct tggagccgta ctggaactgg ggggacagga
     9421 tgtcccaggc gaagggcagg gggccgccct tggtcacctg cagcttggcg gtctgggtgc
     9481 cctcgtaggg cttgccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc
     9541 cctccatgtg caccttgaag cgcatgaagg gcttgatgac gttctcagtg ctatccatgg
     9601 ttgtgctagc tacagctggg gagagaggtc ggtgattcgg tcaacgaggg agccgactgc
     9661 cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc gcgggcagga acagggccca
     9721 cactaccgcc ccacaccccg cctcccgcac cgccccttcc cggccgctgc tctcggcgcg
     9781 ccctgctgag cagccgctat tggccacagc ccatcgcggt cggcgcgctg ccattgctcc
     9841 ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg gcttccgttt gtcacgtccg
     9901 gcacgccgcg aaccgcaagg aaccttcccg acttaggggc ggagcaggaa gcgtcgccgg
     9961 ggggcccaca agggtagcgg cgaagatccg ggtgacgctg cgaacggacg tgaagaatgt
    10021 gcgagaccca gggtcggcgc cgctgcgttt cccggaacca cgcccagagc agccgcgtcc
    10081 ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccgtgg aattggatcc
    10141 gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg caggcattca
    10201 aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttggcgacc ctgcacatag
    10261 cagggggttg gagctgggtg agcactgtgc tcctttgcaa cccaggccat tctatgattc
    10321 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
    10381 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcgcgtgcca ccttccccgt
    10441 gccgggctgt ccccgcacgc tgccggctcc ggctcgggga tgcgggggga gcgccggacc
    10501 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg aagagggatg caattacatc
    10561 cctgggggct ttggcggggg gctctccccg tgagctcccg cggaaggtta attaactgac
    10621 agccacgatc ccataggtga aggcaaaggc gtggcagcta ggaacactca ctacaccccc
    10681 actcaaggga acaccgtgcc cagctaccct gagacagccc cactcccagg aaatgcaggt
    10741 gccaaccagc cccagtgagg catggcacct gagccagaca cccccaaata gtcccttccc
    10801 acctcctcaa gcatctgcac tctgggtgaa cccagaaaaa ctggggtacc tggggagaca
    10861 tagaagggcc aggccaggaa ctccccaata agcaggaaca gagacctgac ccctgagcct
    10921 cccccaccct ctaaggctgc tcaatgtcaa ggcaggaggg atctccacac acctgcactc
    10981 tgggtaaggt agaattcgtt aacctcgagc ccagcatgcc tgctattgtc ttcccaatcc
    11041 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
    11101 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
    11161 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
    11221 gctgatcagc gggtttaagt ttcgggttta aactcaggcg tagtcgggca cgtcgtaggg
    11281 gtactcgagt ttcccacgac cttgacgttc ctttttaaca ctttcttctt cctcttggcg
    11341 tttctcatag tgtgagaagt catcaaaaat ggaagtggtg tgcttgtagc tggctataat
    11401 tttcaacacc tgcttagcct tttccagagg cacttcctga gtgtccctag agttggtcac
    11461 tggtttattc tcgttgttct ctaggcgaat gtgtcgcagt tggctattgg gaacgtcctt
    11521 cacaaaaatc cacctgacat caaaacgacc cttccatttg tcctgggacc acacacctgc
    11581 acatgtgttg tagtccacag cagatttcat ttctgccacg ccacagaagt gtccactgcc
    11641 gttgacactg aaaagtaagt aaacggggcc tttcccgttc atggaacgat aagcagcatc
    11701 cagtctcttg ttaccatgct ctgtgctgca ccaaatatta tacttaatgg aacggtgaat
    11761 atcgtcctca gagtagctct taatgatgaa aacccggcca tgtttcagat tccagtcaaa
    11821 atctttgggg ttatagttat taatggaccg aagcttctcc aacactgggt ggggttctgg
    11881 tgtggcggac tctgaggtcc cgggagtctc gctgccgctt ttcaacccgg tggcccagag
    11941 aatgtgtggg ggcagtcgct ggtaatgaca agactgaaga gcgatggtaa agaatgtcag
    12001 ctgtggctgc ttccttctca gaatgttgag acaaggaggc aggcccagta tgatgcagta
    12061 cagttcaaga acgtacagtc gtacccacag atggggatac ctaggccagt gggcttcatt
    12121 actcgggcta taattcacaa agtttctcca gcagtatcct gactcctgct cagtcataat
    12181 ttggatagtc acacctgaag agatcaaatc ccgcaggcct tgtcgattgc gggggtcagc
    12241 gtggtggtac agccttgcga tgtaaataaa cagagtgacg tggggatacc ttgacaggaa
    12301 ttcagtgat
//
