LOCUS       sequence-408686-       10621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of Need1 fused to mOrange2 and rat (r) 
            Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to 
            activate gamma-TuRC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: 
            pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1
FEATURES             Location/Qualifiers
     source          1..10621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3283..3303)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3306..3315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    4424..4457
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6549..7256
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7540..7557)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7546..7770
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7842..7859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7842..7858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7851..7873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7999..8454)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8136..8157)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8348..8367
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8481..8585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8586..8654,8655..9446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8804..8823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9617..10205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10106..10125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10359..10376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10493..10514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10529..10534,10535..10552,10553..10559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10567..10583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10572..10594
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10591..10607
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10591..10607
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgrkgggg gsgggggggg ggggggggsg sgsgssrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggsggr grggkgsggs ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggkggsgg crggkggggg kgcsgggsgg
     1261 ggcggggccg ccksgggssg gggrgggsks gggggrgggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tcttcagtcc ccatcctcga gcagcctcac
     2281 catggcccca gtctcagagt ccaggtttct gatgtagttc atcacaatgt tcacattggt
     2341 ccagccacca gcctgcatga tttcagggat ggacacacca gccctggcca tgtccctggc
     2401 agcacccact ctggcagagt ggccagacca ggccaggtat ctctgcccag agtcatcctt
     2461 ggcaccatag atcaggcggt gggtggcctc aaagatccct tccagggccc gggtggacag
     2521 ttgggaggtg gcagaagggg cagccacacc attctttctg acccggcaga acaggtagtt
     2581 gttggggtca tcagccacac cagacacaga gatccatctc tccaccagct tggtaacccc
     2641 cagggacagg gccttctcca caccagctgt ggacaccagg gtcttggtcc tgccaatgtg
     2701 gatcagcatt ctcccaccat cggtgcggga gatgtccttc actctgattc tggcaatttc
     2761 ggcaatgcgc agcagggtgt tgtaggcaat gcccaggaag gccaggttcc tgatgtcctg
     2821 gcatctgtca gagttctcca tcagggatct gacttggtca aagtcagtgc gttcaaaggc
     2881 cagggcctgc ttggctctct ccccagcatc cacattctcc tttctgattc tcctcatcac
     2941 cagggacaca gcattggagt cagaagggcg aggcaggcca gatctcctgt gcagcatgtt
     3001 gagctggccc aggtgctgtt ggatggtctt cacagccagg cctctggctt gcaggtacag
     3061 gaggtagtcc ctcacatcct caggttcagc agggaaccat ttcctgttgt tcagcttgca
     3121 ccaggcagcc caggatctgc acacagacag gagcatcttc caggtgtgtt cagagaaggc
     3181 ctgcctgtcc ctgaacatgt ccatcaggtt cttcctgact tcatcagagg tggcatccac
     3241 agggagggca ggcaggtttt ggtgcacagt cagcaggttg gagactttcc tcttcttctt
     3301 gggcaccatg gtggcgaatt cgttgctgct tcgcggctgc cgggtggatg gcggaggcga
     3361 ggaggaggtg ttgcttctta cagcgcgact cttaagcggt cgatgtaaga gaacctgcgg
     3421 cacttgggcg gatgcgcctc cttatataca gctcggtcac catggggatg ggccaaggcc
     3481 ttttcctgtt ggtccagacc cctccgtctg cccagagaag ccgcgacagg agagtggtga
     3541 ttggtgcaag cgacatggta tgaggtaatc tctcccccct tccccccctc gctagcatcc
     3601 gtagattttg catccttcag acaaaggcct cgcaaaagca actagccgag catccattat
     3661 ggggtggagc tggggagggg tagagaggga agaaagaaaa cgggggttgg gtgattatac
     3721 ggagcttaaa ccttaaactg tagttaggat aatttaaggt tttccccaac acttaatcac
     3781 agaatttgtg ttgagattct ttatggtaaa ttccagcttt tcatttatac tacactaaat
     3841 ttaaatacgt tccgtcagga ttcaacatct ctgttcgctt taaataaagt agcaagagct
     3901 atgggaatga tggtcaaaaa ggcgtgggta aatgtttagt tttacagttg tattattgtg
     3961 gttattatta ttactattat tattaatatt tgagcaaaat tcagaagctc agtatgaata
     4021 gaccacgcct atgaccctca acagcacccg ggaggcagag gcaggaagac ttgcagctca
     4081 aggtcatcat aaccgccctt agtggtttat gcctgtaaat cccagcattg ggaagcggag
     4141 gactgcggtt attttgtacc cggcctggat atatagtgtg atatcctgtc tcaaaaacca
     4201 aaaacaaaca aagagagaga gagagagaaa ggaaaaaaga gaaacaatat acaaagtagt
     4261 acttaacgct ttactagaga gcttgctttc tttagcggta gtgctctccc atcacccctc
     4321 cccgctcgcc cccttacccg accctacctc accccagagc ctgtgttatt ttgtccctag
     4381 aaatgagcca tccctcctaa tacggaattc cggaaccctt aatataactt cgtataatgt
     4441 atgctatacg aagttattag gtccctcgac ctgcagcctc cgcagccccc aagctagcag
     4501 ccaccatgca ggaaaacctc agatttgcct catcaggaga tgatgttaaa atatgggatg
     4561 cttcgtttct gacattggtg gataaattca acccccacac gtcaccgcat ggaatcagtt
     4621 caatctgttg gagcagcaat aataacttcc tagtgactgc gtcttctggt ggtgacaaaa
     4681 tagtggtgtc aagttgcaaa tgtaagcccg ttccactttt ggagcttgca gaagggcaaa
     4741 agcagacatg tgtcgattta aattctacat caatgtattt ggcaagtgga ggcctaaata
     4801 acactgttaa tatttgggat ttaaagtcaa aacgacttca tcggtctctt aaggaccata
     4861 aacgggaagt aacttgtgtg gcgtacaatt ggaatgattg ttacattgct tccgggtctc
     4921 tcagtggtga gattatctta cacagcgtaa ccactaatac ctccagtacg cctttcggcc
     4981 atgggagtaa gcagcctgtc cggcacatca agtactcgtt gtttaggaag tctctcttgg
     5041 gcagcgtttc ggataatgga gtagtgaccc tctgggatgt gaacagtcag agtgcatacc
     5101 acagctttga cagtacacac aaagccccag cttcaggcct ctgcttctct cctgtcaacg
     5161 aactgctgtt tgtgaccata ggcctggata aaagaatcat cctgtatgac acttcaagta
     5221 agaagctggt gaaaacttta gtggctgaca ctcctctgac tgctgtagat tttatgccag
     5281 atggagccag tttggctatt ggatcctccc gtgggaaaat atatcagtat gacttaagga
     5341 tgctgaaatc tcccgtgaaa accatcagcg cccacaagac atctgtgcag tgtatagcat
     5401 ttcagtactc cacatctctg acgaaggcaa gtttaaataa aggctgctca aataaggcca
     5461 cggcagcgaa taaacggagt gttcctgtgg gcagcagcag tggggctgcc cagaattctg
     5521 gaattgtcag agaagcaccc agcccttcca ttgccacagt tctcccacag cccgtgacga
     5581 cagctgtggg caaaggagca ggggctgctc aggacgaagc aggtttgcct cgaagcaaaa
     5641 atacagatat cttatctaag gaaactgatg ctggaaaaag tcaagacttc tccagctttg
     5701 atgacacggg gaagagtagt ttaggggaca tgttttcacc aattagagat gatgctgtag
     5761 tcaacaaagg aggtgacgag tctgtaggca aaggagatgg ccttgacttt ctaccacagc
     5821 taaactcggt gttccccctg aggaagaatg caggctcctc cagcactctg gtgctgcact
     5881 ccagcccttt taatgtcctt atggggacag ggaaggaaga gcatgagagt cacgagctgt
     5941 cagccgagtc taagaaggca caccttggga agccggagcc taaggactcc ttgaagcagt
     6001 ttacaaagtt gatctcctct ggtgctgaac ctggaattct aaacacctgt ccatcatcta
     6061 accaagcaag aaatcttgaa aagtttgaaa agccagaaaa agatattgaa gtccagttga
     6121 tgcacgaacc ctcagtcaat ggaccctcaa ccccagtacc aaaggcagcc tcctccgtca
     6181 ctgctggagt agccagttca ctctcagaaa aaatagtcga cacccttggg aacagtcggc
     6241 cggccgcgcc acttacctct gtccagatcc gcttcattca gaacatgata caagaaaccc
     6301 tggatgactt cagggaagct tgccataggg acattgtgaa tctgcaagtg gaaatgatta
     6361 aacagtttca tatgcaactg aatgaaatgc actctttgct ggaaagatac tcagtgaatg
     6421 aaggcttagt ggctgagatc gaaaggctgc gagaagaaaa caaaaggctg cgggcccact
     6481 tctcaagcct cctcgacggc agcaagcttg gtggaggcag cgcatcaggc ggaagcggaa
     6541 gcagatctat ggtgagcaag ggcgaggaga ataacatggc catcatcaag gagttcatgc
     6601 gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     6661 gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc aagggtggcc
     6721 ccctgccctt cgcctgggac atcctgtccc ctcatttcac ctacggctcc aaggcctacg
     6781 tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag ggcttcaagt
     6841 gggagcgcgt gatgaactac gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     6901 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     6961 gccccgtgat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     7021 aggacggtgc cctgaagggc aagatcaaga tgaggctgaa gctgaaggac ggcggccact
     7081 acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     7141 acatcgtcga catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     7201 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtctg
     7261 gcacaggtgg aagcatcgat aaggtctctc ccaccagagc ccggaacatg aaggactttg
     7321 aaaaccaaat cactgagctg aagaaggaga acttcaatct aaagctccgg atctacttcc
     7381 ttgaggagag gatacagcag gagtttgctg gccccacaga gcacatctac taagcggccg
     7441 ccatatgcat cctaggccta ttaatattcc ggagtatacg tagccggcta acgttaacaa
     7501 ccggtacctc tagataagta atgagagctc gctgatcagc ctcgactgtg ccttctagtt
     7561 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     7621 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7681 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7741 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7801 cgagatccac tagttctagc ctcgaggcta gagcggccgc cactggccgt cgttttacaa
     7861 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     7921 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     7981 agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     8041 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     8101 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     8161 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     8221 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     8281 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     8341 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     8401 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     8461 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8521 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8581 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8641 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8701 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8761 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8821 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8881 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8941 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9001 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9061 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9121 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9181 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9241 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9301 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9361 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9421 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9481 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9541 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9661 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9721 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9901 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9961 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10021 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10081 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10141 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10201 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10261 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10321 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10381 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10441 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10501 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10561 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gaggcgcgcc
    10621 g
//
