LOCUS       sequence-408700-        5761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc expressed constitutively from PBT1311, pNBU2 backbone, AmpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Mimee M, Tucker AC, Voigt CA, Lu TK
  TITLE     Programming a Human Commensal Bacterium, Bacteroides 
            thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut 
            Microbiota.
  JOURNAL   Cell Syst. 2015 Jul 29;1(1):62-71. doi: 10.1016/j.cels.2015.06.001.
  PUBMED    26918244
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2015.06"; journalName: "Cell Syst"; date: 
            "2015-07-29- 29"; volume: "1"; issue: "1"; pages: "62-71"
COMMENT     Sequence Label: pMM553
FEATURES             Location/Qualifiers
     source          1..5761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3761..3829,3830..4621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3979..3998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            4752..4861
                     /note="incP origin of transfer"
     rep_origin      5032..5420
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 atggttttta ctctggaaga ttttgttggc gattggcgtc agaccgcggg ttataatttg
       61 gatcaagtcc tggaacaggg tggcgtaagc tctctgttcc agaacctggg tgtgagcgtg
      121 acgccgattc agcgcatcgt tctgtccggc gagaacggtc tgaaaattga tattcatgtg
      181 atcatcccgt acgaaggcct gagcggtgac caaatgggtc aaatcgagaa aatctttaaa
      241 gtcgtctacc cagttgacga tcaccacttc aaggttatct tgcattacgg tacgctggtg
      301 attgatggtg tgaccccgaa tatgattgac tatttcggcc gtccgtatga aggcattgcc
      361 gtttttgacg gtaaaaagat caccgtcacc ggtaccctgt ggaatggcaa taagattatt
      421 gacgagcgtc tgattaaccc ggacggcagc ctgctgttcc gcgtgaccat caacggtgtc
      481 acgggttggc gtctgtgcga gcgcatcctg gcataatgaa ctgcacttgc tttgataatt
      541 aatgataaac aatctaaaag cactctaatc gttatcggag tgcttttaga ttactaatca
      601 aattgcttct actaattgcc tatcttccag tgatggaaca gcatttgtgc attggctgca
      661 acaatcagcc ttacttgtgc ctgttctatt tccgaaccga ccgcttgtat gaatgcatca
      721 aaattcgttt tctctacgtt ggattccttg ttgctcatat tgtgatgata atttctacaa
      781 atatagtcat tggtaactat ctatgaaact gtttgatact tttatagttg attaaacttg
      841 ttcatggcat ttgccttaat atcatccgct atgtcaatgt agggtttcat agctttgtag
      901 tcgctgtgtc ccgtccattt catgaccacc tgtgccggga ttccgagagc cagcgcattg
      961 cagatgaatg tcctttttcc tgcatgggta ctgagcaaag cgtatttggg tgtgacttca
     1021 tcaatacgtt catttccctt gtagtaggtt tcccgtacag gctcgttgat ttctgccagt
     1081 tcgcccagct ctttcaggta atcgttcatc ttctggttgc tgatgacggg cagagccatg
     1141 taattctcga aatggatgtc cttgtatttg tccagtatgg ctttgctgta tttgttcagt
     1201 tcaatcgtca ggctgtcggc agtcttgact gtggttattt cgatgtggtc ggacttcaca
     1261 tcgcttcttt tcagattgcg aacatccgaa taccgcaaac tcgtaaagca gcagaacagg
     1321 aaaacatcac gcacacgttc caggtattgc ttatccttgg gtatctggta gtctttcagc
     1381 ttgttcagtt catcccaagt caggaagatt acttttttcg aggtggtttt cagtttcggt
     1441 ttgaacgtat cgtatgcaat gttctgatga tgtcctttct tgaagctcca gcgcaggaac
     1501 catttgagga atcccatttg cttgccgatg gtgctgtttc tcatatcctt ggtgtcacgc
     1561 aggaagttga cgtattcgtt caatccaaac tcgttgaaat agttgaacgt tgcatcctcc
     1621 ttgaactctt tgaggtggtt cctcactgct gcaaattttt cataggtgga tgccgtccag
     1681 ttattctggt taccgcactc ttttacaaac tcatcgaaca cctcccaaaa gctgacaggg
     1741 gcttcttccg gctgttcttc actggtgtct ttcattctca tgttgaaagc ttccttcaac
     1801 tgttgggtcg ttggcatgac ctcctgcacc tcaaattcct tgaaaatatt ctggatttcg
     1861 gcatagtatt tcagcaagtc cgtattgatt tcggctgcac tttgctttag cttgttggta
     1921 catccgttct ttacccgctg cttatctgca tcccatttgg ctacgtcaat ccggtagccc
     1981 gttgtaaact cgatacgttg gctggcaaag atgacacgca tacggatggg tacgttctct
     2041 acgattggca caccgttctt tttccggctc tccaatgcaa aaatgatgtt gcgcttgata
     2101 ttcataattg ggtgcgtttg aaattctaca cccaaatata cacccaatta ttgagatagc
     2161 aaaagacatt tagaaacatt tacttttact ctatattgta atttacactt gattatcagt
     2221 cgtttgcagt tttatgatat tctgtgaaag tataagttcg agagcctgtc tctccgcaaa
     2281 aaacgctgaa aatcagcaga ttgcaaaaca aacaccctgt tttacaccca agaatgtaaa
     2341 gtcgggtgtt tttgttttat ttaagataat acaaccacta cataataaaa gagtagcgat
     2401 attaaaagaa tccgatgaga aaagactaat atttatctat ccattcagtt tgatttctca
     2461 ggactttaca tcgtcctgaa agtatttgtt ggtaccgagg acgcgtaaac atttacagtt
     2521 gcatgtggcc tattgttttt agccgttaaa tattttataa ctattaaata gcgatacaaa
     2581 ttgttcgaaa ctaatattgt ttatatcata tattctcgca tgttttaaag ctttattaaa
     2641 ttgatttttt gtaaacagtt tttcgtactc tttgttaacc catttcatta caaaagtttc
     2701 atattttttt ctctctttaa atgccatttt tgctggcttt ctttttaata caattaatgt
     2761 gctatccact ttaggttttg gatggaaata atacctagga atttttgcta atatagaaat
     2821 atctacctct gccattaaca gcaatgctag tgatctgttt gtatctaata acattttagc
     2881 aaaaccatat tccactatta aataacttat tgtggctgaa ctttcaaaaa caatttttcg
     2941 aattatattt gtgcttatgt tgtaaggtat gctgccaaat attttatatg gattgtggct
     3001 aggaaatgta aatttcagta tatcatcatt tactatttga tagttaggat aatttaagag
     3061 cttattacga gttacctcac ataatttaga atcaatttct atcgccgtta caaaattaca
     3121 tctctttacc aatccagcag taaaatgacc tttccctgca cctatttcaa agatgttatc
     3181 tttttcatct aaacttatgc aattcattat tttttctatg tgatattttg aagtaataaa
     3241 attttgacta tcttttatat ttactttgtt cattataacc tctccttaat ttattgcatc
     3301 tcttttcgaa tatttatgtt ttttgagaaa agaacgtact catggttcat cccgatatgc
     3361 gtatcggtct gtatatcagc aactttctat gtgtttcaac tacaatagtc atctattctc
     3421 atctttctga gtccaccccc tgcaaagccc ctctttacga cataaaaatt cggtcggaaa
     3481 aggtatgcaa aagatgtttc tctctttaag agaaactctt cgggatgcaa aaatatgaaa
     3541 ataactccaa ttcaccaaat tatatagcga cttttttaca aaatgctaaa atttgttgat
     3601 ttccgtcaag caattgttga gcaaaaatgt cttttacgat aaaatgatac ctcaatatca
     3661 actgtttagc aaaacgatat ttctcttaaa gagagaaaca cctttttgtt caccaatccc
     3721 cgacttttaa tcccgcggcc atgattgaaa aaggaagagt atgagtattc aacatttccg
     3781 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3841 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3901 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3961 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4021 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4081 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4141 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4201 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4261 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4321 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4381 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4441 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4501 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4561 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4621 actgtcagac caagtttact cataacgcgt caattcgagg gggatcaatt ccgtgatagg
     4681 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     4741 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     4801 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     4861 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     4921 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     4981 ttatgcagcg gaaaacggaa ttgatccggc cacgatgcgt ccggcgtaga ggatctgaag
     5041 atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg tactaagctc
     5101 tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg ctaacgtact
     5161 aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc tcatgtttga
     5221 acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa gttttataag
     5281 aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt ggacaacaag
     5341 ccagggatgt aacgcactga gaagccctta gagcctctca aagcaatttt gagtgacaca
     5401 ggaacactta acggctgaca tgggaattcc cctccaccgc ggtggcggcc gctgatctgg
     5461 aagaagcaat gaaagctgct gttaagtctc cgaatcaggt attgttcctg acaggtgtat
     5521 tcccatccgg taaacgcgga tactttgcag ttgatctgac tcaggaataa attataaatt
     5581 aaggtaagaa gattgtagga taagctaatg aaatagaaaa aggatgccgt cacacaactt
     5641 gtcggcattc ttttttgttt tattagttga aaatatagtg aaaaagttgc ctaaatatgt
     5701 atgttaacaa attatttgtc gtaactttgc actccaaatc tgtttttaac atatggcact
     5761 a
//
