LOCUS       sequence-408717-        2443 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of y-ATOM biosensor for HRas.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Sekhon H, Ha JH, Presti MF, Procopio SB, Jarvis AR, Mirsky PO, John 
            AM, Loh SN
  TITLE     Adaptable, turn-on maturation (ATOM) fluorescent biosensors for 
            multiplexed detection in cells.
  JOURNAL   Nat Methods. 2023 Nov 9. doi: 10.1038/s41592-023-02065-w.
  PUBMED    37945909
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 9. doi: 10.1038/s41592-023-02065-w."
COMMENT     Sequence Label: pCMV-MBP-yATOM_Ras
FEATURES             Location/Qualifiers
     source          1..2443
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..1182
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1156..1179
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1195..1218
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     1326..1347
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1453..1474)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct
       61 caagcttcga attctgccac catgaaaact gaagaaggta aactggtaat ctggattaac
      121 ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa agataccgga
      181 attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca ggttgcggca
      241 actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg ctacgctcaa
      301 tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct gtatccgttt
      361 acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc tgttgaagcg
      421 ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg ggaagagatc
      481 ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa
      541 gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt caagtatgaa
      601 aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa agcgggtctg
      661 accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga ttactccatc
      721 gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg
      781 tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac cttcaagggt
      841 caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac
      901 aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg tctggaagcg
      961 gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga agagttggcg
     1021 aaagatccac gtattgccgc cactatggaa aacgcccaga aaggtgaaat catgccgaac
     1081 atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc
     1141 ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag ctcgctggaa
     1201 gttctgttcc aggggcccaa ggtaccagga gctgcgacac cggtaactgc agacatgctg
     1261 cccgacaacc actacctgag ctatcagtcc gttctgagca aagaccccaa cgagaagcgc
     1321 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1381 ctgtacaagg gcggtggatc cggtggtatg gtgagcaagg gcgaggagct gttcaccggg
     1441 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
     1501 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagctgat ctgcaccacc
     1561 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctggcctgc
     1621 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1681 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
     1741 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1801 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc
     1861 tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
     1921 gttgaggacg gtgtggacta cacgattacc gtgtatgcgt ggggttggca tggccaggtt
     1981 tattactata tgggtagccc aatctcgatt aactaccgta ccggaggttc aggcagtggt
     2041 ggtgctagcg gtggtgcgac tggaggcagt ggcggtggat cttctgttcc gaccaaactg
     2101 gaggtggttg ccgcaactcc cacgagtctg ctgatctcat gggatgctcc agccgttacc
     2161 gtcgattatt acgtcatcac ctatggcgaa acaggcggca actctccggt acagaagttt
     2221 gaagtgcctg ggtccaaaag cactgcgacc atttcgggct taaaaccggg cagcgtgcag
     2281 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gtaaagcggc
     2341 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     2401 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgc
//
