LOCUS       sequence-408740-       12720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression of Akna fused to mOrange2. Used in 
            combination with Talpha1-iCre-pA plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, 
            Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, 
            Bradke F
  TITLE     Centrosomal microtubule nucleation regulates radial migration of 
            projection neurons independently of polarization in the developing 
            brain.
  JOURNAL   Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 
            10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
  PUBMED    36796357
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2023.01.020"; journalName: "Neuron"; date: 
            "2023-04-19- 19"; volume: "111"; issue: "8"; pages: "1241-1263.e16"
COMMENT     Sequence Label: pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2
FEATURES             Location/Qualifiers
     source          1..12720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          665..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1694..1713
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1694..1712
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1741..1774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2112..2131
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    2130..2185
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     2231..2250
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2260..3282)
                     /product="site-specific recombinase"
                     /label=Dre recombinase
                     /note="Dre is a site-specific recombinase from 
                     bacteriophage D6. Recombination occurs at rox sequences."
     CDS             complement(3289..3315)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3316..3336)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4471..4504
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      4547..4556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6836..6847
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     CDS             8822..9532
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9639..9656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9645..9869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9941..9958)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9941..9957)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9950..9972)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10098..10553)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10235..10256)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10447..10466
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10580..10684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10685..10753,10754..11545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10903..10922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11716..12304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12205..12224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12458..12475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12592..12613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12628..12633,12634..12651,12652..12658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12666..12682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12671..12693
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12690..12706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12690..12706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgrtgggg gcgggggggg ggggggggsg sgssssrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgrggsggr grggkgsggc ggcrgccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggkggcgg crggkggggg kgccgggcgg
     1261 ggcggggccg ccksgggssg gggrgggsks gggggrgggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccaa tcatttaaat
     1741 ataacttcgt ataatgtatg ctatacgaag ttattcgcga tgaataaatg aaagctgtcg
     1801 agggatcttc ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa
     1861 aatctggcta gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca
     1921 aaacaaaata taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg
     1981 acagcagggg gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca
     2041 gccagcatat gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
     2101 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
     2161 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
     2221 tccccataat ttttggcaga gggaaaaaga tctgactcag ctatccatca gtcgagaatt
     2281 ggctcccacg gacactccgc cccttctgag ataccgcatc agtgtttcag gcttcttcca
     2341 tgtcccttcc tgcataatct cgaccataga cacctgtttc tcggccatgt ctattgcggc
     2401 acccaccctg gcggaatggc cggtccaggt cctatacctc cctttattgg gggtggcgtc
     2461 ccgtttgttc agcagcaccc aggcatcact gaaaatcttc tccatggcag gggcggtcag
     2521 gggcgtggta gtaatgcggg ctttgtttga ccggtgtata ggtgggaaga gcacagcgtc
     2581 tggatgttct ctaagcccac tgacgtccag ccagtcattg agcactgctg tggttcgccg
     2641 ggagagcact ttgtcaaggc ccgcagctgt ggtgattgtc ttagtgtgac tgatgtgcag
     2701 ggtcactgtg tctcctgtct gatccaagtc cccaaccctg atgcgtgata tttcagacat
     2761 tctcatgagg gtgttatagg ctacgaacag gaaagccctg ttgcgcaggt cgaccagccg
     2821 ctcgctcctg ctgagcagca catccagcag cttcaggtcg tcccagcgca gggggattgc
     2881 ctgtcctgtc ctctcaccct tttcggttgc ggcttctctt ctaattcttc tcatggcaag
     2941 actcactgac ttgtcgtcgg acaagggtgg cagtccgcag tgggacagaa gcatattaag
     3001 catagcatag tgcttgtcga tggtagtgga ggccaggtct gcatcgtgca actgaagaaa
     3061 gtattcgcgt gccatttcag gactaattgg aaaccatgca agctgccgag catggcacca
     3121 tctggcccag ctatgaaaga ccaacctcaa atccctcaaa gtgttaggag cgtacgcccc
     3181 ctggtcattc ataaacctca tgaagttttc agcggcctcc tgatactctt tgccgatgtt
     3241 tcgcaggaat ccaccagatg aaccactaat aatcagctca gaggatccag cgtaatctgg
     3301 aacatcgtat gggtaaacct tcctcttctt cttaggcatg gtggcgaatt cgttgctgct
     3361 tcgcggctgc cgggtggatg gcggaggcga ggaggaggtg ttgcttctta cagcgcgact
     3421 cttaagcggt cgatgtaaga gaacctgcgg cacttgggcg gatgcgcctc cttatataca
     3481 gctcggtcac catggggatg ggccaaggcc ttttcctgtt ggtccagacc cctccgtctg
     3541 cccagagaag ccgcgacagg agagtggtga ttggtgcaag cgacatggta tgaggtaatc
     3601 tctcccccct tccccccctc gctagcatcc gtagattttg catccttcag acaaaggcct
     3661 cgcaaaagca actagccgag catccattat ggggtggagc tggggagggg tagagaggga
     3721 agaaagaaaa cgggggttgg gtgattatac ggagcttaaa ccttaaactg tagttaggat
     3781 aatttaaggt tttccccaac acttaatcac agaatttgtg ttgagattct ttatggtaaa
     3841 ttccagcttt tcatttatac tacactaaat ttaaatacgt tccgtcagga ttcaacatct
     3901 ctgttcgctt taaataaagt agcaagagct atgggaatga tggtcaaaaa ggcgtgggta
     3961 aatgtttagt tttacagttg tattattgtg gttattatta ttactattat tattaatatt
     4021 tgagcaaaat tcagaagctc agtatgaata gaccacgcct atgaccctca acagcacccg
     4081 ggaggcagag gcaggaagac ttgcagctca aggtcatcat aaccgccctt agtggtttat
     4141 gcctgtaaat cccagcattg ggaagcggag gactgcggtt attttgtacc cggcctggat
     4201 atatagtgtg atatcctgtc tcaaaaacca aaaacaaaca aagagagaga gagagagaaa
     4261 ggaaaaaaga gaaacaatat acaaagtagt acttaacgct ttactagaga gcttgctttc
     4321 tttagcggta gtgctctccc atcacccctc cccgctcgcc cccttacccg accctacctc
     4381 accccagagc ctgtgttatt ttgtccctag aaatgagcca tccctcctaa tacggaatga
     4441 tccccatcaa gctgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     4501 ttattaggtc cctcgacctg cagcctccgc agcccccaag ctagcagcca ccatggccag
     4561 ctcaggggca aaggcacaat gggttgggcc aagcctgggg caagggcccc gaaggcggcg
     4621 ctgggcttgg gctgaggaac aagatacaga tggaagaaga gaccagggct ggggaaacag
     4681 ccagtctctt cccgaagccc ccagtcctga gctcctagag gacttccgtc gggcacagga
     4741 gcacctgcca cccctggagt gggacccaga catgcaggac tccgaagagt cttctggaga
     4801 agagaccgaa gctgacgatg caagcagccc agagggttcc accgtgcctc tgccctggct
     4861 ctccaggagc aaccaacagc tggacatgtc tgaagaggag ctggatgagg cctctgggag
     4921 gcctgaggta gacttagctg gagagagttg tacggagctt gagtgtgaag accagggaga
     4981 ttccagccct ccacctccag gacaggggcc agccaaaggc tgggtcacct ttatcaaaca
     5041 aggcagtaac tacagacctt cagagcatct cgaggcccag ccatctgttg aacatagccg
     5101 cacaaagtct tggagcagtg gaactgtgag cctccgtcaa cctagtgaca gccttggttc
     5161 tacctgggaa ggagataccg aagtccctca gccttccatc ttgcccaaag ccctgccaca
     5221 gagtccgtgc cacaactttc cacacccagg tgacagaaat ggaggtgacg ttgctccggc
     5281 aacacccaca gaattccggg actccttagc agcgccagcc cagaatgccg agtgctctgc
     5341 aggcacgtgg ggggaagaaa ccaccagcct acccagctct cggcctgagg accaaacctg
     5401 gaagaggact aagacatcac ctaagccact gccctcccgg tttactggtt ctgtcagccc
     5461 cctgagtact cggcttgggg caataaagaa agttgtgccc cagcacaagc agggagccac
     5521 tctggctggt cactcttctt cacaagctcc caagtatggc agggggcgcc gcctgaacta
     5581 cccactcccc gacttctcca aggtggggcc ccgggtgagg tttcccaaag atgagaacta
     5641 ccgacccccg aagtccagag gccacaacag gcaacaaggc tctaccagac ccctcatctt
     5701 caagtcgcca gctgagattg ttcgggatgt actgttgagt agtggagagg cgtccctggc
     5761 caaggaatct tctcttgctc acaccatcac cagggtgccc caggagttcc agacacccga
     5821 acaagccact gagctagttc atcagctcca ggaggactat cacaagctcc tcaccaagta
     5881 tgcggaggct gagaacacta tcgaccaact tcgccttggg gccaaggtgc acctgtattc
     5941 agacccacca cagcccagtc agagcttctg ctcggggtca atgccacaag gaagcaaggt
     6001 cttatccttc tctatcccgc aacccagagt agcagagtgg tggcctgacc ctgcccagga
     6061 tccacaggcc tctgaggcca cagggtggcc gttcccacga acagacctga gtccctcctc
     6121 gtcccccggc gtggccactc cagggcggct tcctcagagc cagggcattg ccacagacca
     6181 gccctccaca ggacagaccc aggcactgac ctctcaggcc agccggctcc tggccaaggt
     6241 gcagtccttc gaggaactgg tactggctgg acacctccca cctcaggacc aaatcaagag
     6301 cttggagcaa ctgagggcag cccacatggc cctagaggca gagtacctgc aggcctgcag
     6361 agaagagctc ctggaccctc agcttgatgc ctctcagggg agtcccagga ccctcaacct
     6421 ctgcagggag ctagaagcag agatatacca cctgggacag cgcctggaag agctacagga
     6481 ccatatggac cagacccaaa gagagacaga gccatgtagg ccagacctgc aagacagcac
     6541 cccaaccatg tcctttctgc cccagtcagc tcatctgtcc atgccttcag gacctgtctc
     6601 cttgccagat ggccaaacct accaggagcc tgccaccacc accaccacct cccctggaag
     6661 ctcttgcacg ctccccatca acaagaagtt aagcctgagc atcaaaactg aggaaagccc
     6721 aagaggtctc ccggtcccac tcagggacag gacgcttcag gtggagcagg acttccatgg
     6781 ccttctggag agatacctca gcgttaaatc cctccctgaa gccttgaggg atgaagatga
     6841 ggatgacctg gaggaggagg aagaggagca ggaccatcaa ggccccttgg aagttgatag
     6901 tccagccaca gctccaggga aaacagaagc cgtgagggta ccaccaggag agcgcccaac
     6961 acaggctgag gaaagccata gggatgctac ccaggaggat gaggaacaga tgggacctat
     7021 gaagtcacca gacttccggc catccatggc cagagatacg tacacaccag tcctggatac
     7081 agctgaagtg gctcaacggg gcaccaaagc catggtgtca catcagagca gtctgaccag
     7141 cctggaagag agcaggccct cagagctcct tcctcgaaaa gccctgcttc gggctggtgg
     7201 gccccacact gaggagccct ggatggtgtc accagagaca gacagtggct tcgtgggctc
     7261 agaaaccagt atagtgtcac ccttcaccca gaccccagag caccggctct cccatgtcag
     7321 cacttcagga ccatcagctc aacacctcac tgcctctgtg cctggtgata gaacctccca
     7381 tcccaaggcc aggggtctca tggttcccag aagagccact gagactggca tacccagaag
     7441 ccgaacccag cagcatttct ccagcctgag ctctcccggg agaggggcac agagctgcca
     7501 cctagaggag acatcagtgg ctaaaatagc ggttcccagg tctgagttca agagacagaa
     7561 gcagatttcc aaacagctcc ttcccagtgg aagaaccagc cccgactctg ccccagctcc
     7621 cacagctgcc tccacgcccc atggatcagc agagagcact gccaacctcc tcctcaacag
     7681 gacagagcgc gaccaggcca tcaaggatct gcaggcggag gtgtcacggc tccgccttca
     7741 actggaggac agcctgcacc ggccacaccc agacggccca gcctgtgtag cttctgcttt
     7801 caaccactcc acccagaccc aggaaaagct gggttcctca ccctcctggg gcccccacta
     7861 tggcagtaaa tccacagaga ggctgtctag ggagcctaat ggtgtagagc cagctgagcc
     7921 aatgggaaga cgtcgagcca ggtcctcttc ggtgcctcgg gatgtaccca gactgtacct
     7981 aagttctgaa tcggagtcgc ctgcccctcg gctctcatct gagaagagca ggacctttga
     8041 ggagcacccc gaggctgcac agtgggggac aagaccacag agcagctcga agcggaggga
     8101 aagagtttcc ttccggggtc aatacacagg ccaggagtac cacattctat ccccaaaggc
     8161 catcctgaag gacagtggca caccgtcctg tccccactgc catcccatta ggacccagga
     8221 cacaggcagt gctgtctcta gagacaccac acggggctca tctgccgctg acaccctccg
     8281 ctgtgccctg tgtggtgaag tcaagtcctc tgcagaagca gatggctcca gctcaggtcc
     8341 ctctgagaag aacaccccca aaaagccttc cacccccatc ctcaaacgga agaacaggca
     8401 gacgggctcg ccagtacgaa tggcccctgg actctggtac ctggctgctg cgcccccagc
     8461 cccagcccca ccggctttgg cctacatctc ctcagctccc attatgcctt atctaccacc
     8521 cactgtgtac tatgcagccc cagcacctac ctcagcccag acagcttccc cgcagcctgc
     8581 tcggggacct cggagaaccc ggcactcagt ccaactgggt ctgaatgacc tggaggagct
     8641 gcaggccgca ctcagagagg ctgcacaggc cgccgagaac gttcgctcca ccacccgcca
     8701 gctgagccgc tccctgtctg cggacctgcg ccatgcccgt agcctgcgag gctcctgcct
     8761 tttcgtcgac ggcagcaagc ttggtggagg cagcgcatca ggcggaagcg gaagcagatc
     8821 tatggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa
     8881 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     8941 ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc
     9001 cttcgcctgg gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca
     9061 ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg
     9121 cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     9181 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     9241 gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     9301 tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc
     9361 cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt
     9421 cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     9481 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aagcggccgc
     9541 catatgcatc ctaggcctat taatattccg gagtatacgt agccggctaa cgttaacaac
     9601 cggtacctct agataagtaa tgagagctcg ctgatcagcc tcgactgtgc cttctagttg
     9661 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     9721 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     9781 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     9841 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     9901 gagatccact agttctagcc tcgaggctag agcggccgcc actggccgtc gttttacaac
     9961 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10021 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10081 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    10141 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    10201 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    10261 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    10321 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10381 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10441 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10501 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
    10561 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10621 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10681 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    10741 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    10801 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    10861 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    10921 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    10981 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11041 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11101 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11161 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11221 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11281 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11341 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11401 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11461 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    11521 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    11581 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    11641 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    11701 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    11761 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    11821 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    11881 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    11941 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    12001 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    12061 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12121 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12181 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12241 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12301 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12361 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12421 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12481 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    12541 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    12601 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    12661 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg aggcgcgccg
//
