LOCUS       sequence-408761-       10131 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 variant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Liu X, Huuskonen S, Laitinen T, Redchuk T, Bogacheva M, Salokas K, 
            Pohner I, Ohman T, Tonduru AK, Hassinen A, Gawriyski L, Keskitalo S,
            Vartiainen MK, Pietiainen V, Poso A, Varjosalo M
  TITLE     SARS-CoV-2-host proteome interactions for antiviral drug discovery.
  JOURNAL   Mol Syst Biol. 2021 Nov;17(11):e10396. doi: 10.15252/msb.202110396.
  PUBMED    34709727
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-11"; volume: "17(11):e10396. doi"; pages: " 
            10.15252/msb.202110396"
COMMENT     Sequence Label: MAC-N SARS-CoV-2 Omicron spike protein
FEATURES             Location/Qualifiers
     source          1..10131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(943..1734,1735..1803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1566..1585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1804..1908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1983..2002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2174..2553
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2554..2757
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2708..2728
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2759..2777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2780..2798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2808..2832
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3000..3350,3351..3353,3354..3962)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3963..3989
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4020..4043
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4080..4103
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    4116..4140
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7960..7984)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8022..8039)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8028..8252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8385..8404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8536..8583
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8591..9613
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8591..9613
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9743..9864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9780..9799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9834..9853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9913..9929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9913..9929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9926..9948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9937..9953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9961..9967,9968..9985,9986..9991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10006..10027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     1561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1921 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     1981 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     2041 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     2101 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     2161 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     2221 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2281 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2341 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2401 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2461 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2521 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2581 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2641 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2701 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     2761 cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg
     2821 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2881 gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga gctcggatcc
     2941 actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagacca
     3001 tgaaggacaa caccgtgccc ctgaagctga tcgccctgct ggccaacggc gagttccact
     3061 ctggcgagca gctgggcgag acactgggca tgagcagagc cgccatcaac aagcacatcc
     3121 agaccctgcg ggactggggc gtggacgtgt tcacagtgcc cggcaagggc tacagcctgc
     3181 ccgagcccat ccagctgctg aacgccaagc agatcctggg ccagctggat ggcggatctg
     3241 tggccgtgct gcccgtgatc gacagcacca accagtatct gctcgaccgg atcggcgagc
     3301 tgaagtccgg cgacgcctgt atcgccgagt accagcaggc cggcagaggc ggcaggggca
     3361 gaaagtggtt cagccccttc ggcgccaacc tgtacctgag catgttctgg agactggaac
     3421 agggcccagc cgccgctatt ggcctgagcc tggtcatcgg catcgtgatg gccgaggtgc
     3481 tgcggaagct gggcgccgac aaagtgcgcg tgaagtggcc caacgacctg tatctgcagg
     3541 accggaagct ggccggcatc ctggtggaac tgaccggcaa aacaggcgac gccgcccaga
     3601 tcgtgatcgg cgctggcatc aacatggcca tgcggcgggt ggaagagagc gtggtcaacc
     3661 agggctggat caccctgcag gaagccggca tcaacctgga ccggaacacc ctggccgcca
     3721 tgctgatcag agagctgaga gccgccctgg aactgttcga gcaggaaggc ctggccccct
     3781 acctgagcag atgggagaag ctggacaact tcatcaaccg gcccgtgaaa ctgatcatcg
     3841 gcgacaaaga gatcttcggc atcagccggg gcatcgacaa gcagggggcc ctgctcctgg
     3901 aacaggacgg catcatcaag ccctggatgg gcggcgagat cagcctgcgg agcgccgaga
     3961 agtacccata cgatgttcct gactatgccg gtaccgagct cggatccacc atggctagct
     4021 ggagccaccc gcagttcgag aaaggtggag gttccggagg tggatcggga ggtggatcgt
     4081 ggagccaccc gcagttcgaa aaagcggccg atatcacaag tttgtacaaa aaagcaggct
     4141 ccaccatgtt cgtgtttctg gtcctgctgc ctctggtctc atcacagtgc gtcaatctga
     4201 caactcggac tcagctgccc cctgcctata ctaacagctt tacacggggc gtctactatc
     4261 ccgacaaggt gttcagaagt agcgtcctgc atagcactca ggacctgttc ctgcccttct
     4321 tctccaacgt gacttgtttc catgtcatct ccgggaccaa tgggaccaaa cggtttgaca
     4381 acccagtcct gccattcaat gatggggtct attttgctag catcgaaaag agtaatatca
     4441 ttagagggtg gatctttgga accacactgg atagcaaaac ccagtccctg ctgatcgtca
     4501 acaacgcaac taacgtggtc attaaggtct gtgagttcca gttctgcaat gatcctttcc
     4561 tggaccacaa aaacaataag agttggatgg aatctgagtt cagagtctat tcaagtgcta
     4621 ataactgcac cttcgaatac gtgtcacagc cattcctgat ggatctggaa ggaaagcagg
     4681 gcaacttcaa gaatctgcgg gagtttgtct tcaagaacat cgatggatat ttcaagatct
     4741 atagcaagca tacccctatc attgtgcggg agccagaaga tctgccccag ggctttagcg
     4801 ccctggaacc tctggtcgat ctgcctatcg ggattaacat cactcggttt cagactctgc
     4861 tggccctgca tcggagttac ctgacaccag gagatagcag ttctggctgg accgccggcg
     4921 ccgctgcata ttatgtgggc tatctgcagc cacgcacatt cctgctgaaa tacaacgaaa
     4981 atgggactat tacagacgct gtcgactgcg cactggaccc tctgtcagaa acaaaatgta
     5041 ctctgaaatc tttcacagtg gaaaaaggca tctatcagac atctaacttc cgcgtgcagc
     5101 caactgaatc tatcgtgaga tttcctaata ttactaatct gtgcccattc gatgaggtgt
     5161 ttaatgctac ccggtttgcc tccgtgtatg catggaaccg gaaaaggatt agcaattgtg
     5221 tggctgatta tagcgtgctg tataatctgg cacctttctt caccttcaag tgttacggag
     5281 tgtcacccac aaagctgaat gacctgtgct ttaccaatgt ctacgcagac tctttcgtga
     5341 ttcgcggcga cgaggtgcgc cagatcgcac caggacagac cggcaatatc gcagattaca
     5401 attataagct gcctgacgac tttacagggt gcgtgatcgc ttggaacagt aacaaactgg
     5461 acagcaaagt gagcggaaac tataactatc tgtatcgcct gttcagaaaa tcaaatctga
     5521 aacccttcga aagagatatt tccactgaaa tttaccaggc aggaaataag ccctgcaacg
     5581 gggtggctgg gttcaattgt tattttccac tgagatctta ctcattccgc cccacatacg
     5641 gagtgggcca tcagccttac cgggtggtgg tgctgagctt tgaactgctg cacgcaccag
     5701 ctacagtctg cggacccaag aaatccacta acctggtcaa gaacaaatgc gtgaatttca
     5761 atttcaatgg gctgaagggc actggggtgc tgacagagtc aaataagaag tttctgcctt
     5821 tccagcagtt cggacgcgac atcgctgaca ctacagatgc agtccgggac cctcagaccc
     5881 tggagattct ggatatcact ccatgttcct tcggaggcgt ctctgtgatc acacccggaa
     5941 ctaataccag taaccaggtc gcagtgctgt accagggcgt gaactgcacc gaggtgcctg
     6001 tggccattca cgcagatcag ctgaccccca catggcgcgt ctacagtact ggctccaatg
     6061 tctttcagac tcgggcaggc tgtctgattg gagctgagta cgtgaacaac tcttacgagt
     6121 gtgacatccc aatcggagct ggcatctgtg ccagttacca gacccagacc aagagtcatc
     6181 ggcgggcccg aagtgtggct tctcagagca tcatcgccta tactatgtct ctgggcgctg
     6241 agaatagtgt ggcttactca aataatagta tcgctattcc cacaaacttc actatctctg
     6301 tgaccacaga aattctgcca gtgtcaatga ccaagacaag tgtggactgc accatgtata
     6361 tttgcggcga tagcactgag tgctcaaacc tgctgctgca gtatgggtca ttctgcaccc
     6421 agctgaaacg cgcactgaca ggcattgctg tcgagcagga caagaatacc caggaagtct
     6481 ttgcacaggt gaaacagatc tataaaacac cacctatcaa gtacttcggg ggctttaact
     6541 tctctcagat tctgcctgac ccaagcaaac catccaagag gagcttcatc gaggatctgc
     6601 tgttcaacaa agtcactctg gctgatgctg gattcattaa gcagtacgga gattgtctgg
     6661 gcgacattgc agcaagagat ctgatctgtg ctcagaagtt caagggcctg acagtcctgc
     6721 caccactgct gacagatgaa atgatcgcac agtatacttc cgccctgctg gctggcacca
     6781 tcactagcgg gtggacattc ggagccggag ctgccctgca gatccccttc gccatgcaga
     6841 tggcatacag gttcaacgga atcggggtca ctcagaatgt cctgtatgaa aatcagaagc
     6901 tgattgctaa ccagtttaat tccgccatcg gcaagattca ggattctctg agttctaccg
     6961 cctctgctct gggaaagctg caggatgtgg tcaatcataa tgcacaggca ctgaacaccc
     7021 tggtgaaaca gctgtcctct aagttcggcg ctatttcttc agtcctgaac gatattttct
     7081 ctcggctgga taaggtggag gctgaggtcc agattgatag actgatcact ggacggctgc
     7141 agtcactgca gacctacgtc actcagcagc tgattcgggc cgctgaaatc agagcatctg
     7201 caaacctggc tgccacaaaa atgagtgagt gcgtgctggg gcagagcaag agagtcgact
     7261 tctgcgggaa aggataccat ctgatgagtt tcccacagtc tgctccacat ggagtggtct
     7321 ttctgcatgt cacatacgtc cccgctcagg agaagaattt cacaactgca cccgctattt
     7381 gccacgatgg gaaggcacac tttccccgcg aaggagtctt cgtgtccaat ggaactcact
     7441 ggttcgtgac tcagcgaaac ttctacgaac cacagattat cacaactgat aatacattcg
     7501 tgtcaggcaa ttgcgacgtg gtcattggca tcgtcaacaa tacagtgtac gatcctctgc
     7561 agccagagct ggatagtttc aaagaggaac tggataaata ctttaagaac cacacctcac
     7621 ctgatgtgga cctgggggac atctctggga tcaatgcctc tgtcgtgaat attcagaagg
     7681 aaatcgaccg gctgaatgag gtcgctaaaa acctgaatga gtctctgatt gacctgcagg
     7741 agctgggcaa gtacgaacag tatatcaagt ggccatggta catctggctg gggttcatcg
     7801 caggactgat tgctatcgtg atggtgacaa tcatgctgtg ctgcatgact agttgttgct
     7861 cctgtctgaa aggctgttgt tcctgcgggt cttgttgtaa attcgacgag gatgattctg
     7921 aacccgtgct gaagggagtc aaactgcatt atacctgaga cccagctttc ttgtacaaag
     7981 tggtgacgta agctaggggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     8041 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8101 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     8161 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     8221 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     8281 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8521 tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa gtataggaac
     8581 ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     8641 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     8701 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     8761 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     8821 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     8881 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     8941 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     9001 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     9061 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     9121 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     9181 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     9241 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     9301 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     9361 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     9421 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     9481 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     9541 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     9601 ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct atgaaaggtt
     9661 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9721 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9781 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9841 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9901 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9961 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    10021 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10081 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc g
//
