LOCUS       sequence-408764-        7985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HIV Gag-delta-ZF2-MCP-P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pFH2.7_Gag-dZF2-MCP-P2A-eGFP
FEATURES             Location/Qualifiers
     source          1..7985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      923..932
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2168..2554
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             2777..2833
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2834..2836,2837..3550)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2834..3547
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2876..2897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3137..3156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3484..3505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3576..3593)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3582..3806
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3852..4280
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3939..3958)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4149..4170
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4289..4309)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4294..4623
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4474..4609
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4536..4555
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4690..5484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4744..4763)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5354..5373
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5658..5779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5695..5714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5749..5768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5828..5844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5828..5844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5841..5863)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5852..5868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5876..5882,5883..5900,5901..5906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5921..5942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6059..6076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6230..6818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6310..6329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6989..7780,7781..7849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7612..7631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7850..7954)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gggtgcgaga gcgtcagtat taagcggggg
      961 agaattagat cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1021 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1081 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1141 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1201 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1261 taagaaaaaa gcacagcaag cagcagctga cacaggacac agcaatcagg tcagccaaaa
     1321 ttaccctata gtgcagaaca tccaggggca aatggtacat caggccatat cacctagaac
     1381 tttaaatgca tgggtaaaag tagtagaaga gaaggctttc agcccagaag tgatacccat
     1441 gttttcagca ttatcagaag gagccacccc acaagattta aacaccatgc taaacacagt
     1501 ggggggacat caagcagcca tgcaaatgtt aaaagagacc atcaatgagg aagctgcaga
     1561 atgggataga gtgcatccag tgcatgcagg gcctattgca ccaggccaga tgagagaacc
     1621 aaggggaagt gacatagcag gaactactag tacccttcag gaacaaatag gatggatgac
     1681 acataatcca cctatcccag taggagaaat ctataaaaga tggataatcc tgggattaaa
     1741 taaaatagta agaatgtata gccctaccag cattctggac ataagacaag gaccaaagga
     1801 accctttaga gactatgtag accgattcta taaaactcta agagccgagc aagcttcaca
     1861 agaggtaaaa aattggatga cagaaacctt gttggtccaa aatgcgaacc cagattgtaa
     1921 gactatttta aaagcattgg gaccaggagc gacactagaa gaaatgatga cagcatgtca
     1981 gggagtgggg ggacccggcc ataaagcaag agttttggct gaagcaatga gccaagtaac
     2041 aaatccagct accataatga tacagaaagg caattttagg aaccaaagaa agactgttaa
     2101 gtgtttcaat tgtggcaaag aagggcacat agccaaaaat tgcagggccc ctaggaaaaa
     2161 gggcgttgct tcaaacttta ctcagttcgt gctcgtggac aatggtggga caggggatgt
     2221 gacagtggct ccttctaatt tcgctaatgg ggtggcagag tggatcagct ccaactcacg
     2281 gagccaggcc tacaaggtga catgcagcgt caggcagtct agtgcccaga agagaaagta
     2341 taccatcaag gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc
     2401 tgtcgccgct tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa
     2461 ttctgactgt gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat
     2521 cccttccgcc atcgccgcta actcaggtat ctacactgag agacaggcta attttttagg
     2581 gaagatctgg ccttcccaca agggaaggcc agggaatttt cttcagagca gaccagagcc
     2641 aacagcccca ccagaagaga gcttcaggtt tggggaagag acaacaactc cctctcagaa
     2701 gcaggagccg atagacaagg aactgtatcc tttagcttcc ctcagatcac tctttggcag
     2761 cgacccctcg tcacaagcta ctaacttcag cctgctgaag caggctggag acgtggagga
     2821 gaaccctgga cctatgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2881 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2941 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3001 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3061 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3121 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3181 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3241 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3301 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3361 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3421 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3481 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct
     3541 gtacaagtaa gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3601 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3661 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3721 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3781 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3841 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3901 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3961 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4021 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4081 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4141 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4201 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4261 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4321 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4381 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4441 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4501 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4561 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4621 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4681 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4741 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4801 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4861 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4921 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4981 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5041 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5101 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5161 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5221 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5281 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5341 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5401 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5461 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5521 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5581 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5641 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5701 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5761 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5821 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5881 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5941 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6001 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7021 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7081 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7381 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7441 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7921 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7981 acgtc
//
