LOCUS       sequence-408770-        8338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA expression vector for DCK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Sugita K, Onishi I, Nakayama R, Ishibashi S, Ikeda M, Inoue M, 
            Narita R, Oshima S, Shimizu K, Saito S, Sato S, Moriarity BS, 
            Yamamoto K, Largaespada DA, Kitagawa M, Kurata M
  TITLE     Indirect CRISPR screening with photoconversion revealed key factors 
            of drug resistance with cell-cell interactions.
  JOURNAL   Commun Biol. 2023 Jun 1;6(1):582. doi: 10.1038/s42003-023-04941-9.
  PUBMED    37264057
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-1"; volume: "6(1):582. doi"; pages: " 
            10.1038/s42003-023-04941-9"
COMMENT     Sequence Label: pLenti-U6-DCK-Hygro
FEATURES             Location/Qualifiers
     source          1..8338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..1310
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          363..1301
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1258..1278
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1323..2360
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             2339..2347
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    2382..2970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2435..2455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2853..2864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3042..3275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3353..3474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3390..3409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3444..3463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3514..3649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3669..3688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3670..3688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3698..3715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3698..3714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3707..3729)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3856..4311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3943..3962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4153..4174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4337..4441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4442..4510,4511..5302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4660..4679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5473..6061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5962..5981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6215..6232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6349..6370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6385..6390,6391..6408,6409..6415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6423..6439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6428..6450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6447..6463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6447..6463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6482..6502
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6484..6502
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6530..6756
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6757..6937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6984..7109
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7602..7835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     protein_bind    7914..7938
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(7982..8057)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(8079..8327)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(8137..8156)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(8307..8327)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(join(8328..8338,1..14))
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaac ttgttgatag cttaacgcta gcaaaagaaa aggggggagg gtgcaaagat
       61 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
      121 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      181 agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa
      241 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      301 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      361 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
      421 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
      481 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
      541 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
      601 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
      661 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
      721 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
      781 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
      841 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
      901 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
      961 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     1021 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     1081 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     1141 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     1201 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     1261 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacgggat
     1321 ccatgaagaa gcccgaactc accgctacca gcgttgaaaa atttctcatc gagaagttcg
     1381 acagtgtgag cgacctgatg cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg
     1441 atgtcggcgg acgcggctat gtactgcggg tgaatagctg cgctgatggc ttctacaaag
     1501 accgctacgt gtaccgccac ttcgccagcg ctgcactacc catccccgaa gtgttggaca
     1561 tcggcgagtt cagcgagagc ctgacatact gcatcagtag acgcgcccaa ggcgttactc
     1621 tccaagacct ccccgaaaca gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg
     1681 atgctattgc cgccgccgac ctcagtcaaa ccagcggctt cggcccattc gggccccaag
     1741 gcatcggcca gtacacaacc tggcgggatt tcatttgcgc cattgctgat ccccatgtct
     1801 accactggca gaccgtgatg gacgacaccg tgtccgccag cgtagctcaa gccctggacg
     1861 aactgatgct gtgggccgaa gactgtcccg aggtgcgcca cctcgtccat gccgacttcg
     1921 gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag
     1981 ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt cttctggcgg ccctggctgg
     2041 cttgcatgga gcagcagact cgctacttcg agcgccggca tcccgagctg gccggcagcc
     2101 ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca gctctaccag agcctcgtgg
     2161 acggcaactt cgacgatgct gcctgggctc aaggccgctg cgatgccatc gtccgcagcg
     2221 gggccggcac cgtcggtcgc acacaaatcg ctcgccggag cgcagccgta tggaccgacg
     2281 gctgcgtcga ggtgctggcc gacagcggca accgccggcc cagtacacga ccgcgcgcta
     2341 aggaggtagg tcgagtttaa actagtacgc gttaagtcga caatcaacct ctggattaca
     2401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     2821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2941 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     3001 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     3061 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     3121 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     3181 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     3241 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     3301 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     3361 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3421 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3481 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     3661 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     3721 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3781 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3841 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3901 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3961 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4021 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4081 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4141 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4201 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4261 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     4321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     6181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6241 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     6301 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     6361 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     6421 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     6481 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     6541 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     6601 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     6661 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     6721 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     6781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6901 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6961 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7021 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7081 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7141 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7201 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7261 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7321 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7381 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7441 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7501 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7561 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7621 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7681 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7741 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7801 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7861 ctcatttgca ccactgtccc cccttttctt ttgctagcgg gccctctaga tcaaccactt
     7921 tgtacaagaa agctgggtgg atgaatactg ccatttgtct caagatctag aattcaaaaa
     7981 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
     8041 atttctagct ctaaaaccaa attcatcttg agtacttcgg tgtttcgtcc tttccacaag
     8101 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
     8161 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     8221 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
     8281 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcagc ctgctttt
//
