LOCUS       sequence-408776-       13438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2771
FEATURES             Location/Qualifiers
     source          1..13438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4406..4417
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    9295..9883
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9348..9368)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9766..9777)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9955..10188
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10266..10387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10303..10322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10357..10376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10427..10562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10582..10601)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10583..10601)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10611..10628)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10611..10627)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10620..10642)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10769..11224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10856..10875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11066..11087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11250..11354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11355..11423,11424..12215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11573..11592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12386..12974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12875..12894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13128..13145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13262..13283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13298..13303,13304..13321,13322..13328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13336..13352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13341..13363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13360..13376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13360..13376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13395..13415
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13397..13415
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg ggcgtggggc cggggggcta
     4141 cgcggcggag ttcgtgccac cgccagagtg ccccgtcttt gagccgagtt gggaggagtt
     4201 cacagatccg ctcagcttta tcggccgcat ccggcctttg gcggagaaaa ccggcatctg
     4261 caaaattcgg ccgcccaagg actggcagcc tccatttgcc tgtgaagtaa aaagctttcg
     4321 tttcactcca agagtccagc gcctgaatga acttgaggca atgaccagag tgagattgga
     4381 tttcttggat caactagcaa aattttggga acttcaagga tctactctga agatccctgt
     4441 ggtagagaga aaaatcctgg atctgtatgc tttgagcaag attgttgcca gcaaaggagg
     4501 ttttgaaatg gtcaccaaag agaagaaatg gtctaaagtg ggtagtcgct tgggatatct
     4561 gccaggaaaa ggaactgggt ctcttttgaa gtcacattat gaaagaattc tctacccata
     4621 tgagcttttc cagtctggtg tgagccttat gggtgtgcag atgcctaatt tagatcttaa
     4681 agaaaaagtg gagcctgagg ttctcagcac tgatacccaa acttccccag agccaggcac
     4741 aaggatgaac attctgccga agagaacaag acgtgtgaag actcagtcag aatctggaga
     4801 tgtgagtaga aacacggaac tgaagaaact tcagattttt ggggctgggc ccaaggttgt
     4861 gggcttggca atgggaacaa aagataaaga agatgaggtc acccgaagac gaaaagttac
     4921 caacaggtca gacgcattta acatgcaaat gagacaacgg aaaggcactc tctctgttaa
     4981 ctttgttgat ctctatgttt gtatgttttg tggtcgggga aacaatgaag ataaattgct
     5041 tttgtgtgat ggatgtgatg acagctatca tacattttgt ctaattcctc cactacctga
     5101 tgtgcccaaa ggagactgga ggtgtcctaa atgtgtcgcc gaggaatgta gcaaacctcg
     5161 agaagccttt ggatttgaac aagctgtacg agagtataca cttcagagct ttggagagat
     5221 ggcagataat tttaagtctg attattttaa tatgccagtc catatggttc ccacagaact
     5281 agtagaaaag gaattttggc ggctggtaag cagcattgaa gaagatgtta ttgtggaata
     5341 tggagcagat atctcctcaa aagactttgg aagtggattt ccggtgaagg atgggcggag
     5401 aaagattctg ccagaagaag aggaatatgc actttctggt tggaatttga ataacatgcc
     5461 tgtcctggaa cagtctgttc ttgcacatat taatgtggac atctctggta tgaaagtgcc
     5521 gtggctctat gtgggaatgt gcttctcttc tttttgctgg cacattgagg atcactggag
     5581 ttattccatc aactacttgc actgggggga gccaaagaca tggtatggtg tgccatctca
     5641 tgctgcagag caactggagg aggtgatgag agagctggcc cccgagttat ttgaatccca
     5701 gcctgatctt ctgcatcagt tagttaccat catgaacccc aacgtgctaa tggagcatgg
     5761 tgtgcctgtg tacaggacca atcagtgtgc tggcgagttt gttgtgacat ttcctcgtgc
     5821 ctatcactct ggatttaacc agggctacaa ctttgctgaa gctgtgaact tctgtactgc
     5881 tgactggttg cccattggac gtcaatgtgt aaatcattac cgacgcctaa ggcgccactg
     5941 tgtcttttca cacgaggaac taattttcaa gatggcagca gatccagaat gcttagatgt
     6001 ggggctggct gccatggtct gcaaagaatt gactctcatg actgaagaag aaacacgatt
     6061 aagagagtct gttgtacaga tgggtgtcct gatgtcagaa gaagaagtgt ttgaacttgt
     6121 tcctgatgat gagcggcagt gttcagcatg cagaaccaca tgttttctct ctgctctcac
     6181 atgttcctgt aatcctgagc ggcttgtatg tctctaccat ccaactgatc tgtgcccctg
     6241 ccccatgcag aagaaatgtc ttagatatcg ctacccatta gaagacctcc cttctctgct
     6301 atatggtgta aaagtcaggg cacagtccta tgacacttgg gtcagtcgtg ttacagaagc
     6361 attgtctgct aacttcaacc acaaaaaaga tttgattgaa ttgcgagtaa tgctggaaga
     6421 tgctgaggat aggaaatacc cagagaatga tctctttcga aaactcaggg atgctgtaaa
     6481 agaagctgag acctgtgctt ctgtggctca gctgcttctg agcaaaaagc agaaacacag
     6541 acagagccca gatagtggga ggactcggac caaactgaca gtggaagaat tgaaggcctt
     6601 tgtccaacaa ctttttagtc ttccgtgtgt catcagccaa gctcggcaag taaagaatct
     6661 gctagatgat gtggaagagt ttcatgaacg tgctcaggag gccatgatgg atgaaacccc
     6721 agattcttcc aaactccaga tgttgataga tatgggctct agtctctatg tggaactccc
     6781 tgaattacca cgactgaagc aagagctaca acaggctcgg tggttggacg aagtaagact
     6841 gaccttatca gatccgcaac aagtcacttt ggatgtcatg aagaagctga tagactctgg
     6901 ggtagggttg gcaccccacc atgctgtgga gaaagcaatg gctgaactac aggagctcct
     6961 tacagtctct gaacgatggg aagaaaaggc taaggtctgc ctacaggcaa gaccgaggca
     7021 cagtgtggca agtttagaaa gcattgtgaa tgaagccaag aacattccag cctttctacc
     7081 caatgtgttg tccttgaaag aagccttaca aaaggctcga gaatggaccg ctaaagtgga
     7141 agctattcag agtggcagca attacgctta tttggagcag cttgagagct tgtctgcgaa
     7201 aggacgccct attcctgtgc gtcttgaagc actgccgcaa gtggaatcac aggtagcagc
     7261 agcacgggca tggagagaac ggactgggcg gacgtttctt aagaagaatt ctagccatac
     7321 attgttacag gtgctgagcc cccggaccga cattggtgta tatgggagtg gcaaaaatag
     7381 gaggaaaaaa gtaaaagaac taatagaaaa agaaaaagaa aaggatctgg acctggagcc
     7441 tctgagtgat ctggaggaag gattggagga aaccagagat acagccatgg tggtggcagt
     7501 tttcaaagaa cgggagcaaa aagagattga agccatgcat tctctcagag cagccaacct
     7561 agccaagatg acaatggtgg accgcataga agaagtaaaa ttttgcattt gccgcaagac
     7621 agccagtggg tttatgctac agtgtgagct ctgcaaagac tggttccata acagctgtgt
     7681 tcctcttcct aaatcaagtt cccaaaaaaa aggatccagc tggcaagcta aagaagtaaa
     7741 attcctttgc cctctttgta tgcggtctcg aaggcccagg ctagagacta ttctgtcact
     7801 cctggtatcc cttcagaagt tgcccgtacg gttgcctgaa ggagaggccc tgcagtgttt
     7861 gacagaacgt gctatgagtt ggcaagatag agcgcggcag gctctagcca cagatgaact
     7921 atcctctgcc ctggccaaac tatctgtgtt gagccagcgt atggtggaac aggcggctcg
     7981 agaaaaaact gaaaagatca tcagtgcaga actccaaaaa gcagctgcca atccagactt
     8041 acagggacac ttacctagtt tccagcagtc tgcttttaac cgggtggtga gcagtgtgtc
     8101 atcttctcct cgacaaacaa tggactatga tgatgaagaa acagactctg atgaagacat
     8161 tcgagagaca tatggctacg acatgaagga cacagccagt gtgaagtcct ctagtagtct
     8221 tgaacccaat cttttttgtg atgaagagat tcccatcaaa tccgaggagg tggtgaccca
     8281 catgtggaca gcaccttcat tttgtgcaga gcatgcttat tcttctgctt ctaagagttg
     8341 ttctcaaggt tctagcaccc caaggaaaca acctcggaag agccctttgg tgccccgaag
     8401 tttggaacct ccagtgctgg agttgtcacc tggagctaag gcacaactgg aagaacttat
     8461 gatggttgga gatctcctgg aagtatctct ggacgagact caacacatat ggcggatttt
     8521 gcaggccaca cacccaccct ctgaagacag attcttgcat atcatggagg atgacagcat
     8581 ggaagagaaa ccactaaaag tgaaaggaaa ggactcttca gagaagaaac ggaaacggaa
     8641 gctagaaaag gtagagcaac tttttggaga aggaaaacag aagtccaagg agttaaagaa
     8701 aatggacaaa cctagaaaga agaaattaaa attaggtgca gacaaatcaa aggagctgaa
     8761 taaactggcc aagaaactag caaaagaaga agagagaaag aaaaagaagg agaaggctgc
     8821 tgcagccaaa gttgaacttg tgaaagagag cactgaaaag aaaagagaga aaaaggtgct
     8881 ggacatcccc tcaaagtatg actggtcagg agcagaggag tctgatgatg agaatgctgt
     8941 gtgcgcagca cagaactgcc aaaggccctg caaggacaag gtagactggg tacaatgtga
     9001 tggtggctgt gatgagtggt ttcatcaagt ttgtgtgggt gtatctccag aaatggctga
     9061 aaatgaagat tacatctgta taaactgtgc aaagaagcag gggccagtta gcccaggtcc
     9121 agcaccacct ccttccttca taatgagcta caaactacca atggaggatc ttaaagagac
     9181 cagttagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     9241 ttgtggaaag gacgaagccg gcacgacact cctggaggca cgcgttaagt cgacaatcaa
     9301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9421 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9481 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9541 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9601 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9661 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9721 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9781 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9841 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9961 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
    10021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
    10081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
    10141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
    10201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
    10261 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10381 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
    10441 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10501 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10561 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    10621 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    10681 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    10741 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
    10801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10981 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    11041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    11101 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    11161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    11221 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    11281 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    11341 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    11401 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    11461 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    11521 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    11581 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    11641 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    11701 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    11761 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    11821 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11881 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11941 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    12001 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    12061 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    12121 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    12181 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    12241 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    12301 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    12361 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    12421 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    12481 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    12541 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    12601 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    12661 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    12721 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    12781 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12841 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12901 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12961 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    13021 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    13081 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    13141 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    13201 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    13261 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    13321 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    13381 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
