LOCUS       sequence-408789-        5160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-NTRK1
FEATURES             Location/Qualifiers
     source          1..5160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(92..119)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(211..297)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     346..368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     360..377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     361..377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    393..492
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2863..2962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2979..2998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2980..2998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3003..3019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3003..3019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3450..4241
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4334..4922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4823..4842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5076..5093
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
       61 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      121 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
      181 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      241 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
      301 cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt
      361 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
      421 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      481 aaaaagttgg caccatgctg cgaggcggac ggcgcgggca gcttggctgg cacagctggg
      541 ctgcggggcc gggcagcctg ctggcttggc tgatactggc atctgcgggc gccgcaccct
      601 gccccgatgc ctgctgcccc cacggctcct cgggactgcg atgcacccgg gatggggccc
      661 tggatagcct ccaccacctg cccggcgcag agaacctgac tgagctctac atcgagaacc
      721 agcagcatct gcagcatctg gagctccgtg atctgagggg cctgggggag ctgagaaacc
      781 tcaccatcgt gaagagtggt ctccgtttcg tggcgccaga tgccttccat ttcactcctc
      841 ggctcagtcg cctgaatctc tccttcaacg ctctggagtc tctctcctgg aaaactgtgc
      901 agggcctctc cttacaggaa ctggtcctgt cggggaaccc tctgcactgt tcttgtgccc
      961 tgcgctggct acagcgctgg gaggaggagg gactgggcgg agtgcctgaa cagaagctgc
     1021 agtgtcatgg gcaagggccc ctggcccaca tgcccaatgc cagctgtggt gtgcccacgc
     1081 tgaaggtcca ggtgcccaat gcctcggtgg atgtggggga cgacgtgctg ctgcggtgcc
     1141 aggtggaggg gcggggcctg gagcaggccg gctggatcct cacagagctg gagcagtcag
     1201 ccacggtgat gaaatctggg ggtctgccat ccctggggct gaccctggcc aatgtcacca
     1261 gtgacctcaa caggaagaac ttgacgtgct gggcagagaa cgatgtgggc cgggcagagg
     1321 tctctgttca ggtcaacgtc tccttcccgg ccagtgtgca gctgcacacg gcggtggaga
     1381 tgcaccactg gtgcatcccc ttctctgtgg atgggcagcc ggcaccgtct ctgcgctggc
     1441 tcttcaatgg ctccgtgctc aatgagacca gcttcatctt cactgagttc ctggagccgg
     1501 cagccaatga gaccgtgcgg cacgggtgtc tgcgcctcaa ccagcccacc cacgtcaaca
     1561 acggcaacta cacgctgctg gctgccaacc ccttcggcca ggcctccgcc tccatcatgg
     1621 ctgccttcat ggacaaccct ttcgagttca accccgagga ccccatccct gacactaaca
     1681 gcacatctgg agacccggtg gagaagaagg acgaaacacc ttttggggtc tcggtggctg
     1741 tgggcctggc cgtctttgcc tgcctcttcc tttctacgct gctccttgtg ctcaacaaat
     1801 gtggacggag aaacaagttt gggatcaacc gcccggctgt gctggctcca gaggatgggc
     1861 tggccatgtc cctgcatttc atgacattgg gtggcagctc cctgtccccc accgagggca
     1921 aaggctctgg gctccaaggc cacatcatcg agaacccaca atacttcagt gatgcctgtg
     1981 ttcaccacat caagcgccgg gacatcgtgc tcaagtggga gctgggggag ggcgcctttg
     2041 ggaaggtctt ccttgctgag tgccacaacc tcctgcctga gcaggacaag atgctggtgg
     2101 ctgtcaaggc actgaaggag gcgtccgaga gtgctcggca ggacttccaa cgtgaggctg
     2161 agctgctcac catgctgcag caccagcaca tcgtgcgctt cttcggcgtc tgcaccgagg
     2221 gccgccccct gctcatggtc tttgagtata tgcggcacgg ggacctcaac cgcttcctcc
     2281 gatcgcatgg acccgatgcc aagctgctgg ctggtgggga ggatgtggct ccaggccccc
     2341 tgggtctggg gcagctgctg gccgtggcta gccaggtcgc tgcggggatg gtgtacctgg
     2401 cgggtctgca ttttgtgcac cgggacctgg ccacacgcaa ctgtctagtg ggccagggac
     2461 tggtggtcaa gattggtgat tttggcatga gcagggatat ctacagcacc gactattacc
     2521 gtgtgggagg ccgcaccatg ctgcccattc gctggatgcc gcccgagagc atcctgtacc
     2581 gtaagttcac caccgagagc gacgtgtgga gcttcggcgt ggtgctctgg gagatcttca
     2641 cctacggcaa gcagccctgg taccagctct ccaacacgga ggcaatcgac tgcatcacgc
     2701 agggacgtga gttggagcgg ccacgtgcct gcccaccaga ggtctacgcc atcatgcggg
     2761 gctgctggca gcgggagccc cagcaacgcc acagcatcaa ggatgtgcac gcccggctgc
     2821 aagccctggc ccaggcacct cctgtctacc tggatgtcct gggcccaact ttcttgtaca
     2881 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2941 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     3001 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3061 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga
     3121 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     3181 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     3241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     3301 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     3361 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     3421 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     3481 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     3541 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     3601 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     3661 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     3721 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     3781 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     3841 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     3901 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     3961 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     4021 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     4081 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     4141 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     4201 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     4261 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     4321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4441 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5041 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5101 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
//
