LOCUS       sequence-408793-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   AlHarbi S, Frokjaer-Jensen C
  TITLE     Characterizing a standardized BioPart for PVQ-specific expression in
            C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 22;2023:10.17912/micropub.biology.000870. 
            doi: 10.17912/micropub.biology.000870. eCollection 2023.
  PUBMED    37426742
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-22"; volume: 
            "2023:10.17912/micropub.biology.000870. doi"; pages: " 
            10.17912/micropub.biology.000870. eCollection 2023"
COMMENT     Sequence Label: pJRM4 - [BAGp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1894..1910)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1894..1910)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1907..1929)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2082..2670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2162..2181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2787..2811)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2911..3702,3703..3771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3534..3553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3772..3876)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3975..3997
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3989..4006
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3990..4006
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4630..4663)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gatatcaaaa atgattacgc caagctgtaa gtttaaacat gatcttacta actaactatt
       61 ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta
      121 atgcctttct cagaagcctc taagtttccc gtgctttctt gtcaaattga ttaggaaatg
      181 attcggattt tctgaatttt tgaaagattt ttcggttttt tcagtgattt caatcggaaa
      241 ttcggagccg gaaatgcatt aggaaggggc taatagctat aaattgacac gggaaattca
      301 gatttttcat tcatttttca cacattttct gttacttttt ctcaatgatt tttttgtgtt
      361 tccatttttt ttcctttaaa aaaattctgt gtggaaatat tttccgcaaa actttatttt
      421 taaaaagaga ccaaaaatgg ggccctctaa gggcgaagaa ttgttcacgg gagtagtccc
      481 aatcttagtc gagcttgacg gaggtgagtt ttattatcga ttttcgggat tttctacttg
      541 taattacctt ttgtttttaa taagttgctt ttttccagat gttaatggcc acaagttttc
      601 cgtgtctgga gaaggagaag gcgacgctac ctacggtaag ctgaccctga agttcatttg
      661 cactactggc aagttaccag taccatggcc gaccctggtg acaacatttt gctacggggt
      721 tcaatgcttc tccagatacc caggtaggtt ttcagtttca atttttgttg caactacctc
      781 aactctatgt tttcagatca tatgaaaaga cacgactttt ttaaatccgc catgccagag
      841 ggttacgttc aagaacgtac catttttttt aaggacgatg gtaactacaa gacacgtgcg
      901 gaggttaaat ttgaaggcga tactctcgtg aaccgtattg agctcaaggt aaatttttca
      961 agcttctaaa ttccaaaata aaccatttta atttcttaat tccagggaat cgatttcaag
     1021 gaagatggaa acattttggg ccacaagctt gaatataatt ataatagtca taatgtctac
     1081 attatggccg acaaacaaaa aaacggtatt aaggttaact tcaagattcg ccacaatatc
     1141 gaagatggat ctgtccaact tgctgaccac taccaacaaa atactccaat tggagatgga
     1201 ccggttttgc ttccagataa ccactacctt tccactcagt ctgccctctc taaggatccg
     1261 aatgaaaaac gtgaccatat ggttcttttg gagttcgtta ccgcagcagg aattactcac
     1321 ggaatggatg aactgtacaa gcccgggtga tggtctctga gctatgcaaa atcctttcaa
     1381 gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt
     1441 atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct
     1501 gagtgaagtg aatgctatca aaatgtgatt ctgatatcca ggaaacggag aatctgtgcg
     1561 gcaatgtctt atcatttgaa acgcgccgta tcgatgctga catcatgcgg ctcccgtgtt
     1621 agtcgagaag atgggtacgg gcctttattc atccaacact ctacggcttc ttcaagagct
     1681 agtagggcac cctgtagttg gaaagggatt atttcgtatg gcgagcccat accgtctttc
     1741 ttgcggaagc ttaacacgat aggaagttgg atagtttcga acgatggttc catacggaac
     1801 gctgtctcaa ggatgagtct gacggagtgt aactcgaagg ctaggtggag gctcagtgat
     1861 gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg
     1921 ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg
     1981 tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag
     2041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2701 gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc
     2761 aaatcgtcct cgtgatacca attcggagcc tgcttttttg tacaaacttg ttgataatgg
     2821 caattcaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2881 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3001 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagagccacg
     3061 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3781 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3841 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccaga
     3901 tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca
     3961 agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc
     4021 acgcatctgg aataaggaag tgccattccg cctgacctaa gtgcttttcg agatcgccaa
     4081 accggagaac gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta
     4141 tagatttgct ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt
     4201 aggagacatc ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct
     4261 tctgaagtgt tttgactttg aattcggatt ctgtattccg aattcacgaa acaactgtga
     4321 acatatctat gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt
     4381 acaattcaaa agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac
     4441 tcgttctgac agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta
     4501 ctcatatgat gcataaatct tcattgttga gtttatcttg ttgatttttg aataaattat
     4561 caactcttta ctttttaatg ggttatgaaa taaataaaca ttgaaaactg ataaacaacg
     4621 ttcatctcta taacttcgta tagcatacat tatacgaagt tat
//
