LOCUS       sequence-408832-       11771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For multiplex plant prime editing in wheat plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11771)
  AUTHORS   Ni P, Zhao Y, Zhou X, Liu Z, Huang Z, Ni Z, Sun Q, Zong Y
  TITLE     Efficient and versatile multiplex prime editing in hexaploid wheat.
  JOURNAL   Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1.
  PUBMED    37386475
REFERENCE   2  (bases 1 to 11771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2023-06-29"; volume: "24(1):156. doi"; pages: " 
            10.1186/s13059-023-02990-1"
COMMENT     Sequence Label: CMPE-ePPEplus
FEATURES             Location/Qualifiers
     source          1..11771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2575..2631
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2635..2661
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2701..2721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             6735..6743
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7045..7065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8791..8811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8871..9047
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9601..9617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9601..9617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9614..9636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9625..9641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9649..9655,9656..9673,9674..9679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9694..9715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9832..9849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10003..10591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10083..10102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10762..11553,11554..11622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11385..11404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11623..11727)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cccctctcta
     1021 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1081 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1141 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1201 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1261 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1321 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1381 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1441 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     1501 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     1561 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     1621 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     1681 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     1741 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     1801 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     1861 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     1921 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     1981 tttggtgtta cttctgcaaa gcttatggac cactacctcg acatcaggct caggccagac
     2041 ccagagttcc caccagccca gctcatgtcc gtcctcttcg gcaagctcca ccaggccctc
     2101 gtggcccagg gcggcgacag gatcggcgtg tccttcccag acctcgacga gtccaggtcc
     2161 aggctcggcg agaggctccg catccacgcc tccgccgacg acctcagggc cctcctcgcc
     2221 aggccgtggc tggagggcct cagggaccac ctccagttcg gcgagccagc cgtggtgcca
     2281 cacccaaccc catacaggca agtgtccagg gtgcaagcca agtccaaccc agagaggctc
     2341 aggaggaggc tcatgaggag gcacgacctc tccgaggaag aggccaggaa gcgcatccca
     2401 gacaccgtgg ccagggccct cgacctccca ttcgtgaccc tcaggtccca gtccaccggc
     2461 cagcacttcc gcctcttcat caggcacggc ccactccagg tgaccgccga ggagggcggc
     2521 tttacctgct acggcctctc caagggcggc ttcgtgccgt ggttcggctc cggcgccacc
     2581 aacttctccc tcctcaagca agccggcgac gtggaggaga acccaggccc aatgccagcc
     2641 gccaagcgcg tcaagctgga cggcggaaag cgcaccgccg acggatccga gttcgagagc
     2701 cctaagaaaa agagaaaagt ggacaagaag tactcgatcg gcctcgatat tgggactaac
     2761 tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc
     2821 ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct cctgttcgac
     2881 agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc
     2941 aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc gaaggttgac
     3001 gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa gaagcacgag
     3061 cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa gtaccctacg
     3121 atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct ccgcctgatc
     3181 tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga gggggatctc
     3241 aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac gtacaaccag
     3301 ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct
     3361 aggctctcga agtctcggaa gctcgagaac ctgatcgccc agctgccggg cgagaagaag
     3421 aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa cttcaagtcg
     3481 aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata cgacgatgac
     3541 ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct cgctgccaag
     3601 aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga gattacgaag
     3661 gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga tctgaccctc
     3721 ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt cttcgatcag
     3781 tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga gttctacaag
     3841 ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt gaagctcaag
     3901 cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc acaccagatt
     3961 catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc tttcctcaag
     4021 gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta cgtcggccca
     4081 ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga gacgatcacg
     4141 ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg
     4201 atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca ctcgctcctg
     4261 tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac cgagggcatg
     4321 aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct cctgttcaag
     4381 accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa gattgagtgc
     4441 ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct ggggacctac
     4501 cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga gaatgaggat
     4561 atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat gatcgaggag
     4621 cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct caagaggagg
     4681 aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg ggacaagcag
     4741 tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg caatttcatg
     4801 cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc tcaggtgagc
     4861 ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc agctatcaag
     4921 aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac
     4981 aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca gaagggccag
     5041 aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct ggggtcccag
     5101 atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct ctacctgtac
     5161 tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa caggctcagc
     5221 gattacgacg tcgatgccat cgttccacag tcattcctga aggatgactc cattgacaac
     5281 aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc ttcagaggag
     5341 gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct gatcacgcag
     5401 cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct ggacaaggcg
     5461 ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt
     5521 ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg cgaggtgaag
     5581 gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca gttctacaag
     5641 gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc
     5701 acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg ggactacaag
     5761 gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa ggctaccgcc
     5821 aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac tctggccaat
     5881 ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga gatcgtgtgg
     5941 gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca agtgaatatc
     6001 gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct gcctaagcgg
     6061 aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta cggcgggttc
     6121 gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc
     6181 aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg ctccagcttc
     6241 gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa gaaggacctg
     6301 atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa gcggatgctg
     6361 gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa gtatgtgaac
     6421 ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga caacgagcag
     6481 aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga gcagatttcc
     6541 gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct ctccgcgtac
     6601 aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca tctcttcacc
     6661 ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat cgatcgcaag
     6721 aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc gattaccggc
     6781 ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acgaattctc cgggagcgag
     6841 acgccaggca cctccgagtc ggccacccca gaatctgcca cagtggtgtc cggccaaaag
     6901 caggaccgcc agggcggaga acgcagaagg tcccagctcg atagggatca gtgtgcctac
     6961 tgcaaggaga agggccactg ggccaaagac tgcccgaaaa agccgcgcgg cccacgcggc
     7021 ccaaggccac aaacatccct ccttccaaag aagaagcgga aggtggagct cagcggagga
     7081 tcttccggag gatctagcgg ctccgagaca ccaggaacat ccgaaagcgc tacaccagaa
     7141 tctagcggag gctcttccgg aggatctagg cctaccctca acatcgagga tgagtatcgc
     7201 ctccacgaaa cctccaaaga accggacgtg tccctcggca gcacatggct cagcgacttc
     7261 ccacaagcgt gggccgaaac cggcggcatg ggcctcgccg tccgccaagc cccactcatt
     7321 atcccgctga aggcgacctc cacaccggtg tccatcaagc agtacccgat gagccaagag
     7381 gcgaggctcg ggattaagcc gcacattcag cgcctcctcg atcaaggcat tctcgtgccg
     7441 tgccaatccc cgtggaatac accactcctc ccggtcaaaa agccgggcac caacgactat
     7501 cgcccggtcc aagatctccg cgaggtcaac aagcgcgtgg aagacatcca cccgaccgtc
     7561 ccgaacccgt ataatctgct ctccgggctc ccaccatccc accagtggta tacagtgctg
     7621 gacctcaaag acgccttctt ctgtctccgc ctccacccaa caagccagcc gctcttcgcc
     7681 ttcgagtggc gcgacccgga gatgggcatc tccggccaac tgacatggac acgcctcccg
     7741 caaggcttca agaacagccc gacactcttc aacgaggcgc tccataggga cctcgcggat
     7801 tttcgcatcc agcatccgga cctcatcctc ctccagtatg ccgatgatct cctcctcgcc
     7861 gcgacctccg agctggattg tcaacaaggc acacgcgcgc tcctccaaac actcgggaac
     7921 ctcggctatc gcgcgtccgc gaaaaaggcc caaatctgcc agaagcaagt gaagtacctc
     7981 gggtatctgc tcaaggaagg ccaacgctgg ctcaccgaag cgcgcaaaga aacagtgatg
     8041 gggcaaccga caccgaaaac accacgccag ctgcgcgagt ttctcggcaa agccggcttc
     8101 tgtcgcctct tcatcccggg ctttgccgag atggccgcgc cactctaccc actcaccaag
     8161 ccgggcacac tgtttaactg ggggccggat cagcagaaag cctaccaaga gatcaaacaa
     8221 gcgctcctca ccgccccagc gctcgggctc ccagatctca caaagccgtt cgagctgttc
     8281 gtcgatgaga agcaaggcta cgcgaagggc gtgctcacac agaagctcgg cccgtggagg
     8341 aggccagtgg cctatctctc caaaaaactc gatccagtgg ccgccggctg gccaccgtgt
     8401 ctgcgcatgg tcgccgcgat tgccgtgctc acaaaggatg ccggcaaact cacaatgggc
     8461 cagccgctgg tgatcctcgc gccacatgcc gtggaagccc tcgtcaaaca gccgccggat
     8521 aggtggctct ccaatgcgcg catgacccat taccaagcgc tcctcctcga caccgatcgc
     8581 gtccagttcg gcccagtggt cgccctcaat ccggcgacac tgctgccact cccagaggag
     8641 ggcctccaac acaactgtct ggatattctc gcggaagcgc atggcacaag gccagacctc
     8701 acagatcaac cgctcagcgg cggcagcaaa aggaccgccg attcccaaca ttccacccca
     8761 ccaaaaacca agaggaaggt gggctctggc ccaaagaaga agaggaaggt ctgagcggcc
     8821 gcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     8881 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     8941 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     9001 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc taaagtccct
     9061 atagatcttt gtcgtgaata taaaccagac acgagacgac taaacctgga gcccagacgc
     9121 cgttcgaagc tagaagtacc gcttaggcag gaggccgtta gggaaaagat gctaaggcag
     9181 ggttggttac gttgactccc ccgtaggttt ggtttaaata tgatgaagtg gacggaagga
     9241 aggaggaaga caaggaagga taaggttgca ggccctgtgc aaggtaagaa gatggaaatt
     9301 tgatagaggt acgctactat acttatacta tacgctaagg gaatgcttgt atttataccc
     9361 tataccccct aataacccct tatcaattta agaaataatc cgcataagcc cccgcttaaa
     9421 aattggtatc agagccatga ataggtctat gaccaaaact caagaggata aaacctcacc
     9481 aaaatacgaa agagttctta actctaaaga taaaagatct ttcaagatca aaactagttc
     9541 cctcacaccg gtgacgggga tcgcatgcga tatctcgaga tctagcttgg cgtaatcatg
     9601 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9661 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10021 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10081 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10141 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10201 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10261 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10321 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10381 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10441 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10501 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10561 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10621 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10681 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10741 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10801 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10861 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10921 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10981 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11041 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11101 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11161 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11221 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11281 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11341 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11401 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11461 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11521 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11581 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11641 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11701 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgccagtgc
    11761 caagctaatt c
//
