LOCUS       sequence-408836-       12038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 12038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pSLQ9713:
            pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-...
FEATURES             Location/Qualifiers
     source          1..12038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        209..708
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(244..267)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     641..660
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2400..2426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2406..2432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3069..3095
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3108..3815
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3250..3268)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3518..3537
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3879..4467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3932..3952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4350..4361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4470..4486)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4471..4487)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4577..4810
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4835..4853)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4836..4853)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5143..5162)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5376..5395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5495..5517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5555..5573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5641..5745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5746..5814,5815..6606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5964..5983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6777..7365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7266..7285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7519..7536
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7606..7626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7611..7940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7791..7926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9074..9139
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9269..9289
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9714..9848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9751..9770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9805..9824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10016..10649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10696..10821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11318..11551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11736..11780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      181 atcgataagc ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      241 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      301 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      361 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      421 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      481 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      541 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
      601 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
      661 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
      721 agcttacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccgggcc accatgggac
      781 ccaagaaaaa acgcaaggtg ggaagcggat ccgccaaaaa caccattacc aaaacactga
      841 aactgcgtat tgtgcgtccg tataatagcg cagaagtgga aaaaattgtt gccgacgaaa
      901 aaaacaaccg cgaaaaaatc gcactggaaa agaacaaaga caaagtgaaa gaagcctgca
      961 gcaaacatct gaaagttgca gcatattgta ccacacaggt tgaacgtaat gcatgcctgt
     1021 tttgtaaagc acgtaaactg gatgacaaat tctaccaaaa actgcgtggt cagtttccgg
     1081 atgcagtttt ttggcaagaa atcagcgaaa tttttcgcca gctgcagaaa caggcagcag
     1141 aaatctataa tcagagcctg atcgaactgt actacgagat ttttatcaaa ggcaaaggta
     1201 ttgcaaatgc cagcagcgtt gaacattatc tgagtagagt ttgttataga cgtgcagcag
     1261 aactgtttaa aaacgcagca attgcaagcg gtctgcgtag caaaatcaaa agcaattttc
     1321 gtctgaaaga actgaaaaac atgaaaagtg gtctgccgac caccaaaagc gataattttc
     1381 cgattccgct ggttaaacag aaaggtggtc agtataccgg ttttgaaatt agcaatcata
     1441 atagcgactt catcatcaag attccgtttg gtcgttggca ggtcaaaaaa gagattgata
     1501 aatatcgtcc gtgggagaaa tttgactttg aacaggttca gaaaagcccg aaaccgatta
     1561 gcctgctgct gagcacccag cgtcgtaaac gtaataaagg ttggagcaaa gatgaaggca
     1621 ccgaagccga aatcaaaaaa gttatgaatg gcgattatca gaccagctac attgaagtta
     1681 aacgtggcag caaaatctgt gaaaaaagcg catggatgct gaatctgagc attgatgttc
     1741 cgaaaattga taaaggtgtg gatccgagca ttattggtgg tattgcagtt ggtgttagat
     1801 caccgctggt ttgcgcaatt aacaatgcat ttagccgtta tagcatcagc gataacgacc
     1861 tgtttcactt caacaagaaa atgtttgcac gtcgtcgtat cctgctgaaa aaaaaccgtc
     1921 ataaacgtgc aggtcatggt gcaaaaaaca aactgaaacc gatcaccatt ctgaccgaaa
     1981 aaagtgaacg ttttcgcaaa aagctgattg aacgttgggc atgtgaaatc gcggatttct
     2041 tcattaaaaa caaagttggc accgtgcaga tggaaaatct ggaaagcatg aaacgtaaag
     2101 aggacagcta ttttaacatt cgcctgcgtg gcttttggcc gtatgcagaa atgcagaaca
     2161 aaatcgaatt caaactgaag cagtatggca tcgaaattcg taaagttgca ccgaataata
     2221 ccagcaaaac ctgtagcaaa tgtggccatc tgaacaacta tttcaacttc gagtaccgca
     2281 agaaaaacaa attcccgcac tttaaatgcg aaaaatgcaa cttcaaagaa aacgccgcgt
     2341 ataatgcagc cctgaatatt tcaaacccga aactgaaaag caccaaagag agaccggcct
     2401 atccctatga cgtgcccgat tatgccagcc tgggcagcgg cgacggcatt ggtagtggga
     2461 gcaacggcag cagcctcagt ccgttttccc ctgttcaact ccatcaactt cgggcgcaaa
     2521 ttcttgcata taaaatgctc gctcgtggtc aaccgttgcc agaaactctt caacttgcag
     2581 tccagggtgg cggtggtggc tccggcgaag gtcaatccga tgaacgcgca cttcttgatc
     2641 aacttcatac attgcttagt aatacagatg caactggtct cgaagaaatt gatcgggcgc
     2701 tcgggatacc tgaacttgtc aatcaaggtc aagcacttga acctaaacaa ggcggcggtg
     2761 gatcaggttt tgttacattg aaagatgtag ggatggattt tacactcggg gattgggaac
     2821 aattggggct cgaacaaggg gatacgtttt gggatactgc tctcgataat tgtcaagatc
     2881 tctttctcct cgatccacct ggcggcggtg ggtctggcca tgagaaattt ccgtctgatc
     2941 tcgatttgga tatgtttaat gggtcactcg aatgtgatat ggaatcaatt attcgcagtg
     3001 aactcatgga tgcagatgga ctcgatttta attttgattc acatatgggt ggaggctccg
     3061 gggaagatcc tgctgccaaa cgcgttaaac tagacatggg aagcggagtg agcaagggcg
     3121 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     3181 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3241 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     3301 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     3361 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3421 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     3481 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3541 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3601 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3661 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3721 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3781 actccaccgg cggcatggac gagctgtaca agtaagcggc cgcataagac tctagagtcg
     3841 acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     3901 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3961 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4021 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4081 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4141 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4201 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4261 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     4321 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4381 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4441 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaggg aattaattcg
     4501 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4561 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4621 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4681 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4741 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4801 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     4861 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     4921 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     4981 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     5041 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     5101 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     5161 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     5221 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     5281 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     5341 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5521 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5581 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5641 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5701 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5761 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5821 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5881 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5941 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6001 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6061 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6121 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6181 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6241 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6301 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6361 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6421 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6481 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6541 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6601 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6661 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6721 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7441 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7501 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7561 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     7621 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7681 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7741 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7921 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     7981 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     8041 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     8101 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     8161 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     8221 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     8281 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     8341 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     8401 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     8461 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     8521 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     8581 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     8641 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     8701 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     8761 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     8821 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     8881 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     8941 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     9001 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     9061 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     9121 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     9181 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     9241 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     9301 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     9361 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     9421 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     9481 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     9541 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     9601 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9661 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9721 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9781 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9841 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9901 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     9961 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
    10021 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
    10081 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
    10141 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
    10201 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
    10261 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
    10321 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
    10381 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
    10441 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    10501 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10561 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10621 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    10681 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    10741 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    10801 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    10861 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    10921 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    10981 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    11041 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    11101 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    11161 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    11221 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    11281 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    11341 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    11401 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    11461 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    11521 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    11581 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11641 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11701 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11761 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11821 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    11881 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    11941 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
    12001 acagatccat tcgattagtg aacggatctc gacggtat
//
