LOCUS       sequence-408839-       11909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Tycko J, Van MV, Aradhana, DelRosso N, Ye H, Yao D, Valbuena R, 
            Vaughan-Jackson A, Xu X, Ludwig C, Spees K, Liu K, Gu M, Khare V, 
            Mukund AX, Suzuki PH, Arana S, Zhang C, Du PP, Ornstein TS, Hess GT,
            Kamber RA, Qi LS, Khalil AS, Bintu L, Bassik MC
  TITLE     Development of compact transcriptional effectors using 
            high-throughput measurements in diverse contexts.
  JOURNAL   Nat Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6.
  PUBMED    39487265
REFERENCE   2  (bases 1 to 11909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Nov 1. doi: 10.1038/s41587-024-02442-6."
COMMENT     Sequence Label: pSLQ9715:
            pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-M...
FEATURES             Location/Qualifiers
     source          1..11909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        209..708
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(244..267)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     641..660
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             780..800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2400..2426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2406..2432
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             2940..2966
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2979..3686
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3121..3139)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3389..3408
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3750..4338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3803..3823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4221..4232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4341..4357)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4342..4358)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4448..4681
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4706..4724)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4707..4724)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5014..5033)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5247..5266)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5366..5388
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5426..5444)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5512..5616
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5617..5685,5686..6477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5835..5854)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6648..7236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7137..7156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7390..7407
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7477..7497)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7482..7811
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7662..7797
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8945..9010
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9140..9160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9585..9719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9622..9641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9676..9695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9887..10520
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10567..10692
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11189..11422
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11607..11651
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
       61 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      121 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      181 atcgataagc ttgatatcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      241 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      301 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      361 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      421 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      481 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      541 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
      601 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
      661 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
      721 agcttacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccgggcc accatgggac
      781 ccaagaaaaa acgcaaggtg ggaagcggat ccgccaaaaa caccattacc aaaacactga
      841 aactgcgtat tgtgcgtccg tataatagcg cagaagtgga aaaaattgtt gccgacgaaa
      901 aaaacaaccg cgaaaaaatc gcactggaaa agaacaaaga caaagtgaaa gaagcctgca
      961 gcaaacatct gaaagttgca gcatattgta ccacacaggt tgaacgtaat gcatgcctgt
     1021 tttgtaaagc acgtaaactg gatgacaaat tctaccaaaa actgcgtggt cagtttccgg
     1081 atgcagtttt ttggcaagaa atcagcgaaa tttttcgcca gctgcagaaa caggcagcag
     1141 aaatctataa tcagagcctg atcgaactgt actacgagat ttttatcaaa ggcaaaggta
     1201 ttgcaaatgc cagcagcgtt gaacattatc tgagtagagt ttgttataga cgtgcagcag
     1261 aactgtttaa aaacgcagca attgcaagcg gtctgcgtag caaaatcaaa agcaattttc
     1321 gtctgaaaga actgaaaaac atgaaaagtg gtctgccgac caccaaaagc gataattttc
     1381 cgattccgct ggttaaacag aaaggtggtc agtataccgg ttttgaaatt agcaatcata
     1441 atagcgactt catcatcaag attccgtttg gtcgttggca ggtcaaaaaa gagattgata
     1501 aatatcgtcc gtgggagaaa tttgactttg aacaggttca gaaaagcccg aaaccgatta
     1561 gcctgctgct gagcacccag cgtcgtaaac gtaataaagg ttggagcaaa gatgaaggca
     1621 ccgaagccga aatcaaaaaa gttatgaatg gcgattatca gaccagctac attgaagtta
     1681 aacgtggcag caaaatctgt gaaaaaagcg catggatgct gaatctgagc attgatgttc
     1741 cgaaaattga taaaggtgtg gatccgagca ttattggtgg tattgcagtt ggtgttagat
     1801 caccgctggt ttgcgcaatt aacaatgcat ttagccgtta tagcatcagc gataacgacc
     1861 tgtttcactt caacaagaaa atgtttgcac gtcgtcgtat cctgctgaaa aaaaaccgtc
     1921 ataaacgtgc aggtcatggt gcaaaaaaca aactgaaacc gatcaccatt ctgaccgaaa
     1981 aaagtgaacg ttttcgcaaa aagctgattg aacgttgggc atgtgaaatc gcggatttct
     2041 tcattaaaaa caaagttggc accgtgcaga tggaaaatct ggaaagcatg aaacgtaaag
     2101 aggacagcta ttttaacatt cgcctgcgtg gcttttggcc gtatgcagaa atgcagaaca
     2161 aaatcgaatt caaactgaag cagtatggca tcgaaattcg taaagttgca ccgaataata
     2221 ccagcaaaac ctgtagcaaa tgtggccatc tgaacaacta tttcaacttc gagtaccgca
     2281 agaaaaacaa attcccgcac tttaaatgcg aaaaatgcaa cttcaaagaa aacgccgcgt
     2341 ataatgcagc cctgaatatt tcaaacccga aactgaaaag caccaaagag agaccggcct
     2401 atccctatga cgtgcccgat tatgccagcc tgggcagcgg cgacggcatt ggtagtggga
     2461 gcaacggcag cagcctcgag ggccagagcg acgagagagc cctgctggac cagctgcaca
     2521 ccctgctgag caacaccgac gccaccggcc tggaggagat cgacagagcc ctgggcatcc
     2581 ccgagctggt gaaccagggc caggccctgg agcccaagca gggaggagga ggtagcggac
     2641 acgagaagtt ccccagcgac ctggacctgg acatgttcaa cggcagcctg gagtgcgaca
     2701 tggagagcat catcagaagc gagctgatgg acgccgacgg cctggacttc aacttcgaca
     2761 gcggaggagg aggtagcgga ttcgtgaccc tgaaggacgt gggcatggac ttcaccctgg
     2821 gcgactggga gcagctgggc ctggagcagg gcgacacctt ctgggacacc gccctggaca
     2881 actgccagga cctgttcctg ctggaccccc cccatatggg tggaggctcc ggggaagatc
     2941 ctgctgccaa acgcgttaaa ctagacatgg gaagcggagt gagcaagggc gaggaggata
     3001 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     3061 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3121 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     3181 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     3241 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     3301 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     3361 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     3421 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3481 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3541 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3601 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3661 gcggcatgga cgagctgtac aagtaagcgg ccgcataaga ctctagagtc gacctgcagg
     3721 catgcaagct tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
     3781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     4021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     4081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     4141 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     4201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     4261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     4321 tttgggccgc ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta
     4381 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4441 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     4501 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4561 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4621 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4681 agcatctaga attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac
     4741 ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg
     4801 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg
     4861 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca
     4921 gcgaaccaat cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg
     4981 gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     5041 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     5101 gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     5161 cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     5221 gagagcgtcg aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5281 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     5341 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5401 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5461 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5521 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5581 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5641 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5701 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5761 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5821 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5881 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5941 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6001 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6061 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6121 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6181 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6241 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6301 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6361 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6421 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6481 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6541 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6601 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6661 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6721 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6781 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6841 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6901 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6961 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7021 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7081 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7141 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7201 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7261 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7321 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7381 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7441 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
     7501 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7561 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7621 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7681 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7741 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7801 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     7861 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     7921 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     7981 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     8041 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     8101 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     8161 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     8221 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     8281 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     8341 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     8401 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     8461 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     8521 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     8581 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     8641 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     8701 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     8761 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     8821 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     8881 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     8941 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     9001 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     9061 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     9121 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     9181 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     9241 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     9301 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     9361 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     9421 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     9481 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     9541 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     9601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     9721 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     9781 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     9841 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     9901 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     9961 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
    10021 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
    10081 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
    10141 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10201 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
    10261 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
    10321 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
    10381 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    10441 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    10501 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    10561 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    10621 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    10681 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    10741 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    10801 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    10861 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    10921 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    10981 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
    11041 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
    11101 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
    11161 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
    11221 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
    11281 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    11341 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    11401 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    11461 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    11521 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    11581 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    11641 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    11701 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    11761 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    11821 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    11881 ttcgattagt gaacggatct cgacggtat
//
