LOCUS       sequence-408844-       10489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0684
FEATURES             Location/Qualifiers
     source          1..10489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..243
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             244..424
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    471..596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1089..1322
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1507..1551
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1782..1992
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1804..2000
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1851..1986
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2032..2634
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2032..2051)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2528..2548
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2932..4110
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3163..4101
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4058..4078
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5180..5197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    6358..6946
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6411..6431)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6829..6840)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7018..7251
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7329..7450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7366..7385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7420..7439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7490..7625
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7645..7664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7646..7664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7674..7691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7674..7690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7683..7705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7832..8287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7919..7938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8129..8150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8313..8417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8418..8486,8487..9278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8636..8655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9449..10037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9938..9957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10191..10208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10325..10346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10361..10366,10367..10384,10385..10391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10399..10415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10404..10426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10423..10439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10423..10439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10458..10478
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10460..10478
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
       61 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
      121 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
      181 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
      241 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      301 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      361 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      421 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      481 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      541 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      601 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
      661 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
      721 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
      781 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
      841 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
      901 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
      961 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1021 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1081 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1141 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1201 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1261 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1321 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1381 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1441 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1501 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1561 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1621 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     1681 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca
     1741 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     1801 gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     1981 gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag
     2041 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     2101 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     2161 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     2221 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     2281 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     2341 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     2401 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     2461 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     2521 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     2581 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt ttaaacagag
     2881 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     2941 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     3001 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     3061 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     3121 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     3181 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     3241 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     3301 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     3361 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     3421 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     3481 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     3541 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     3601 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     3661 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     3721 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     3781 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     3841 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     3901 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     3961 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     4021 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     4081 agtttttttc ttccatttca ggtgtcgtga ggctagcacc gccaccatgc acggagccgc
     4141 cagagcgcca gccaccagcg tgagtgccga ctgctgcatc ccggccggct tgcgcctcgg
     4201 accggtgcct ggtaccttca agctgggcaa gtacctgtca gaccgcaggg agcccgggcc
     4261 taagaaaaag gtgcgcatgg tgagagggga gctggtggac gagtcggggg gctcccctct
     4321 ggagtggata gggttaatcc gggcagccag aaactcccag gaacagactc tggaagctat
     4381 tgcagactta cccggaggac agatcttcta ccgagcattg cgagacgtcc agccagggga
     4441 ggagctgaca gtgtggtatt ctaactcctt ggctcagtgg ttcgacatcc ccaccacagc
     4501 gactccgact cacgacgaga aaggggagga gcgctacatc tgctggtact gctggaggac
     4561 gtttagatac cccaacagcc ttaaggcaca cctgcgtttc cactgcgtgt tcagcggcgg
     4621 tggaggcggc gccttcctgc accacgaaca cgcggctcgc caaggcgccg tcccagcggc
     4681 tgatggcctc ggtctctccc caaaaccccc ggcgcccgat ttcgccgcgc cttcccaggc
     4741 aggaactttg cgaccccacc ccctgggccc gccaccagtt caggcctgcg gtgcgcggga
     4801 gggcatcaag cgcgaggcct cttccgcgcc ctcggccacc tcgccgaccc caggcaagtg
     4861 ggggcagccc aagaagggca aggagcagct ggaccgtgcc ctggacatga gcggagccgc
     4921 ccgaggacaa gggcacttcc tcggcatcgt gggcggctcc tcggcggggg tcggcagcct
     4981 ggctttctac cccggcgtgc gctcagcttt caagcccgcc ggcctagcga gggcggcggc
     5041 ggccgctcac ggcgacccct accgggagga gagcagcagc aagcaaggag ccggcctcgc
     5101 tttgggcagg ctgctgggcg ggggccgggc gtgcgggcgc cccgggagcg gggagaactc
     5161 ggcggcgggc ggcgcgggtc accaccatca ccaccacgcg caccaccacc accatcccaa
     5221 gtgcctgctc gctggggacc cgccgccgcc gccgccgcct ggcctgccct gctctggggc
     5281 cctgcgcggc ttccctctgc tctccgtccc cccggaagag gcgtccgcct tcaagcacgt
     5341 ggagcgcgcc ccgcccgcag ccgccgcgct gccaggagcg cgttatgcgc agctgccccc
     5401 tgcgccgggg ttgcccctcg agcgctgcgc gctgccgccc ctcgacccgg gcggtctcaa
     5461 agcctatccg ggtggtgagt gcagccacct gcccgccgtc atgccggcct ttacagtcta
     5521 caacggggag ctgctctacg gctcaccggc caccaccgct tattacccgc tcaaattgca
     5581 cttcggcggg ctgctgaagt atccggagtc catctcctac ttcagcgggc ctgcagcggc
     5641 cgccctaagc cccgccgagc tggggtcgct ggccagcatc gaccgagaga tcgccatgca
     5701 caatcagcag ctgtccgaga tggctgccgg gaagggtcgc ggacgcctgg actcggggac
     5761 gttgccaccg gccgtcgcgg cggcgggagg caccgggggc ggcggcagcg gaggcagcgg
     5821 cgcaggtaag cccaagaccg gccacctgtg cctctactgt ggcaagctgt actcgcgcaa
     5881 gtatgggctc aagatccaca tgcggacgca cacgggctac aagccactca agtgcaaagt
     5941 ctgtctgcgg cccttcggcg accccagcaa tctcaacaag cacatccggc tgcacgccga
     6001 gggcaatacg ccctaccgct gcgagttctg cggcaaggta cttgtgcgcc gccgggacct
     6061 ggagcgacat gtcaagtccc gccaccctgg ccagagtctg ctcgccaaag cgggcgacgg
     6121 cccgggtgcc gagcccggct atcccccgga gcctggggat cccaagagcg acgacagtga
     6181 cgtggacgtc tgcttcacag acgaccagag cgaccccgag gttgggggcg gcggggagcg
     6241 cgacttgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     6301 atcttgtgga aaggacgatc cggcattagc taatattccc acacgcgtta agtcgacaat
     6361 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6421 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6481 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6541 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6601 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6721 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6781 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6841 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6901 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     6961 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7021 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     7261 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7321 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7381 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7441 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     7501 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7561 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7621 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     7681 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7741 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7801 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     7861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8281 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8641 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     8701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8881 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8941 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10081 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10141 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10201 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10261 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10321 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10381 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
    10441 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagct
//
