LOCUS       sequence-408850-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0814
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..257
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             258..438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    485..610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1103..1336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1521..1565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1796..2006
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1818..2014
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1865..2000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2046..2648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2046..2065)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2542..2562
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2691..2808
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2946..4124
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3177..4115
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4072..4092
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4135..4144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5469..6057
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5522..5542)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5940..5951)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6129..6362
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6440..6561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6477..6496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6531..6550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6601..6736
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6756..6775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6757..6775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6785..6802)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6785..6801)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6794..6816)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6943..7398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7030..7049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7240..7261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7424..7528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7529..7597,7598..8389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7747..7766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8560..9148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9049..9068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9302..9319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9436..9457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9472..9477,9478..9495,9496..9502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9510..9526
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9515..9537
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9534..9550
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9534..9550
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(9569..9586,1..3)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(9571..9586,1..3)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
       61 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      121 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      181 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      241 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      421 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      481 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      541 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      601 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      661 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      721 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      781 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      841 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      901 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
      961 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1021 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1081 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1141 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1201 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1261 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1321 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1381 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1441 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1501 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1561 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1621 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1681 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     1741 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1801 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1921 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1981 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
     2041 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     2101 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     2161 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     2221 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     2281 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     2341 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     2401 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     2461 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2521 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2581 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     2641 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
     2701 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2761 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2821 agggacagca gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa
     2881 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     2941 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     3001 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     3061 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3121 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3181 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3241 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     3301 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     3361 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     3421 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     3481 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     3541 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     3601 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     3661 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     3721 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     3781 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     3841 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3901 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     3961 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     4021 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     4081 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag caccgccacc
     4141 atggttcact gtgccggctg caaaaggccc atcctggacc gctttctctt gaacgtgctg
     4201 gacagggcct ggcacgtcaa gtgcgtccag tgctgtgaat gtaaatgcaa cctgaccgag
     4261 aagtgcttct ccagggaagg caaactctac tgcaagaacg acttcttccg gtgtttcggt
     4321 accaaatgcg caggctgcgc tcagggcatc tcccctagcg acctggtgcg gagagcgcgg
     4381 agcaaagtgt ttcacctgaa ctgcttcacc tgcatgatgt gtaacaagca gctctccact
     4441 ggcgaggaac tctacatcat cgacgagaat aagttcgtct gcaaagagga ttacctaagt
     4501 aacagcagtg ttgccaaaga gaacagcctt cactcggcca ccacgggcag tgaccccagt
     4561 ttgtctccgg attcccaaga cccgtcgcag gacgacgcca aggactcgga gagcgccaac
     4621 gtgtcggaca aggaagcggg tagcaacgag aatgacgacc agaacctggg cgccaagcgg
     4681 cggggaccgc gcaccaccat caaagccaag cagctggaga cgctgaaggc cgccttcgct
     4741 gctacaccca agcccacccg ccacatccgc gagcagctgg cgcaggagac cggcctcaac
     4801 atgcgcgtca ttcaggtctg gttccagaac cggcgctcca aggagcggag gatgaagcag
     4861 ctgagcgccc tgggcgcccg gcgccacgcc ttcttccgca gtccgcgccg gatgcggccg
     4921 ctggtggacc gcctggagcc gggcgagctc atccccaatg gtcccttctc cttctacgga
     4981 gattaccaga gcgagtacta cgggcccggg ggcaactacg acttcttccc gcaaggcccc
     5041 ccgtcctcgc aggcccagac accagtggac ctacccttcg tgccgtcatc tgggccgtcc
     5101 gggacgcccc tgggtggcct ggagcacccg ctgccgggcc accacccgtc gagcgaggcg
     5161 cagcggttta ccgacatcct ggcgcaccca cccggggact cgcccagccc cgagcccagc
     5221 ctgcccgggc ctctgcactc catgtcggcc gaggtcttcg gacccagccc gcccttctcg
     5281 tcgctgtcgg tcaacggtgg ggcgagctac ggaaaccacc tgtcccaccc ccccgaaatg
     5341 aacgaggcgg ccgtgtggta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     5401 tggctttata tatcttgtgg aaaggacgag tctctgcgaa gactggagta gctacgcgtt
     5461 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5521 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5581 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5641 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5701 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5761 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5821 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5881 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5941 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6001 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     6061 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6121 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     6181 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6241 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6301 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6361 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     6421 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6541 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     6601 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6661 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     6721 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     6781 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6841 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6901 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     6961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7381 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7441 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7501 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7561 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7621 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7681 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     7741 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     7801 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     7861 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     7921 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     7981 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8041 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8101 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8161 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8221 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8281 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8341 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8401 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8461 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8521 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8581 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8641 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8701 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8761 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9121 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9181 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9241 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9301 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9361 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     9421 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9481 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9541 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaa
//
