LOCUS       sequence-408852-        3965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP
            output.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ramakrishnan P, Tabor JJ
  TITLE     Repurposing Synechocystis PCC6803 UirS-UirR as a UV-violet/green 
            photoreversible transcriptional regulatory tool in E. coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 27.
  PUBMED    27120220
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 27."
COMMENT     Sequence Label: pPR219.05
FEATURES             Location/Qualifiers
     source          1..3965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     568..587
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     822..840
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        865..899
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     terminator      1736..1807
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1823..1850
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2112..2825
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2852..2923
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2939..2966
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3168..3756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3248..3267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3844..3938)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(3959..3965,1..653))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
       61 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      121 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      181 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
      241 aaccctttag ggaaataggc caggttttca ccataacacg ccacatcttg cgaatatatg
      301 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      361 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      421 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      481 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      541 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      601 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      661 tccttagctc ctgaaaatct cgataactca aaaaatacga ccggtagtga tcttatttca
      721 ttatggtgaa agttggaacc tcttacgggc cggccaacgt ctcattttcg ccagatatcg
      781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      841 cctttcgtct tcacctcgag ctgatttata gctagctcag cccttggtac aatgctagcc
      901 agtgggccca agttcactta aaaaggagat caacaatgaa agcaattttc gtactgaaac
      961 atcttaatca tgcgatggac ggtttctaat ggtaaccaaa atactgattg tagaggatga
     1021 aaggctagtg gcccaacaca ttgcccaatt attaaaaagt gatggctatg aaatttgtgt
     1081 gatcgccagt gatggagcaa cggcgctgaa aaaaattgct gagttttatc cagatctagt
     1141 tttactagat attcgtatta aaggagagat cgacgggata gaggtggcgg aacggataaa
     1201 atctctttac tccatcccca ttgtttatct cacagctttt tctgatgggg aaaccctgga
     1261 gcgggcccag aaaaccaatc cccagggcta tgtgattaag ccttttcggc gggaacaact
     1321 attatccacc gtggcgatcg ccatagccaa tcatcaacag caacgaaaac ctgaggaaga
     1381 taccctctcc acgtcaacgg gccattatcg cctgcaaccg actttggact atatcgaaga
     1441 gcatctcgac caaggaatta ccgttgaatt tctggccggg gcgatcggca tgagcacagc
     1501 ctacttttgt cgctttttcc aaaaggaaat gggatgttcc ccctatcaat ttattatcca
     1561 acaacgggtt gagcgggcca aagccatact actggaaaga gaattgtcca ttagcgaagt
     1621 agctttgaga tgcggcttca gttcccacag tcaacttaac caccattttc gtaatctctt
     1681 gggcattacc ccgaaagaat atcgtagtcg ctagtaataa tctagaccag ggcatcaaat
     1741 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1801 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatagtaa
     1861 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
     1921 cgacatcata acggttctgg caaatattct gaaatgagct ggttagctag tcacagtttc
     1981 caagcaagat ccgggcgaaa aaataaaatc gatgtcaaaa atcaactagt ttgtcaaaaa
     2041 tcgaccagga cagggttggg aatttttcct aggatgggac tgtcagaagt ctccccttaa
     2101 ggataagaat aatgcgtaaa ggcgaagagc tgttcactgg tgtcgtccct attctggtgg
     2161 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
     2221 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggtacctt
     2281 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
     2341 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
     2401 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
     2461 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaagac ggcaatatcc
     2521 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
     2581 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
     2641 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
     2701 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     2761 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     2821 acaaatgatg ataataatct agaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2881 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2941 ctcaccttcg ggtgggcctt tctgcgttta taggtacccg tggatcctct agagtcgacc
     3001 tgcaggcatg caagcttcct aggcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3061 gcggtaatac ggttatccac agaatcaggg gataacgcat gcaagaacat gtgagcaaaa
     3121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3601 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat tactagtgct
     3841 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     3901 gagttctgag gtcattactg gatctatcaa cagtagtcca agcgagctcg atatcaaatt
     3961 acgcc
//
