LOCUS       sequence-408857-       10704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1813
FEATURES             Location/Qualifiers
     source          1..10704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5987..5998
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6561..7149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6614..6634)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7032..7043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7221..7454
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7532..7653
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7569..7588)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7623..7642
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7693..7828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7848..7867)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7849..7867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7877..7894)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7877..7893)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7886..7908)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8035..8490
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8122..8141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8332..8353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8516..8620
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8621..8689,8690..9481)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8839..8858)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9652..10240
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10141..10160
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10394..10411
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10528..10549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10564..10569,10570..10587,10588..10594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10602..10618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10607..10629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10626..10642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10626..10642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10661..10681
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10663..10681
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgctg ccatgtcatt caccatcacg
     4141 ctctgatcag gtgaatctgg gaccctccat caactccaag ctgctgggga tgtccaccca
     4201 gaactatgcc ctgatgcagg ttgctggcca ggaggggaca ttttctcttg ttgctctgcc
     4261 acatgttgcc tcagctcagc caattcagaa acccagaatg tccctacctg aaaacctgaa
     4321 acttcctatt cctagatatc aaccccctag aaatagcaaa gcatcaagaa agaaacccat
     4381 cctgatcttt cctaagagtg gctgtagcaa agctcctgcc caaacccaaa tgtgtcctca
     4441 gatgtcccct tccccacctc accaccctga actcctgtac aaacccagtc catttgagga
     4501 agtaccatca ctagagcaag ccccagccag cattagcaca gctgcgctga ccaatggaag
     4561 tgaccatggg gacttgagac caccagtgac caacacccat ggcagtctga accctcctgc
     4621 taccccagca tcatccacac cagaggagcc tgccaagcag gacctcacag ctctttcagg
     4681 gaaagcacac tttgtaagca agataacatc tagtaaacct tctgctgttg ccagtgaaaa
     4741 atttaaagaa caagttgatc ttgcaaaaac catgaccaat ttatcaccaa ccattcttgg
     4801 caatgcagtt cagttgatct cttcagtccc caaagggaaa ctgccaatcc caccctactc
     4861 aagaatgaag acaatggagg tttacaaaat caaatcagat gctaacattg caggtttttc
     4921 tttaccagga cctaaggccg actgtgataa gataccctcc accacagaag gctttaatgc
     4981 agccaccaag gtggcaagca ggctacctgt tccacaagtg tcacagcaga gtgcctgtga
     5041 aagtgccttt tgtccaccca ccaaacttga tcttaaccac aaaacaaaac tgaacagtgg
     5101 agcagcaaag agaaaaggaa gaaaacggaa ggtaccagat gaaattttgg catttcaggg
     5161 aaaaaggagg aaatatatca ttaataagtg tagagatggt aaagaaagag taaaaaatga
     5221 tccccaagaa ttcagagacc aaaagctggg gaccctgaaa aaataccgta gcattatgcc
     5281 caaacctatc atggtcatac ccactttggc ctccctggct tctccaacta cactacagtc
     5341 ccagatgctt gggggcctag gacaggatgt tttgttaaat aattcactca ctcctaaata
     5401 tcttggctgt aagcaagaca acagctcttc ccctaagccc agctccgtgt tcagaaatgg
     5461 attctctggc attaagaagc cttggcacag atgtcacgtc tgcaaccacc acttccagtt
     5521 caaacagcac cttcgagacc acatgaatac acacaccaac agacgccctt acagttgtcg
     5581 gatttgtcgc aagtcctatg tacgtcctgg cagcctgagc acacacatga aacttcatca
     5641 tggtgagaac cgtctgaaga aactcatgtg ttgtgagttt tgtgcaaaag tgtttggcca
     5701 catccgagtc tattttggcc atctgaaaga agtgcatagg gttgtgatca gcactgagcc
     5761 tgcgcccagt gaactgcagc caggagacat accaaagaac agagacatga gtgtgcgagg
     5821 catggaggga tcattggaga gggaaaacaa gtccaacctg gaagaagact tccttctaaa
     5881 ccaggcagac gaagtcaaat tacaaatcaa atgtggtcgt tgtcagatta ctgctcagtc
     5941 ttttgcggaa ataaaatttc atttacttga tgttcatgga gaggaaattg agggcaggct
     6001 acaagaaggg accttcccag gaagcaaggg gactcaggaa gagttggtgc agcacgctag
     6061 ccccgactgg aaaaggcatc ctgagagagg gaagccggag aaggttcatt cctcctccga
     6121 ggaatcacat gcatgtccaa gactgaaaag gcagctccac cttcatcaga atggcgtgga
     6181 aatgctcatg gaaaatgaag gaccccagtc aggaaccaac aagccaaggg aaacctgcca
     6241 gggccctgag tgtcctggcc tccacacgtt tctcttgtgg tcccattcag gctttaactg
     6301 cctgctttgt gcagagatgc tgggacggaa agaggacctc ctccaccact ggaagcacca
     6361 gcataactgt gaggaccctt ccaaactgtg ggctatttta aatacggtct ccaaccaggg
     6421 agtgatcgaa ctttccagtg aagctgagaa atagtaatga actagtccgt aacttgaaag
     6481 tatttcgatt tcttggcttt atatatcttg tggaaaggac gatctcctga acagtcctat
     6541 cccaccacgc gtaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6601 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6661 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6721 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6781 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6841 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6901 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6961 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7021 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7081 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7141 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7201 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     7261 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7321 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7381 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7441 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     7501 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7681 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     7741 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     7801 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7861 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7921 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7981 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     8281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8341 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8401 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8461 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     8521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8581 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8641 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8701 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8761 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8821 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8881 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8941 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9001 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9061 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9121 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9181 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9241 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9301 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9361 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9421 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9481 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9541 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9601 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9661 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9721 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9781 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9841 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9901 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9961 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10021 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10081 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10201 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10261 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10321 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    10381 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
    10441 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    10501 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
    10561 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
    10621 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
    10681 ggaacaaaag ctggagctgc aagc
//
