LOCUS       sequence-408866-       11821 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of HLA-G SCT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-HLA-G SCT-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(292..311)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        483..862
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        864..1062
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1080..1260
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1307..1432
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1925..2158
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2343..2387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2685..2802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2891..2909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2926..2944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2962..2980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2998..3016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3099..3137
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3134..3158
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3233..4444
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4281..4300
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5921..5974
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5978..5980,5981..6694)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6020..6041)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6281..6300)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6628..6649
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6754..7342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6807..6827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7225..7236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7345..7361)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7346..7362)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7871..8051
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8077..8094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8083..8307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8353..8781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8440..8459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8650..8671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8790..8810)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8795..9124
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8975..9110
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9037..9056
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9172..9219
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9238..9612
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9742..9863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9779..9798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9833..9852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9912..9928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9912..9928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9925..9947)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9936..9952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9960..9966,9967..9984,9985..9990))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10005..10026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10143..10160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10314..10902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10394..10413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11073..11821,1..43,44..112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11696..11715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
      301 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
      361 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
      421 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      481 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      541 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      601 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      661 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      721 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      781 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      841 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      901 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      961 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1021 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg
     1081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1261 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     1321 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     1381 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     1441 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1501 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1561 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1621 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1681 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1741 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     1801 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1861 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1921 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1981 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     2041 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     2101 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     2161 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     2221 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     2281 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     2341 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     2401 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     2461 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     2521 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     2581 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     2641 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2701 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2761 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2821 agcagagatc cagtttggtt aattctagtt ttactcccta tcagtgatag agaacgtatg
     2881 tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata
     2941 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     3001 ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc
     3061 gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg
     3121 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt cgttaacgga
     3181 tcctctagag ctcagcctcg agatttaaat ttaattaacc cacccaagat ctggcctccg
     3241 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     3301 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     3361 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     3421 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     3481 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     3541 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     3601 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     3661 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     3721 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     3781 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     3841 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     3901 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     3961 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     4021 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     4081 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     4141 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     4201 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     4261 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     4321 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     4381 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     4441 agacgaagct tggtaccgag ctcggatctc caccccgtac cggttaacca tgtccagatc
     4501 cgtggccctg gctgtgctgg ctctgctgtc cctgagcggc ctcgaggcca aaggaccccc
     4561 cgctgctctg acactgggct gtggcgcttc cggaggcggc ggcagcggag gcggaggcag
     4621 cattcagagg acccctaaga tccaggtgta ctccaggcat cctgccgaga acggcaaatc
     4681 caatttcctc aattgctacg tgtccggctt ccatccctcc gacatcgaag tggacctcct
     4741 caagaacgga gagagaatcg aaaaggtgga gcactccgac ctgtccttct ccaaagactg
     4801 gtccttctac ctgctgtatt acacagagtt tacccctacc gagaaggacg agtacgcctg
     4861 tagagtgaat cacgtgacac tgtcccagcc caagattgtg aaatgggaca gggacatggg
     4921 cggcggagga agcggaggcg gcggaagcgg cggcggcggc agcggcggcg gcggctccgg
     4981 ctcccacagc atgaggtact tttccgccgc cgtgtccagg cccggcaggg gcgaacctag
     5041 atttatcgct atgggctacg tcgacgacac ccagtttgtg aggttcgaca gcgactccgc
     5101 ctgccccaga atggaaccta gagccccttg ggtcgagcag gaaggccccg agtactggga
     5161 ggaggagacc aggaatacca aggcccacgc tcagaccgac aggatgaatc tgcagaccct
     5221 gaggggatgc tacaaccaaa gcgaggccag ctcccacacc ctgcagtgga tgatcggctg
     5281 tgatctggga tccgacggca ggctcctgag aggctatgag cagtacgcct acgatggcaa
     5341 ggattacctg gctctcaacg aggacctcag atcctggaca gccgccgata cagccgctca
     5401 gatctccaag aggaagtgcg aggctgccaa tgtcgctgag cagaggaggg cctacctgga
     5461 aggaacctgc gtcgaatggc tgcacaggta cctggaaaac ggcaaggaaa tgctccagag
     5521 ggccgaccct cctaagacac acgtcacaca ccaccccgtg ttcgattatg aggccacact
     5581 caggtgctgg gctctcggct tttatcccgc cgagattatc ctgacctggc agagagatgg
     5641 cgaggatcag acccaggacg tcgagctggt ggagacaagg cctgccggcg acggaacatt
     5701 tcagaaatgg gctgctgtgg tggtgccctc cggcgaggaa cagaggtaca catgccacgt
     5761 gcagcatgaa ggactccccg agcccctgat gctgagatgg aagcagtcct ccctgcctac
     5821 aatccccatc atgggcatcg tggctggcct cgtcgtcctc gctgctgtgg tgaccggcgc
     5881 tgccgtcgcc gccgtgctgt ggagaaagaa gtcctccgat gagggcagag gaagtcttct
     5941 aacatgcggt gacgtggagg agaatcccgg ccctgtggtg agcaagggcg aggagctgtt
     6001 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     6061 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     6121 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttga cctacggcgt
     6181 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     6241 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     6301 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     6361 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     6421 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagacccg
     6481 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     6541 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     6601 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     6661 gatcactctc ggcatggacg agctgtacaa gtaatgtaca agtaaagcgg ccgcgactct
     6721 agtccctccc aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     6781 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6841 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6901 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6961 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     7021 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     7081 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     7141 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     7201 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     7261 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     7321 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca
     7381 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7441 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7561 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7621 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg
     7681 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga
     7741 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7801 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc
     7861 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7921 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7981 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8041 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8281 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8341 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8401 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8461 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8521 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8581 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8641 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8701 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8761 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8941 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9061 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9121 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     9181 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     9241 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag
     9301 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg
     9361 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac
     9421 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc
     9481 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg
     9541 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag
     9601 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9721 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9841 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9961 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    10381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    10441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    10501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    10561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    10621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    10681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    10741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    11341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    11401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    11461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    11521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    11581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    11641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    11701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    11761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11821 a
//
