LOCUS       sequence-408880-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the ETF1 transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0343
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..242
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             243..423
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    470..595
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1088..1321
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1506..1550
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1781..1991
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1803..1999
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1850..1985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2031..2633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2031..2050)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2527..2547
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2676..2793
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2931..4109
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3162..4100
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4057..4077
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4120..4129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5547..6135
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5600..5620)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6018..6029)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6207..6440
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6518..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6555..6574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6609..6628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6679..6814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6834..6853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6835..6853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6863..6880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6863..6879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6872..6894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7021..7476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7108..7127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7318..7339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7502..7606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7607..7675,7676..8467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7825..7844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8638..9226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9127..9146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9380..9397
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9514..9535
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9550..9555,9556..9573,9574..9580)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9588..9604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9593..9615
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9612..9628
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9612..9628
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9647..9667
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9649..9667
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
       61 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      121 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      181 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      241 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      301 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      361 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      421 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      481 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      541 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      601 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      661 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
      721 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
      781 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
      841 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
      901 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
      961 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1021 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1081 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1141 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1201 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1261 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1321 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1381 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     1441 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     1501 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     1561 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1621 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1681 caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa
     1741 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1801 catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1861 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1921 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     1981 aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt
     2041 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     2101 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     2161 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     2221 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
     2281 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     2341 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     2401 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     2461 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     2521 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     2581 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc
     2641 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2701 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2761 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2821 ccactttggc gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga
     2881 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     2941 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     3001 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     3061 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     3121 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     3181 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     3241 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     3301 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     3361 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     3421 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     3481 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     3541 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     3601 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     3661 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3721 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     3781 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     3841 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     3901 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3961 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     4021 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     4081 gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc ggacgacccc
     4141 agtgctgccg acaggaacgt ggagatctgg aagatcaaga agctcattaa gagcttggag
     4201 gcggcccgcg gcaatggcac cagcatgata tcattgatca ttcctcccaa agaccagatt
     4261 tcacgagtgg caaaaatgtt agcggatgag tttggaactg catctaacat taagtcacga
     4321 gtaaaccgcc tttcagtcct gggagccatt acatctgtac aacaaagact caaactttat
     4381 aacaaagtac ctccaaatgg tctggttgta tactgtggaa caattgtaac agaagaagga
     4441 aaggaaaaga aagtcaacat tgactttgaa cctttcaaac caattaatac gtcattgtat
     4501 ttgtgtgaca acaaattcca tacagaggct cttacagcac tactttcaga tgatagcaag
     4561 tttggattca ttgtaataga tggtagtggt gcactttttg gcacactcca aggaaacaca
     4621 agagaagtcc tgcacaaatt cactgtggat ctcccaaaga aacacggtag aggaggtcag
     4681 tcagccttgc gttttgcccg tttaagaatg gaaaagcgac ataactatgt tcggaaagta
     4741 gcagagactg ctgtgcagct gtttatttct ggggacaaag tgaatgtggc tggtctagtt
     4801 ttagctggat ccgctgactt taaaactgaa ctaagtcaat ctgatatgtt tgatcagagg
     4861 ttacaatcaa aagttttaaa attagttgat atatcctatg gtggtgaaaa tggattcaac
     4921 caagctattg agttatctac tgaagtcctc tccaacgtga aattcattca agagaagaaa
     4981 ttaataggac gatactttga tgaaatcagc caggacacgg gcaagtactg ttttggcgtt
     5041 gaagatacac taaaggcttt ggaaatggga gctgtagaaa ttctaatagt ctatgaaaat
     5101 ctggatataa tgagatatgt tcttcattgc caaggcacag aagaggagaa aattctctat
     5161 ctaactccag agcaagaaaa ggataaatct catttcacag acaaagagac cggacaggaa
     5221 catgagctta tcgagagcat gcccctgttg gaatggtttg ctaacaacta taaaaaattt
     5281 ggagctacgt tggaaattgt cacagataaa tcacaagaag ggtctcagtt tgtgaaagga
     5341 tttggtggaa ttggaggtat cttgcggtac cgagtagatt tccagggaat ggaataccaa
     5401 ggaggagacg atgaattttt tgaccttgat gactactagt aatgaactag tccgtaactt
     5461 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacta tcgtaaatca
     5521 ggattctgca cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5581 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5641 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5701 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5761 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5821 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5881 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5941 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6001 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6061 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6121 gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag
     6181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6241 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6421 agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt
     6481 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6781 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6841 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6901 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6961 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     7021 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7081 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7141 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7201 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7261 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7321 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7381 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7441 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     7501 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7561 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7621 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7681 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7741 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7801 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7861 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7921 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7981 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8041 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8101 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8161 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8221 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8281 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8341 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8401 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8461 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8521 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8581 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8761 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9301 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9361 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9421 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9481 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9541 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9601 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     9661 ctaaagggaa caaaagctg
//
