LOCUS       sequence-408883-        9118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0699
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..257
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             258..438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    485..610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1103..1336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1521..1565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1796..2006
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1818..2014
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1865..2000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2046..2648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2046..2065)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2542..2562
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2691..2808
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2946..4124
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3177..4115
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4072..4092
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4135..4144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5001..5589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5054..5074)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5472..5483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5661..5894
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5972..6093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6009..6028)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6063..6082
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6133..6268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6288..6307)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6289..6307)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6317..6334)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6317..6333)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6326..6348)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6475..6930
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6562..6581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6772..6793
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6956..7060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7061..7129,7130..7921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7279..7298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8092..8680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8581..8600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8834..8851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8968..8989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9004..9009,9010..9027,9028..9034)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9042..9058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9047..9069
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9066..9082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9066..9082
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(9101..9118,1..3)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(9103..9118,1..3)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
       61 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      121 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      181 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      241 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      421 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      481 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      541 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      601 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      661 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      721 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      781 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      841 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      901 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
      961 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1021 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1081 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1141 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1201 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1261 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1321 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1381 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1441 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1501 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1561 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1621 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1681 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     1741 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1801 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1921 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1981 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
     2041 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     2101 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     2161 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     2221 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     2281 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     2341 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     2401 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     2461 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2521 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2581 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     2641 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
     2701 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2761 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2821 agggacagca gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa
     2881 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     2941 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     3001 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     3061 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3121 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3181 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3241 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     3301 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     3361 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     3421 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     3481 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     3541 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     3601 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     3661 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     3721 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     3781 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     3841 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3901 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     3961 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     4021 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     4081 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag caccgccacc
     4141 atggctcctg tgggcaagag aataggtggt ttgggggaca cacgggttgg aggcccgtgc
     4201 atatcccagc agcacgagtc tctggaaaaa gacaacctcg ccctgcggaa ggagatccag
     4261 tccctgcagg ccgagctggc gtggtggagc cggaccctgc acgtgcatga gcgcctgtgc
     4321 cccatggatt gtgcctcctg ctcagctcca gggctcctgg gctgctggga ccaggctgag
     4381 gggctcctgg gccctggccc acagggacaa catggctgcc gggagcagct ggagctgttc
     4441 cagaccccgg gttcctgtta cccagctcag ccgctctctc caggtccaca gcctcatgat
     4501 tctcccagcc tcctccagtg ccccctgccc tcactgtccc ttggccccgc tgtggttgct
     4561 gaacctcctg tccagctgtc ccccagccct ctcctgtttg cctcgcacac tggttccagc
     4621 ctgcaggggt cttcctctaa gctcagtgcc ctccagccca gcctcacggc ccaaactgcc
     4681 cctccacagc ccctcgagct ggagcatccc accagaggga agctggggtc ctctcccgac
     4741 aacccttcct ctgccctggg gcttgcacgt ctgcagagca gggagcacaa acctgctctc
     4801 tcagcagcca cttggcaagg gctggttgtg gatcccagcc ctcaccctct cctggccttt
     4861 cctctgctct cctctgctca agtccacttc tagtaatgaa ctagtccgta acttgaaagt
     4921 atttcgattt cttggcttta tatatcttgt ggaaaggacg aggatgagcg acacttctaa
     4981 cagccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5401 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5581 cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5641 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5701 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5761 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5821 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5881 gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt atttataact
     5941 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     6001 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6061 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     6121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6181 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6241 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     6301 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     6361 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     6421 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     6481 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6541 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6601 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6661 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6721 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6781 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6841 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6901 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     6961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8941 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9001 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9061 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaa
//
