LOCUS       sequence-408884-       10963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10963)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0710
FEATURES             Location/Qualifiers
     source          1..10963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..244
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             245..425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    472..597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1090..1323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1508..1552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1783..1993
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1805..2001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1852..1987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2033..2635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2033..2052)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2529..2549
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2678..2795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2933..4111
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3164..4102
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4059..4079
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6833..7421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6886..6906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7304..7315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7493..7726
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7804..7925
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7841..7860)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7895..7914
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7965..8100
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8120..8139)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8121..8139)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8149..8166)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8149..8165)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8158..8180)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8307..8762
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8394..8413)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8604..8625
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8788..8892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8893..8961,8962..9753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9111..9130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9924..10512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10413..10432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10666..10683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10800..10821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10836..10841,10842..10859,10860..10866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10874..10890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10879..10901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10898..10914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10898..10914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10933..10953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10935..10953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
       61 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      121 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      181 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      241 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      301 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      361 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      421 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      481 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      541 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      601 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      661 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      721 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      781 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      841 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      901 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
      961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     1741 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     1801 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     1861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1921 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     1981 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     2041 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     2101 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     2161 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     2221 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     2281 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     2341 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     2401 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     2461 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     2521 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     2581 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     2641 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2701 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2761 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2821 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
     2881 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     2941 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     3001 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     3061 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     3121 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     3181 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     3241 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     3301 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     3361 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     3421 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     3481 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     3541 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     3601 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     3661 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     3721 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     3781 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     3841 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     3901 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     3961 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     4021 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     4081 aagttttttt cttccatttc aggtgtcgtg aggctagcac cgccaccatg tctttgcaag
     4141 actccactct ttccagagag gggaaaccag agggagagat tatggctgct gtgtttttct
     4201 cagttggacg cctgagccct gaagttaccc agccagatga agatcttcac cttcaggcag
     4261 aagaaacaca attggtaaag gaatcggtga catttaagga tgtggctata gacttcacat
     4321 tggaggagtg gaggttgatg gaccctacac agaggaacct gcacaaggat gtgatgctag
     4381 agaattacag gaatctggtc tccctggggc ttgcagtttc caaaccagac atgatatctc
     4441 atttggagaa tgggaaagga ccatgggtga cggtgagaga aatttcaaga attccctatc
     4501 ctgacatgga gcccaaacct gcaaccaaga aggctacacg aacaaaggct atttctgaag
     4561 atttatcaca ggaggccata ctagagaaac ttacagagaa tggtctgtgg gattccagaa
     4621 tggaagggtt atggaaatgg aatgatagga tattgagatt acaaaataat caggagaatc
     4681 atttgagtca aagaataatt ccactcaaga agactcccac tagtcaaaga ggctttagat
     4741 ttgaatctat tcttattcca gaaccaggca ttgccacaga agagcttcac agcagatgcc
     4801 aaacacaaga ggaaaatttc acagagaatt taaatttgat tacagatacc catttgggga
     4861 agataatttg caaggagatg aaaggcagca aagccataag gcagacttca gaactaactt
     4921 tggggaaaaa atccaataat aaggaaaaac cctacaaatg tagtacatgc gaaaaggcct
     4981 ttcattatag atcattgctc attcaacatc aaagaactca tactaaagaa aaaccttatg
     5041 aatgtaatga atgtgggaaa acattcagcc agccttcata tctcagtcag cacaaaaaaa
     5101 tccacactgg agaaaaaccc tataaatgta atgaatgtgg aaaggccttc attgcttctt
     5161 catcacttat ggtacatcag agaattcaca ctaaagagaa accttatcag tgtaatgtgt
     5221 gtgggaaatc ttttagccag tgtgcccgtc ttaatcagca ccagagaatt caaactggag
     5281 agaaacccta taaatgtagt gaatgcggga aagcttttag tgataaatca aaacttgcaa
     5341 gacatcagga aactcacaat ggtgagaaac cctacaaatg tgatgattgt gggaaagcct
     5401 ttaggaacaa gtcatatctt agtgtacatc agaagaccca cactgaagag aaaccatatc
     5461 agtgcaacga gtgtgggaag tcttttaaga ataccacaat ttttaatgtg catcagagga
     5521 ttcatactgg agagaaacct tttagatgta acgaatgtgg aaaagcctat agaagtaatt
     5581 caagccttat cgtacatata agaactcaca ctggggaaaa accctatgaa tgtaatgaat
     5641 gtgggaaagc attcaaccgc atcgcaaatt tcacagaaca tcagcgaatt cacacaggag
     5701 aaaaacccta taaatgtaat gaatgtggga aagcattcat taattattca tgccttactg
     5761 tacaccacag aatgcataca ggagagaaac cttataaatg tactgaatgt ggaaaggcct
     5821 tcatgcgttc ttcttctcta attatacatc agcgtattca tactgaagag aaaccttatc
     5881 tgtgcaatga atgtggggag tctttcagaa taaaatcaca cttaactgta catcagagaa
     5941 ttcacactgg agagaaacca tataaatgta ctgactgcga gagggcattc accaaaatgg
     6001 taaatctcaa ggagcatcag aaaattcata ctggagtgaa accctataaa tgttatgact
     6061 gtggaaagtc ctttaggact aaatcatacc ttattgtaca tcagaggacc catactggag
     6121 aaaaaccata taaatgtaat gaatgtgaga aagccttcac taatacatca cagcttaccg
     6181 tgcaccaacg aaggcatact ggagagaagc cctataaatg caatgaatgt ggaaaggttt
     6241 tcacaagtaa ctcaggcttt aatacacatc aaagaacaca tactggagag aaaccattta
     6301 aatgtaatga ctgtgggaaa gcatttagcc agatggtaca tgtcacagaa catcagaaaa
     6361 tccatagtgg agagaagccc tataagtgtg atgtctgtgg aaaagccttc aggaggggtt
     6421 cttaccttac agtgcattgg agaacacaca ctggagaaaa accctacacc tgtaaggaat
     6481 gtggaaaggg ttgtattact ctatcacagc taaccctaca tcagagaatt catactgggg
     6541 agaggcccta taaatgtgaa gaatgtggaa aagccttcag aactaactca gactttactg
     6601 tacacttgag gatgcatact ggagaaaaac cctataaatg taatgaatgt ggaaaagcct
     6661 tcaggagtag ttcgagcctt actgtacatc aaagaataca tcaaagagaa actcagttaa
     6721 tatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     6781 gtggaaagga cgatacactg tagccgcata gatgcgaacg cgttaagtcg acaatcaacc
     6841 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6901 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6961 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7021 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7081 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7141 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7201 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7261 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7321 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7381 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
     7441 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     7501 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     7561 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7621 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7681 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt
     7741 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7801 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7921 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     7981 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8041 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8101 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8161 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8221 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8281 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8341 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8401 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8461 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8521 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8581 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8641 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8701 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     8761 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8821 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8881 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8941 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9001 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9061 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     9301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    10561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    10621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    10681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    10741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    10801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    10861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
    10921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agc
//
