LOCUS       sequence-408887-       10975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0709
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..26
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        54..280
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             281..461
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    508..633
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1126..1359
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1544..1588
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1819..2029
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1841..2037
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1888..2023
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2069..2671
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2069..2088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2565..2585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2714..2831
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2969..4147
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3200..4138
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4095..4115
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4158..4167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6881..7469
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6934..6954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7352..7363)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7541..7774
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7852..7973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7889..7908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7943..7962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8013..8148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8168..8187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8169..8187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8197..8214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8197..8213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8206..8228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8355..8810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8442..8461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8652..8673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8836..8940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8941..9009,9010..9801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9159..9178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9972..10560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10461..10480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10714..10731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10848..10869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10884..10889,10890..10907,10908..10914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10922..10938
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10927..10949
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10946..10962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10946..10962
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
       61 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      121 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      181 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      241 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      301 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      361 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      421 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      481 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      541 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      601 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      661 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      721 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
      781 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
      841 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
      901 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
      961 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1021 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1081 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1141 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1201 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1261 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1321 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1381 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1441 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1501 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1561 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1621 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1681 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1741 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     1801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     1861 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     1921 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     1981 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     2041 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     2101 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2161 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2221 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2281 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2341 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2401 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2461 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2521 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2581 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     2641 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
     2701 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2761 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2821 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     2881 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     2941 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     3001 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     3061 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     3121 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     3181 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     3241 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     3301 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     3361 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     3421 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     3481 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     3541 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     3601 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     3661 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     3721 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     3781 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     3841 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     3901 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     3961 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     4021 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     4081 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     4141 gtcgtgaggc tagcaccgcc accatggagc ccgagactgc gctgtggggc ccggatctgc
     4201 agggtccgga acagagcccc aacgatgctc acagaggtgc cgagagtgaa aacgaagagg
     4261 agagccctcg gcaggaaagt tctggggagg agatcatcat gggagacccg gctcagagtc
     4321 cagaatccaa ggactcaaca gagatgtccc tggagagatc ctcccaggac ccctctgtcc
     4381 cccagaaccc cccaacccca ctgggtcact ccaatccctt ggaccaccag atccccctgg
     4441 accccccagc cccggaggta gtccctaccc catctgactg gaccaaggcc tgcgaggcca
     4501 gctggcagtg gggcgctctc accacatgga acagcccccc agtcgtcccc gccaacgagc
     4561 ccagcctgcg ggagctggtg cagggccgcc cggcgggggc ggagaagccc tacatctgca
     4621 acgagtgcgg caagagcttc agccagtggt ccaagctgct gcggcaccag cgcatccaca
     4681 cgggagagcg gcccaacacc tgctccgagt gcggcaagag cttcacgcag agctcgcacc
     4741 tggtgcagca ccagcgcacg cacaccggcg agaagcccta caagtgcccc gactgcggca
     4801 agtgcttcag ctggagctcc aacctggtgc agcaccagcg cacgcacacg ggagagaagc
     4861 cctacaagtg cacggagtgc gagaaagcct tcacccagag caccaacctc atcaagcacc
     4921 agcgatccca caccggcgag aagccctaca agtgcggcga gtgccgccgg gctttctacc
     4981 gcagctcgga cctcatccag caccaggcca cccacacagg cgagaaaccc tacaagtgcc
     5041 ccgagtgcgg gaagcgcttc ggccagaacc acaacctcct caagcaccag aagatccacg
     5101 cgggcgagaa gccataccgc tgcaccgagt gcgggaagag cttcatccag agctcggagc
     5161 tgacccagca ccagcgcacg cacacaggcg agaagcccta cgagtgccta gagtgcggca
     5221 agagcttcgg ccacagctcc accctcatca agcaccagcg gactcacctg cgcgaggacc
     5281 cgttcaagtg cccagtgtgc ggcaagacct tcaccctgag cgccacgttg ctgcggcacc
     5341 agcgcacgca cacgggcgag cggccctaca agtgcccaga gtgcggcaag agcttcagcg
     5401 tcagctccaa cctcatcaac caccagcgca tccaccgcgg cgagcggccc tacatctgcg
     5461 ccgactgcgg caagagcttc atcatgagct ccacccttat ccgccaccag cgcatccaca
     5521 ccggtgagaa gccctacaag tgttccgact gcggcaagag cttcatccgc agctcccacc
     5581 ttatccagca ccgccgcacg cacaccggcg agaagcccta caagtgcccc gagtgcggca
     5641 agagcttcag ccagagctcc aaccttatta cccacgtccg cacgcacatg gacgagaacc
     5701 tgttcgtgtg ctccgactgc gggaaggcct tcctggaagc ccacgagctg gagcagcacc
     5761 gggtgatcca tgagaggggg aagaccccag cgcgtagggc ccagggcgac agcctgctgg
     5821 ggctcgggga cccctccctg ctgaccccgc cgccgggagc caagccgcac aagtgtctcg
     5881 tgtgcggaaa gggcttcaac gacgagggca tcttcatgca acatcagagg atccacatcg
     5941 gagaaaaccc ctacaaaaat gcagacggcc tcatcgcaca cgcagccccc aaacctcctc
     6001 agttacgatc cccaaggctc cctttcagag ggaattccta ccccggggct gcggagggca
     6061 gagcggaggc ccccggacag ccccttaagc cgccggaggg tcaggagggc ttcagccaga
     6121 ggcgggggct gctgtcctcc aagacctaca tctgctccca ctgcggagag agcttcctgg
     6181 atcgctctgt gctcctccag catcagctca cccacggcaa cgaaaagccc tttctctttc
     6241 ctgattatag aattggccta ggggaaggcg cagggcccag ccccttctta agtgggaagc
     6301 cctttaaatg ccctgaatgc aaacaaagct ttggcctcag ctctgagctg ctgctgcacc
     6361 agaaagtcca tgcaggcggg aagagctccc agaagagtcc agagctgggg aagagctctt
     6421 ccgtcctcct ggagcatctc aggagccccc tgggggccag accctaccgc tgctcagatt
     6481 gcagggcctc cttcctcgac cgcgtggccc tcacccggca ccaagaaacc cacacccagg
     6541 aaaaaccccc caatcccgag gacccccctc cagaggcagt caccctgtcc acagatcagg
     6601 aaggtgaggg cgagacccct acccccacag agagcagcag ccatggggaa gggcaaaacc
     6661 ccaaaaccct agtggaagaa aagccctatc tgtgccccga gtgtggagcc ggcttcacag
     6721 aagtcgcagc cctcctgctc cataggagct gccacccagg tgtctccctg tagtaatgaa
     6781 ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
     6841 agaatttcca gctcttggaa tggccacgcg ttaagtcgac aatcaacctc tggattacaa
     6901 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6961 cgctgcttta atgccttkgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7021 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7081 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7141 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7201 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7261 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7321 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7381 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7441 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     7501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7561 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7741 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     7801 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     7861 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7921 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7981 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8041 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8101 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     8161 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     8221 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     8281 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     8341 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8401 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8461 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8521 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8581 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8641 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8701 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8761 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     8821 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8881 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8941 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9001 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9061 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9121 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9181 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9241 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9301 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9361 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9421 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9481 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9541 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9601 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9661 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9721 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9781 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9841 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9901 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9961 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10021 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10081 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10141 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10201 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10261 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10321 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10381 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    10441 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    10501 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10561 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10621 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10681 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10741 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    10801 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    10861 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    10921 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgcca
//
