LOCUS       sequence-408891-        9976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0720
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..233
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    461..586
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1079..1312
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1497..1541
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1772..1982
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1794..1990
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1841..1976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2022..2624
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2022..2041)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2518..2538
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2667..2784
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2922..4100
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3153..4091
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4048..4068
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5835..6423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5888..5908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6306..6317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6495..6728
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6806..6927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6843..6862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6897..6916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6967..7102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7122..7141)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7123..7141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7151..7168)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7151..7167)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7160..7182)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7309..7764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7396..7415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7606..7627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7790..7894
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7895..7963,7964..8755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8113..8132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8926..9514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9415..9434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9668..9685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9802..9823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9838..9843,9844..9861,9862..9868)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9876..9892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9881..9903
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9900..9916
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9900..9916
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9935..9955
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9937..9955
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
       61 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
      121 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
      181 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
      541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
      601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
      661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
      721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
      781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
      841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
      901 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
      961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1681 cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc
     1741 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct
     1801 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1921 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1981 cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca
     2041 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     2101 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     2161 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     2221 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2281 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     2341 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     2401 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     2461 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     2521 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     2581 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg
     2641 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2701 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2761 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     2821 cgccggctcg aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt
     2881 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     2941 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     3001 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     3061 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     3121 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     3181 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     3241 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     3301 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     3361 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     3421 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     3481 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     3541 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     3601 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     3661 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     3721 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     3781 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     3841 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     3901 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     3961 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     4021 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     4081 ttccatttca ggtgtcgtga ggctagcacc gccaccatga ttttgttgtc ttttgtttca
     4141 gattctaatg taggaactgg tgagaagaag gtgactgaag cctggatttc tgaggatgaa
     4201 aactcacata ggacgacgtc agacagactc acggtgatgg agctcccctc tcccgagtct
     4261 gaggaagtcc acgagcccag attaggggag ctcttgggaa atccagaagg tcagagcctg
     4321 gggagttccc cctctcagga caggggctgc aagcaggtga cagtgaccca ttggaagatc
     4381 cagacaggag agacagctca agtgtgcacc aagtcaggaa gaaaccatat tctgaactca
     4441 gaccttcttc tgcttcagag agagctcata gagggggaag ccaatccttg cgatatctgt
     4501 ggcaaaacct tcacgtttaa ttcggaccta gttaggcatc ggatttcgca tgctggggag
     4561 aaaccttaca cgtgcgatca gtgtgggaaa ggctttggcc agagctcaca ccttatggag
     4621 catcagagaa ttcacactgg agagagactc tacgtctgta atgtgtgtgg gaaagacttc
     4681 attcactatt caggtctcat tgagcatcag cgcgttcatt caggagaaaa gcccttcaaa
     4741 tgtgcgcagt gtgggaaggc gttttgtcac agttcagacc tgattaggca ccagagagtt
     4801 cacaccagag agagaccttt tgaatgcaaa gagtgtggga aaggcttcag tcagagctcc
     4861 ttacttattc gccatcagag gattcacacg ggagaaaggc cctatgagtg caatgaatgt
     4921 gggaaatcct tcataaggag ctcgagcctc attcgccatt atcagatcca cacagaagtg
     4981 aaacagtatg aatgcaaaga atgtgggaag gcattccgtc atcgctcaga ccttattgaa
     5041 caccagagaa ttcacaccgg agagagaccc tttgaatgca atgagtgtgg gaaagccttt
     5101 attcggagtt caaagctcat tcagcatcag aggatccata ctggggagag gccttacgta
     5161 tgcaatgagt gtgggaagcg cttcagccag acgtcaaact tcacccagca tcagagaatt
     5221 cacactggag agaaactcta tgaatgtaac gagtgtggga aagctttctt tctgagttca
     5281 taccttattc gacaccagaa aatccacact ggagagagag tgtatgaatg taaggaatgt
     5341 gggaaagcgt ttctccagaa agcccatctc actgagcacc agaagatcca ctctggggac
     5401 aggcccttcg aatgtaaaga ctgtgggaaa gccttcatcc agagctccaa gctgcttctg
     5461 caccagatta ttcacactgg agaaaagccc tatgtgtgca gttattgtgg gaaaggcttt
     5521 attcagaggt caaacttcct tcaacaccag aaaattcata ctgaagagaa gctctatgaa
     5581 tgtagtcagt atgggagaga ttttaactca actacaaacg ttaaaaataa tcaaagggtt
     5641 caccaagagg gactctcctt gagtaaggcc cccatacatt tgggtgagag gtctgtagat
     5701 aagggggaac acacaggtaa cttatagtaa tgaactagtc cgtaacttga aagtatttcg
     5761 atttcttggc tttatatatc ttgtggaaag gacgagagga aacacatcga cctggcctta
     5821 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5881 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5941 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6001 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6061 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6121 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6181 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6241 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6301 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6361 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6421 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6481 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6541 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6601 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6661 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6721 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     6781 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     6841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6901 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     6961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7021 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7081 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     7141 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7201 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7261 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7381 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7501 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7621 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7681 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7741 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7801 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7861 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7921 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7981 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8041 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8101 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8161 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8221 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8281 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8341 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8401 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8461 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8521 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8581 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8641 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8701 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8761 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8821 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8881 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8941 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9001 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9061 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9601 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9661 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9721 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9781 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9841 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9901 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9961 aaagctggag ctgcaa
//
