LOCUS       sequence-408893-        9850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9850)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0727
FEATURES             Location/Qualifiers
     source          1..9850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..237
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             238..418
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    465..590
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1083..1316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1501..1545
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1776..1986
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1798..1994
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1845..1980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2026..2628
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2026..2045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2522..2542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2671..2788
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2926..4104
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3157..4095
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4052..4072
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5713..6301
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5766..5786)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6184..6195)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6373..6606
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6684..6805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6721..6740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6775..6794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6845..6980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7000..7019)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7001..7019)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7029..7046)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7029..7045)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7038..7060)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7187..7642
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7274..7293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7484..7505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7668..7772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7773..7841,7842..8633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7991..8010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8804..9392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9293..9312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9546..9563
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9680..9701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9716..9721,9722..9739,9740..9746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9754..9770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9759..9781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9778..9794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9778..9794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9813..9833
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9815..9833
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
       61 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      121 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      181 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      421 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      481 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      541 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      601 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      661 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      721 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      781 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      841 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      901 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      961 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1021 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1081 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1141 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1201 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1261 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1321 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1381 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1441 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1501 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1561 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1621 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1681 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     1741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     1801 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     1861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     1921 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     1981 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     2041 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2101 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2161 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2221 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2281 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2341 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     2401 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     2461 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     2521 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     2581 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     2641 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2701 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2761 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     2821 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     2881 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2941 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3001 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3061 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3121 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3181 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3241 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3301 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3361 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3421 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3481 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3541 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3601 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3661 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3721 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3781 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3841 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3901 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3961 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4021 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4081 tttcttccat ttcaggtgtc gtgaggctag caccgccacc atgtcgacca cacttctgtc
     4141 cgccttctac gatgtcgact tcttgtgcaa gacagagaaa tccctggcca acctcaacct
     4201 gaacaacatg ctggacaaga aggcggtggg gacgcctgtg gccgccgccc ccagctcggg
     4261 cttcgcgccg ggattcctcc gacggcactc ggccagcaac ctgcatgcac tcgcccaccc
     4321 cgcgcccagc cccggcagct gctcgcccaa gttcccgggc gccgctaacg gcagcagctg
     4381 cggcagcgcg gcggccggcg gtccgacctc ctacggcacc cttaaggagc cgtcgggggg
     4441 cggcggcaca gccctgctca acaaggagaa caaattccgg gaccgctcgt ttagcgagaa
     4501 cggcgatcgc agccagcacc tcctgcacct gcagcagcag cagaaggggg gcggcggctc
     4561 ccagatcaac tccacgcgct acaagaccga gctgtgccgg cccttcgagg agagcggcac
     4621 gtgcaagtac ggcgaaaagt gccagttcgc gcatggcttc cacgagctgc gcagcctgac
     4681 tcgccatccg aagtacaaga ccgagctgtg ccgcaccttt cataccatcg gcttctgccc
     4741 ctatgggccg cgctgccact tcatccacaa cgcggacgag cggcggcccg cgccgtcggg
     4801 gggcgcctcc ggggacctgc gtgcctttgg cacgcgcgat gcgttgcacc tgggcttccc
     4861 gcgggagccg cggcccaagt tgcaccacag cctcagcttc tcgggcttcc cgtcgggcca
     4921 ccatcagccc ccgggcggcc tcgagtcgcc gctgctgctc gacagcccca cgtcgcgcac
     4981 gccgccgccg ccctcctgct cttcggcctc gtcctgctcc tcctccgcct cctcctgttc
     5041 ctcggcctcc gcggcctcca cgccctcggg cgccccgaca tgctgcgcct ccgcggcggc
     5101 cgcggctgcg gccgctctgc tgtacggcac cgggggcgcc gaggacctgc tggcgccggg
     5161 ggccccgtgc gcggcctgct cgtcggcctc gtgcgccaac aacgccttcg ccttcggtcc
     5221 ggagctcagc agcctcatca cgccgctcgc catccagacc cacaactttg ccgccgtggc
     5281 cgccgccgcc tactaccgca gtcagcagca gcagcagcag cagggcctgg cgccccccgc
     5341 gcagccgccg gcgccgccca gcgcgaccct ccccgccggg gccgccgcac ctccctcgcc
     5401 gcccttcagc ttccagctgc cgcgccgcct gtccgactcg cccgtgttcg acgcgccccc
     5461 cagccccccg gactcgctgt cggaccgcga cagctaccta agcggctccc tgagctccgg
     5521 cagcctcagc ggctctgagt ctcccagcct cgaccctggc cgccgcctgc caatcttcag
     5581 ccgcctctcc atctccgacg actagtaatg aactagtccg taacttgaaa gtatttcgat
     5641 ttcttggctt tatatatctt gtggaaagga cgacggcaaa cttaactgta cctggtgacg
     5701 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5761 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5821 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5881 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5941 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6001 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6061 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6121 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6181 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6241 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6301 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6361 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6421 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6481 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6541 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6601 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6661 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6781 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6841 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6901 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6961 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     7021 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     7081 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     7141 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     7201 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7261 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7321 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7381 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7441 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7501 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7561 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7621 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7981 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     8041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9421 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9481 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9541 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9601 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9661 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9721 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9781 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     9841 agctggagct
//
