LOCUS       sequence-408894-       10144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0732
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        33..259
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             260..440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    487..612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1105..1338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1523..1567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1798..2008
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1820..2016
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1867..2002
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2048..2650
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2048..2067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2544..2564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2693..2810
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2948..4126
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3179..4117
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4074..4094
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4137..4146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6029..6617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6082..6102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6500..6511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6689..6922
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7000..7121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7037..7056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7091..7110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7161..7296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7316..7335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7317..7335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7345..7362)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7345..7361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7354..7376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7503..7958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7590..7609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7800..7821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7984..8088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8089..8157,8158..8949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8307..8326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9120..9708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9609..9628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9862..9879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9996..10017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10032..10037,10038..10055,10056..10062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10070..10086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10075..10097
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10094..10110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10094..10110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(10129..10144,1..5)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(10131..10144,1..5)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
       61 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
      121 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
      181 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
      241 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
      301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
      361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
      421 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
      481 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
      541 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
      601 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
      661 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
      721 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
      781 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
      841 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
      901 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
      961 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     1021 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     1081 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     1141 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     1201 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     1261 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     1321 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     1381 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     1441 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     1501 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     1561 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     1621 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     1681 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     1741 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     1801 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
     1861 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     1921 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     1981 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
     2041 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     2101 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     2161 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     2221 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     2281 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2341 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2401 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2461 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2521 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2581 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     2641 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     2701 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2761 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2821 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
     2881 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     2941 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     3001 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     3061 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     3121 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     3181 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     3241 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     3301 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     3361 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     3421 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     3481 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     3541 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     3601 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     3661 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     3721 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     3781 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     3841 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     3901 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     3961 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     4021 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     4081 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggct agcaccgcca
     4141 ccatggatcc aaactccatc ttgctttctc ctcagcccca gatctgctcc cacctagcag
     4201 aagcttgtac ggaaggcgag agaagctcat cccctccaga gctggataga gactccccgt
     4261 ttccctggag tcaggtcccc agctccagcc ctacagaccc cgaatggttt ggtgatgagc
     4321 acatccaggc aaagagggcc agagtggaga ccattgtccg aggcatgtgt ctctccccga
     4381 atcctctggt gccaggcaat gcgcaagctg gggtcagccc acgctgccca aagaaggccc
     4441 gagagaggaa gaggaagcag aaccttccca caccgcaagg cctcctgatg ccagcccctg
     4501 cctgggacca gggcaacagg aaggggggcc ctcgtgtgag agaacaactt catctgctga
     4561 agcaacagct aagacatctg caagagcaca tcctacaggc tgccaagccc agggacacag
     4621 ctcaggggcc aggaggctgt ggcacgggga aaggccctct gagtgcaaag caggggaatg
     4681 gctgtgggcc tcgcccctgg gttgtggacg gtgaccacca gcaaggtacc agcaaggacc
     4741 tctctggggc agaaaaacac caagagtctg agaagcccag tttccttcct tctggagcac
     4801 cagcttcact agagattctg aggaaagagc tgaccagggc agtgtcccag gctgtggact
     4861 cggtattaca aaaggtacta ttggatccac caggccacct gactcagctg ggcagaagct
     4921 tccaggggca ggtggcagag ggtagaagcg agccctcacc tcctgtggga ggggcctgta
     4981 aagatccact tgctttggct gccttgccca ggagggtcca gctacaagct ggggtcccag
     5041 taggaaattt atcactggcc aagcgtctag attctcctag gtaccctatc cctccaagaa
     5101 tgacccccaa accctgtcag gatcccccag caaactttcc cttgactgca ccttcccaca
     5161 tccaggaaaa tcagattctt agccagctac tgggtcatag atacaacaat ggccattgga
     5221 gtagcagtcc tccccaggac tcatcttccc agaggcaccc ctcctcagag cctgccctac
     5281 gaccttggag aactactaag ccgcaaccat tggtcctgag ccagcagcag tgtcccttgc
     5341 ctttcacctc tgcccatctg gaaagtctac cccttcttcc ctcggtgaag atggaacaga
     5401 gaggcctgca tgctgtcatg gaggcactgc ctttctcttt ggtccacatc caggagggtc
     5461 taaaccctgg tcacttgaag aaggccaaac taatgttttt cttcacacga tatcccagct
     5521 ccaacctcct gaaggtttat tttcctgatg ttcagttcaa ccgctgcatt acctcccaga
     5581 tgatcaagtg gttcagcaac tttcgtgagt tttattacat ccaaatggaa aaatctgccc
     5641 ggcaagcaat ttcagatggt gtcacaaatc ccaaaatgct ggtggttctc cgcaattcag
     5701 aactttttca agctctcaat atgcactaca acaagggaaa tgactttgag gttccagatt
     5761 gcttcttgga aattgccagc ttgacgttac aggagttctt cagggctgtc tccgcaggga
     5821 gagactcaga tccttcctgg aagaaaccca tttataaaat tatttcgaaa ctggacagtg
     5881 acatcccaga gatattcaaa tcttccagct atccccagta gtaatgaact agtccgtaac
     5941 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac accaacggct
     6001 catcattagc ccaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6061 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6121 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6181 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6241 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6301 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6361 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6421 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6481 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6541 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6601 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     6661 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6721 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6781 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6841 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6901 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     6961 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7081 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7141 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7261 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     7321 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7381 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7441 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7501 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7861 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7921 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     7981 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8101 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8161 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8221 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8281 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8341 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8401 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8461 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8521 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8581 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8641 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8701 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8761 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8821 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8881 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9781 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9841 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9901 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9961 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    10021 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    10081 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    10141 cact
//
