LOCUS       sequence-408899-       11359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of human CD47.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-hCD47-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        208..587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        589..787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             805..985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1032..1157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1650..1883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2068..2112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2410..2527
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2580..2598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2616..2634
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2651..2669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2687..2705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2723..2741
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2794..2812
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2824..2862
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2859..2883
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2958..4169
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4006..4025
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5184..5237
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5241..5243,5244..5957)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5283..5304)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5544..5563)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5891..5912
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6017..6605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6070..6090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6488..6499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6608..6624)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6609..6625)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7134..7314
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7340..7357)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7346..7570
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7616..8044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7703..7722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7913..7934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8053..8073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8058..8387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8238..8373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8300..8319
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8435..8482
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8501..8875
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9005..9126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9042..9061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9096..9115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9175..9191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9175..9191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9188..9210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9199..9215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9223..9229,9230..9247,9248..9253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9268..9289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9406..9423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9577..10165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9657..9676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10336..11127,11128..11196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10959..10978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11197..11301)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
       61 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      181 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      601 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      721 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      781 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      961 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1021 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1081 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1141 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1201 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1261 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1321 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1381 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1441 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1501 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1561 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1621 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1681 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1741 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1801 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1861 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1921 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1981 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2041 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2101 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2161 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2221 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2281 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2341 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     2401 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2461 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2521 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaattc tagttttact
     2581 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     2641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     2701 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     2761 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     2821 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     2881 atcgcctgga gaattcgtta acggatcctc tagagctcag cctcgagatt taaatttaat
     2941 taacccaccc aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     3001 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     3061 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     3121 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     3181 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     3241 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     3301 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
     3361 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
     3421 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
     3481 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     3541 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     3601 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     3661 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     3721 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     3781 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     3841 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     3901 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     3961 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     4021 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     4081 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     4141 tctggccgtt tttggctttt ttgttagacg aagcttggta ccgagctcgg atctccaccc
     4201 cgtaccggtt aaccatgtgg cctctcgtcg ctgccctgct gctgggatcc gcctgctgtg
     4261 gatccgccca gctcctgttt aataaaacaa agagcgtgga gtttaccttt tgtaacgata
     4321 ccgtggtgat cccctgcttc gtcaccaaca tggaagccca gaataccacc gaagtgtatg
     4381 tcaagtggaa gttcaaaggc agggacattt acaccttcga tggcgctctg aacaaaagca
     4441 ccgtgcccac agatttcagc tccgccaaga tcgaagtgtc ccagctgctg aagggcgatg
     4501 ctagcctgaa aatggacaaa tccgacgccg tgagccacac cggcaactat acctgcgagg
     4561 tcacagaact gacaagggaa ggcgagacaa tcatcgagct gaaatacaga gtggtgtcct
     4621 ggttctcccc taacgagaac attctgatcg tgattttccc tatctttgcc attctgctgt
     4681 tctggggcca gtttggcatc aagaccctga agtatagatc cggcggcatg gatgagaaaa
     4741 ccattgccct cctggtggct ggcctggtca ttaccgtgat cgtgatcgtg ggcgctatcc
     4801 tcttcgtccc tggcgagtac agcctgaaaa acgccaccgg actgggactg atcgtgacct
     4861 ccacaggaat cctgattctg ctgcactatt acgtgtttag caccgctatc ggcctgacct
     4921 cctttgtgat cgccatcctg gtgatccagg tcatcgctta tatcctggcc gtcgtcggac
     4981 tgagcctctg tatcgccgcc tgtatcccca tgcatggccc cctcctcatc agcggactct
     5041 ccattctggc cctggcccaa ctcctgggcc tcgtgtacat gaagttcgtg gcctccaacc
     5101 aaaaaacaat ccagcctccc agaaaggccg tcgaggagcc cctgaacgct tttaaggaga
     5161 gcaaaggcat gatgaacgat gaggagggca gaggaagtct tctaacatgc ggtgacgtgg
     5221 aggagaatcc cggccctgtg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5281 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5341 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     5401 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
     5461 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     5521 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     5581 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     5641 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
     5701 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
     5761 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     5821 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5881 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5941 acgagctgta caagtaatgt acaagtaaag cggccgcgac tctagtccct cccaattcga
     6001 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6061 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6121 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6181 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6241 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6301 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6361 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6421 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     6481 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6541 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6601 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
     6661 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     6721 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     6781 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6841 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     6901 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     6961 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     7021 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     7081 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     7141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     7321 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7381 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7441 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     7501 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     7561 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     7621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     7861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     7921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     7981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8041 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8101 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8161 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8221 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8281 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     8341 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     8401 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     8461 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     8521 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     8581 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     8641 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     8701 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     8761 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     8821 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     8881 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8941 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     9001 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     9061 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9121 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     9241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     9301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     9361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     9421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     9481 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     9541 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     9601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    10261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    10321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    10381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    10441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    10501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    10561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    10621 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10921 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    11221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    11281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    11341 gggagatctc ccgatcccc
//
