LOCUS       sequence-408917-        9388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0734
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..257
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             258..438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    485..610
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1103..1336
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1521..1565
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1796..2006
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1818..2014
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1865..2000
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2046..2648
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2046..2065)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2542..2562
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2691..2808
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2946..4124
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3177..4115
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4072..4092
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5271..5859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5324..5344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5742..5753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5931..6164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6242..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6333..6352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6403..6538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6558..6577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6559..6577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6587..6604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6587..6603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6596..6618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6745..7200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6832..6851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7042..7063
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7226..7330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7331..7399,7400..8191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7549..7568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8362..8950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8851..8870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9104..9121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9238..9259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9274..9279,9280..9297,9298..9304)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9312..9328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9317..9339
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9336..9352
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9336..9352
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(9371..9388,1..3)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(9373..9388,1..3)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
       61 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      121 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      181 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      241 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      301 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      361 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      421 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      481 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      541 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      601 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      661 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      721 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      781 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      841 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      901 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
      961 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1021 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1081 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1141 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1201 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1261 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1321 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1381 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1441 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1501 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1561 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1621 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1681 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca
     1741 tgcattgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     1801 cagaagtatg caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc
     1861 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     1921 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     1981 aggaggcttt tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc
     2041 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     2101 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     2161 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     2221 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc
     2281 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     2341 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     2401 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     2461 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2521 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2581 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     2641 ccagttaacg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
     2701 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2761 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     2821 agggacagca gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa
     2881 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     2941 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     3001 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     3061 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3121 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3181 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3241 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     3301 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     3361 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     3421 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     3481 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     3541 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     3601 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     3661 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     3721 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     3781 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     3841 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3901 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     3961 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     4021 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     4081 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag caccgccacc
     4141 atgccagagt ggccaccttg cttatctgtg gccccggccc tggtgatcac catggcagct
     4201 gggaagggag ccccgttgag cccatcggct gaaaacagat ggcgacttag cgaacctgag
     4261 ctgggccggg gctgcaagcc agtgctgctc gagaagacga accgcctggg ccctgaggct
     4321 gctgtgggca gggcgggccg ggatgtgggc agtgcggagc tggcactgtt ggtagcccca
     4381 ggcaagcccc gacctggcaa gccgctgccc ccgaagacac gtggagagca gaggcagagc
     4441 gccttcacgg agctgccgag gatgaaggac cggcaggtgg atgctcaggc ccaggagagg
     4501 gagcacgatg accccacagg ccaacctggt gccccacagc tgacccagaa catccccaga
     4561 ggcccagctg gcagcaaagt cttctctgtg tggcccagcg gagcacgaag tgagcaaaga
     4621 agcgccttta gcaaaccaac caagcgacca gcagagaggc ctgagctaac ctcagtcttc
     4681 cctgcagggg aatctgcaga tgccctgggg gagctgtctg gactcctcaa cactacagac
     4741 ctcgcttgtt ggggtcgact ttcaactccc aagcttttgg ttggtgattt gtggaacttg
     4801 caagcattgc cacagaatgc tccactctgt agcacttttc tgggtgcccc tacactgtgg
     4861 ctggagcata cccaggccca ggtgccccca ccctcatcat cctccaccac ttcgtgggcc
     4921 ctcctgccac ccaccctcac ctccctgggc ttgtccaccc agaactggtg tgcaaagtgc
     4981 aacctgtcct ttcgcctaac gtccgacctg gtctttcaca tgcgatccca ccacaaaaag
     5041 gagcatgcgg ggcctgaccc acattctcag aagcggagag aagaggccct tgcctgccct
     5101 gtgtgccagg agcacttccg ggagcgccac cacctctccc ggcacatgac ttctcacagc
     5161 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     5221 ggaaaggacg aggttcgcgg gcgactcaca attatacgcg ttaagtcgac aatcaacctc
     5281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5821 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     5881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5941 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     6181 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6241 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6361 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6541 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6601 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6661 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6721 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6781 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6841 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6901 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6961 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7021 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7081 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7141 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7201 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7261 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7321 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7381 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7441 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7501 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7561 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7621 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7681 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7741 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7801 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7861 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7921 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7981 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8041 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8101 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8161 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9061 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9121 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9301 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9361 cgccaagcgc gcaattaacc ctcactaa
//
