LOCUS       sequence-408918-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0742
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..246
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             247..427
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    474..599
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1092..1325
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1510..1554
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1785..1995
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1807..2003
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1854..1989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2035..2637
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2035..2054)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2531..2551
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2680..2797
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2935..4113
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3166..4104
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4061..4081
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4124..4133
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5563..6151
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5616..5636)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6034..6045)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6223..6456
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6534..6655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6571..6590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6625..6644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6695..6830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6850..6869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6851..6869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6879..6896)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6879..6895)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6888..6910)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7037..7492
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7124..7143)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7334..7355
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7518..7622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7623..7691,7692..8483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7841..7860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8654..9242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9143..9162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9396..9413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9530..9551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9566..9571,9572..9589,9590..9596)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9604..9620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9609..9631
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9628..9644
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9628..9644
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9663..9683
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9665..9683
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
       61 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      121 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      181 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      241 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      301 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      361 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      421 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      481 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      541 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      601 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      661 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
      721 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
      781 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
      841 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
      901 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
      961 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1021 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1081 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1141 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1201 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1261 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1321 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1381 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1441 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1501 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1561 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1621 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1681 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat gcattgcatc
     1741 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1801 aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     1861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1921 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1981 ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct taccatgacc
     2041 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     2101 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     2161 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     2221 aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc
     2281 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2341 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2401 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2461 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2521 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2581 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cagttaacga
     2641 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2701 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2761 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2821 agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa gttttaaaca
     2881 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     2941 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     3001 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     3061 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     3121 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     3181 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     3241 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     3301 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     3361 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     3421 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     3481 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     3541 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3601 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3661 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3721 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     3781 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     3841 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3901 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3961 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     4021 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     4081 caaagttttt ttcttccatt tcaggtgtcg tgaggctagc accgccacca tggccccaag
     4141 agcccagatc cagggaccgc tgacatttgg ggatgtggct gtggccttta cccggattga
     4201 gtggagacac ctggatgctg ctcagcgggc cctgtacagg gatgtgatgc tggagaacta
     4261 tgggaatctg gtgtctgtgg ggcttctctc ttccaaacca aaactaatta ctcagttgga
     4321 gcaaggggca gagccctgga ctgaggtgcg agaggctcca tcaggcacac atgcagtcga
     4381 ggattactgg tttgaaacaa agatgtcagc cctaaagcaa agcacttctg aagcatctgt
     4441 tctgggagag cgaacgaaaa gtgtcatgat ggaaaaaggc ctggactggg agggcagaag
     4501 ctccacagag aagaactata agtgcaagga atgtgggaaa gtcttcaaat acaattcgtc
     4561 ctttattagc caccagagaa atcacaccag tgagaaacca cataaatgta aagaatgtgg
     4621 gatcgccttt atgaacagtt catccctttt aaatcaccat aaggttcatg caggcaaaca
     4681 gccttataga tgtattgaat gtgggaagtt cctgaagaag cactcaacgt ttatcaacca
     4741 tcagagaatt cattctaggg agaaacccca caaatgcatt gaatgtggaa aaactttcag
     4801 aaagaactca atccttttaa gtcatcagag aattcatact ggccagaaac cctacaaatg
     4861 taatgactgt gggaaagcct tcgctcagaa tgcagctctt actcgtcatg aaagaataca
     4921 tagtggagag aagcctttta aatgtaacaa gtgtgggaga gctttcaggg ataattcaac
     4981 tgtgttggaa catcagaaaa tccatactgg tgagaagcca tatcagtgta atgaatgtgg
     5041 aaaagccttt aggaagagct caactcttat tagtcaccaa agaatgcata ctggggagaa
     5101 accctatcac tgcagtaaat gtggaaaatc tttcaggtat agctcatcct ttgctggtca
     5161 tcagaaaact cacagtggaa ataaaccgta tcagtgtcgt gactgtggga aggcctttac
     5221 aaagagctca acccttactg gacatcagag aattcatact ggagaaaaac cctatcactg
     5281 taagaaatgt gggaaagcct ttcggcacag ctcaggcctt gttgaacatc agagactcca
     5341 tactggggaa aaaccttata aatgtaatga atgtgggaaa gcttttcccc gaagttcagc
     5401 ccttaagcaa cataagaaaa ttcataataa agaaagagcc atgaaatgta gttagtaatg
     5461 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     5521 cgagagggca aatcttgcaa atccgctacg cgttaagtcg acaatcaacc tctggattac
     5581 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     5641 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     5701 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     5761 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5821 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5881 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5941 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6001 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6061 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6121 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     6181 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     6241 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     6301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     6481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6661 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6721 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6841 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6901 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6961 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     7021 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     7081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     7141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7441 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     7501 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7861 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8761 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     9481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     9541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     9601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     9661 gcgcaattaa ccctcactaa agggaacaaa a
//
