LOCUS       sequence-408919-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of human CD46.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-hCD46-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(98..202)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(277..296)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        468..847
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        849..1047
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1065..1245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1292..1417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1910..2143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2328..2372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2670..2787
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2840..2858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2876..2894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2911..2929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2947..2965
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2983..3001
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3018..3036
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3054..3072
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3084..3122
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3119..3143
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3218..4429
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4266..4285
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5606..5659
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5663..5665,5666..6379)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5705..5726)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5966..5985)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6313..6334
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6439..7027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6492..6512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6910..6921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7030..7046)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7031..7047)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7556..7736
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7762..7779)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7768..7992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8038..8466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8125..8144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8335..8356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8475..8495)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8480..8809
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8660..8795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8722..8741
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8857..8904
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8923..9297
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9427..9548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9464..9483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9518..9537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9597..9613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9597..9613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9610..9632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9621..9637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9645..9651,9652..9669,9670..9675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9690..9711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9828..9845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9999..10587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10079..10098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10758..11521,1..28,29..97))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11381..11400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
       61 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
      121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
      181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
      241 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      301 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      361 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      421 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      481 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      541 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      601 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      661 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      721 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      781 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      841 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      901 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      961 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1021 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     1081 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     1141 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     1201 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     1261 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     1321 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     1381 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1441 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1501 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1561 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1621 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1681 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1741 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1801 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1861 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1921 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1981 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     2041 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     2101 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     2161 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     2221 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     2281 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     2341 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     2401 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2461 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2521 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2581 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2641 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2701 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2761 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2821 tggttaattc tagttttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     2881 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     2941 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     3001 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     3061 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     3121 ctcgtttagt gaaccgtcag atcgcctgga gaattcgtta acggatcctc tagagctcag
     3181 cctcgagatt taaatttaat taacccaccc aagatctggc ctccgcgccg ggttttggcg
     3241 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     3301 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     3361 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     3421 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     3481 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     3541 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     3601 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     3661 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     3721 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     3781 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     3841 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     3901 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     3961 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     4021 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     4081 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     4141 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     4201 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     4261 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     4321 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     4381 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttggta
     4441 ccgagctcgg atctccaccc cgtaccggtt aaccatggaa ccccctggaa ggagagagtg
     4501 ccctttccct tcctggagat ttcccggact gctgctggct gccatggtcc tgctgctgta
     4561 cagcttcagc gatgcttgtg aggagcctcc cacattcgag gccatggaac tcattggcaa
     4621 gcctaagccc tactacgaaa tcggcgagag ggtcgattac aagtgtaaga agggctattt
     4681 ctacatcccc cccctggcca cacacaccat ctgcgataga aaccacacat ggctccctgt
     4741 gtccgacgat gcttgctata gggagacctg cccttacatc agggaccctc tgaatggaca
     4801 ggccgtgcct gctaatggca cctacgaatt cggctatcaa atgcatttca tctgcaatga
     4861 gggctactac ctgatcggcg aagagattct ctactgtgag ctcaagggaa gcgtcgctat
     4921 ctggagcgga aagcctccca tctgcgagaa agtgctgtgc accccccccc ctaaaatcaa
     4981 aaatggcaag cacaccttca gcgaggtgga agtgttcgaa tatctggatg ccgtgaccta
     5041 cagctgtgat cctgctcctg gacccgatcc ctttagcctg attggcgaga gcacaattta
     5101 ctgcggagac aactccgtgt ggagcagagc cgcccccgag tgtaaggtcg tgaaatgtag
     5161 gttccctgtc gtcgagaatg gcaaacagat cagcggcttc ggcaaaaagt tctactataa
     5221 agctaccgtg atgtttgagt gcgacaaggg attctacctc gatggcagcg atacaatcgt
     5281 gtgcgatagc aactccacat gggacccccc tgtccccaaa tgcctgaaag tgagcaccag
     5341 ctccacaacc aagtcccccg cctcctccgc ctccggcccc agacctacct ataagccccc
     5401 tgtgtccaac taccccggct accccaaacc cgaggaaggc attctggact ccctggatgt
     5461 gtgggtgatt gccgtcatcg tcattgctat tgtggtcgga gtcgctgtga tctgtgtcgt
     5521 gccctataga tacctgcaga gaaggaaaaa gaagggcacc tacctgaccg acgagaccca
     5581 cagagaagtg aagtttacca gcctggaggg cagaggaagt cttctaacat gcggtgacgt
     5641 ggaggagaat cccggccctg tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5701 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5761 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5821 gcccgtgccc tggcccaccc tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg
     5881 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5941 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6001 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6061 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
     6121 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
     6181 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6241 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6301 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6361 ggacgagctg tacaagtaat gtacaagtaa agcggccgcg actctagtcc ctcccaattc
     6421 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6841 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7021 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag
     7081 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7141 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7201 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7261 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     7321 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7381 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7441 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7501 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7561 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7621 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7681 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7741 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7801 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7861 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7921 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7981 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     8041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8401 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8461 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8581 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8641 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8701 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8761 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8821 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8881 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8941 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     9001 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     9061 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     9121 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     9181 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     9241 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     9301 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9361 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9421 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9481 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9541 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9661 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    11041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11521 t
//
