LOCUS       sequence-408922-       10237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10237)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0749
FEATURES             Location/Qualifiers
     source          1..10237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..252
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             253..433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    480..605
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1098..1331
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1516..1560
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1791..2001
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1813..2009
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1860..1995
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2041..2643
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2041..2060)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2537..2557
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2686..2803
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2941..4119
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3172..4110
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4067..4087
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4130..4139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6115..6703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6168..6188)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6586..6597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6775..7008
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7086..7207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7123..7142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7177..7196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7247..7382
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7402..7421)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7403..7421)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7431..7448)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7431..7447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7440..7462)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7589..8044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7676..7695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7886..7907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8070..8174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8175..8243,8244..9035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8393..8412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9206..9794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9695..9714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9948..9965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10082..10103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10118..10123,10124..10141,10142..10148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10156..10172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10161..10183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10180..10196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10180..10196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10215..10235
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10217..10235
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
       61 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      121 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      181 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      241 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      301 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      361 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      421 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      481 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      541 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      601 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      661 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      721 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      781 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      841 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
      901 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
      961 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1021 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1081 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1141 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1201 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1261 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1321 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1381 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1441 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1501 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1561 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1621 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1681 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccatgcat
     1741 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1801 gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta actccgccca
     1861 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1921 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     1981 gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc ggagcttacc
     2041 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2101 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2161 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2221 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     2281 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2341 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2401 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     2461 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2521 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2581 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccagt
     2641 taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2701 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2761 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2821 cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg gataaagttt
     2881 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     2941 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3001 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3061 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3121 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3181 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3241 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3301 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3361 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3421 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3481 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3541 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3601 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3661 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3721 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3781 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3841 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3901 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3961 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4021 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4081 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     4141 ggctgaagag acgatggaat gccttcagga gttccctgaa catcataaaa tgatcctcga
     4201 ccgattgaat gaacagcgag agcaggaccg gtttactgac atcaccctaa ttgtcgacgg
     4261 acaccatttt aaggctcaca aggctgtttt ggctgcttgt agtaagttct tctacaaatt
     4321 ctttcaggag tttacccaag aaccattggt ggagatagaa ggtgttagta aaatggcctt
     4381 tcgccattta attgagttca catatacagc aaaattaatg atacaaggag aagaagaagc
     4441 caatgatgta tggaaagcag cagagtttct acaaatgcta gaagctatca aagcccttga
     4501 agtcaggaac aaagaaaact cagctccctt agaggaaaat accacaggaa aaaatgaggc
     4561 caaaaaaagg aagattgcag aaacttcaaa tgttatcact gagtcattgc catctgcaga
     4621 atcagaacct gttgaaattg aggtagagat tgccgaaggc accattgaag tggaagatga
     4681 aggcatcgaa acattagagg aagtggcttc tgccaagcag tccgtaaagt acatacagag
     4741 cacaggttcc tctgatgatt ctgctctagc actgttggca gatattacca gcaagtaccg
     4801 tcaaggtgac agaaaagggc agattaaaga agatggctgt ccatctgacc ccacgagcaa
     4861 acaggtagaa ggtattgaaa ttgtggaact tcagctgtca catgtgaagg acttgttcca
     4921 ttgtgagaaa tgtaaccgtt catttaaatt gttttaccat tttaaggagc acatgaaatc
     4981 acactccact gagagtttca agtgtgaaat atgcaataaa cgatatcttc gagagagcgc
     5041 atggaaacag cacctaaatt gttaccacct tgaagaaggt ggagtcagta agaagcaaag
     5101 aactgggaaa aaaattcatg tatgtcagta ctgtgagaaa cagtttgacc attttggaca
     5161 ttttaaagaa catcttcgaa aacatacagg tgaaaaacct tttgaatgtc caaattgtca
     5221 tgaacgattt gctagaaata gcactctgaa atgtcacctc actgcatgcc aaactggagt
     5281 aggggcaaaa aaaggaagga agaagctcta cgaatgccag gtctgcaaca gtgtgtttaa
     5341 cagctgggac cagttcaaag atcacttggt aatacacact ggagataaac ccaaccattg
     5401 tactttatgt gatttgtggt ttatgcaagg aaatgaatta aggaggcatc tcagtgatgc
     5461 tcacaatatt tcagagcgtc tagtaacgga agaagttctt tcagtagaaa cacgtgtgca
     5521 aactgaacct gtaacatcaa tgactattat agaacaagtt gggaaggtgc atgtgctacc
     5581 attgcttcag gttcaggtgg attcagcaca agtgactgtg gaacaagtcc atccagatct
     5641 gctccaggac agccaggtgc acgattcaca catgagtgag cttccagagc aggtccaagt
     5701 gagttatcta gaagtgggcc gaattcagac tgaagaaggt actgaagtac atgtagagga
     5761 gctgcatgtt gaacgggtca atcaaatgcc agtggaagta caaactgaac ttctagaagc
     5821 agatttggac cacgtgaccc cagaaatcat gaaccaagag gagagagagt ctagccaagc
     5881 agatgctgct gaggctgcca gggaagatca cgaagatgct gaggatttag agaccaagcc
     5941 aacagtggat tctgaagcag aaaaggcaga gaatgaggac agaacagctc tgccagtttt
     6001 agaatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     6061 ttgtggaaag gacgagtggg cagatcactg ttgtaaacga cgcgttaagt cgacaatcaa
     6121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6241 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6301 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6361 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6421 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6481 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6541 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6601 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6661 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     6721 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6781 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6841 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6901 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6961 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     7021 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7081 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7201 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7261 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7321 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7381 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7441 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7501 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7561 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7621 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7681 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7741 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7801 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7861 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7921 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7981 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8041 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8101 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8161 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8221 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8281 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8341 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8401 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8461 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8521 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8581 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8641 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8701 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8761 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8821 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8881 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8941 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9001 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9061 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9121 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9181 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9241 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9301 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9361 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9421 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9481 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9541 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9601 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9661 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9721 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9781 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9841 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9901 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9961 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10021 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10081 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10141 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10201 attacgccaa gcgcgcaatt aaccctcact aaaggga
//
