LOCUS       sequence-408923-       11553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for expression of mouse CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11553)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: TRE-UBC-mCD55-T2A-GFP
FEATURES             Location/Qualifiers
     source          1..11553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2646..2664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2681..2699
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2717..2735
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2753..2771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2788..2806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2824..2842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2854..2892
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2889..2913
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2988..4199
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4036..4055
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5408..5461
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5465..5467,5468..6181)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIKV
                     NFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5507..5528)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5768..5787)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6115..6136
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6241..6829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6294..6314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6712..6723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6832..6848)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6833..6849)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7358..7538
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7564..7581)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7570..7794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7840..8268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7927..7946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8137..8158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8277..8297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8282..8611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8462..8597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8524..8543
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8659..8706
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8725..9099
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9229..9350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9266..9285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9320..9339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9399..9415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9399..9415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9412..9434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9423..9439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9447..9453,9454..9471,9472..9477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9492..9513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9630..9647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9801..10389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9881..9900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10560..11351,11352..11420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11183..11202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11421..11525)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagttttact ccctatcagt gatagagaac gtatgtcgag
     2641 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
     2701 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2761 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
     2821 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
     2881 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcgtta acggatcctc
     2941 tagagctcag cctcgagatt taaatttaat taacccaccc aagatctggc ctccgcgccg
     3001 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3061 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3121 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3181 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3241 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3301 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3361 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3421 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3481 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3541 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3601 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3661 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3721 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3781 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3841 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3901 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3961 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     4021 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     4081 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4141 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4201 aagcttggta ccgagctcgg atctccaccc cgtaatgatc aggggaaggg cccctaggac
     4261 aaggcctagc cctcctcccc ctctcctccc tctcctgagc ctgagcctgc tgctgctgtc
     4321 ccctaccgtg agaggcgact gcggcccccc ccccgacatt cctaacgcca gacctattct
     4381 gggcaggcat agcaaatttg ccgaacaaag caaggtcgcc tacagctgca acaacggctt
     4441 caagcaggtc cccgacaaaa gcaacattgt cgtgtgcctc gagaacggcc aatggtccag
     4501 ccatgaaaca ttctgcgaaa aaagctgcgt ggctcccgag aggctgtcct tcgcctccct
     4561 gaaaaaggaa tatctgaata tgaatttctt ccctgtgggc accattgtgg agtatgagtg
     4621 taggcccgga ttcagaaagc agccccctct gcccggaaaa gctacatgtc tggaagatct
     4681 ggtgtggagc cccgtggctc aattctgcaa aaagaagagc tgtcccaatc ccaaggacct
     4741 cgataatggc cacatcaaca ttcctaccgg aatcctgttc ggcagcgaaa ttaactttag
     4801 ctgcaatccc ggatataggc tcgtcggcgt cagcagcacc ttctgttccg tgaccggaaa
     4861 caccgtggac tgggacgacg aattccccgt ctgtaccgag attcattgcc ctgagcctcc
     4921 taagattaac aatggcatca tgagaggcga aagcgacagc tacacctatt cccaggtcgt
     4981 cacatatagc tgcgacaagg gattcatcct ggtcggcaat gctagcatct attgcaccgt
     5041 gagcaaatcc gatgtgggac aatggagcag cccccctccc aggtgcatcg aaaagagcaa
     5101 agtgcccacc aaaaaaccca caatcaacgt gcctagcaca ggcacacctt ccacccctca
     5161 aaaacctaca accgagtccg tgcccaatcc tggagatcag cccacccctc agaaaccttc
     5221 cacagtcaag gtgagcgcca cccagcatgt ccctgtgacc aagacaacag tcagacatcc
     5281 tatcagaacc tccaccgaca aaggagaacc taacaccggc ggagacaggt acatttacgg
     5341 acacacctgt ctgatcacac tgacagtgct ccatgtcatg ctgtccctga tcggatacct
     5401 cacattagag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc
     5461 tgtggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5521 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5581 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5641 cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     5701 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5761 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5821 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5881 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     5941 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     6001 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6061 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6121 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     6181 atgtacaaat aaagcggccg cgactctagt ccctcccaat tcgatatcaa gcttatcgat
     6241 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6301 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6361 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6421 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6481 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6541 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6601 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6661 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6721 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6781 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     6841 cgacctcgat cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     6901 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     6961 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7021 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7081 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     7141 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     7201 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     7261 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     7321 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     7381 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7441 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7501 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     7561 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     7621 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     7681 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     7741 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     7801 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     7861 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7921 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7981 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8041 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8101 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8161 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8221 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     8281 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8341 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8401 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8461 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8521 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     8581 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     8641 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     8701 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     8761 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     8821 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     8881 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     8941 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9001 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9061 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     9121 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9181 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     9361 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     9421 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9481 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9541 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9601 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9661 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9721 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9781 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9841 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9901 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9961 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10021 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10081 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10141 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10201 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10261 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10321 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10381 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10441 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10501 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10561 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10621 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10681 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10741 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10801 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10861 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10921 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10981 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11041 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11101 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11161 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11221 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11281 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11341 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11401 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11461 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11521 ccgcgcacat ttccccgaaa agtgccacct gac
//
