LOCUS       sequence-408926-        3930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC46A1_STOP
FEATURES             Location/Qualifiers
     source          1..3930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1487..1586)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1603..1622)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1604..1622)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1627..1643)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1627..1643)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1745..1764)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1756..2565
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1823..1842)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2712..3300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3201..3220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3454..3471
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3630..3657)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3749..3835)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3884..3906
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3898..3915
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3899..3915
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gggatctgcc
      121 agcccacctg agaagccaag ggcaaggcca gcagcagccg tgctgtgcag gggccctgtg
      181 gagccactgg tgttcctggc caactttgcc ctggtgctgc agggcccact gaccacacag
      241 tacctgtggc accggttcag cgccgacctg ggatataacg gaaccaggca gagaggcggc
      301 tgtagcaata gatccgccga tcccaccatg caggaggtgg agaccctgac atcccactgg
      361 acactgtaca tgaatgtggg cggcttcctg gtgggcctgt ttagctccac actgctggga
      421 gcatggtccg actctgtggg ccggagacct ctgctggtgc tggcaagcct gggactgctg
      481 ctgcaggccc tggtgtccgt gttcgtggtg cagctgcagc tgcacgtggg ctattttgtg
      541 ctgggcagaa tcctgtgcgc actgctgggc gacttcggag gactgctggc agcatctttt
      601 gccagcgtgg cagacgtgag cagctccagg agccgcacct tccggatggc actgctggag
      661 gcatccatcg gagtggcagg aatgctggca tctctgctgg gaggacactg gctgagagca
      721 cagggctacg ccaacccctt ttggctggcc ctggccctgc tgatcgccat gaccctgtat
      781 gccgccttct gttttggcga gacactgaag gagcctaagt ctaccaggct gttcacattt
      841 cggcaccaca gaagcatcgt gcagctgtac gtggcaccag caccagagaa gtctcgcaag
      901 cacctggccc tgtatagcct ggccatcttc gtggtcatca ccgtgcactt tggcgcccag
      961 gacatcctga ccctgtacga gctgagcaca cctctgtgct gggattccaa gctgatcgga
     1021 tacggatctg ccgcacagca cctgccatat ctgacaagcc tgctggccct gaagctgctg
     1081 cagtactgtc tggcagacgc atgggtggca gagatcggac tggccttcaa catcctgggc
     1141 atggtggtgt tcgcctttgc caccatcaca ccactgatgt tcaccggcta tggcctgctg
     1201 tttctgtccc tggtcatcac acccgtgatc agggccaagc tgtctaagct ggtgcgcgag
     1261 accgagcagg gcgccctgtt tagcgccgtg gcatgcgtga attccctggc catgctgacc
     1321 gcctccggca tcttcaactc tctgtatcct gccaccctga acttcatgaa gggcttccca
     1381 tttctgctgg gagcaggact gctgctgatc ccagccgtgc tgatcggcat gctggagaag
     1441 gccgatcctc acctggagtt ccagcagttt ccccagtccc cttaagaccc agctttcttg
     1501 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     1561 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     1621 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     1681 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     1741 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     1801 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1861 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1921 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     1981 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2041 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     2101 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     2161 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     2221 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2281 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     2341 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2401 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2461 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2521 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     2581 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     2641 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     2701 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2761 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2821 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2881 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2941 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3001 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3061 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3121 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3181 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3241 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3301 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3361 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3421 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3481 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3541 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     3601 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     3661 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     3721 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     3781 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     3841 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     3901 aaaacgacgg ccagtcttaa gctcgggccc
//
