LOCUS       sequence-408927-        8983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0760
FEATURES             Location/Qualifiers
     source          1..8983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1768..1978
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1790..1986
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1837..1972
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2018..2620
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2018..2037)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2514..2534
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2663..2780
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2918..4096
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3149..4087
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4044..4064
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4838..5426
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4891..4911)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5309..5320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5498..5731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5809..5930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5846..5865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5900..5919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5970..6105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6125..6144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6126..6144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6154..6171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6154..6170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6163..6185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6312..6767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6399..6418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6609..6630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6793..6897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6898..6966,6967..7758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7116..7135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7929..8517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8418..8437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8671..8688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8805..8826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8841..8846,8847..8864,8865..8871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8879..8895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8884..8906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8903..8919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8903..8919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8938..8958
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8940..8958
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     1741 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     1801 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1861 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1921 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1981 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     2041 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     2101 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     2161 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     2221 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     2281 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     2341 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     2401 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     2461 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     2521 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     2581 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     2641 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2701 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2761 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     2821 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     2881 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     2941 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     3001 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctscgcct
     3061 ttttcccgag ggtgggggag aacsgtatat aagtgcagta gtcgccgtga acgttctttt
     3121 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     3181 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     3241 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     3301 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     3361 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     3421 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     3481 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     3541 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     3601 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     3661 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3721 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3781 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3841 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3901 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3961 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     4021 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     4081 atttcaggtg tcgtgaggct agcaccgcca ccatgagcct tcgagactct agctacagca
     4141 tggcccccgg gccctgtgtg gtggcccagc tgccctctga agacatgggc cacttgacag
     4201 acccccagag cagagaccat ggcttcctgc gcaccaagat gaaggtgacc cttggggaca
     4261 gtcccagtgg agacctgttc acctgccgtg tctgccagaa ggccttcacc taccagcgca
     4321 tgctgaaccg ccacatgaag tgtcacaacg acgtcaagag gcacctctgc acgtactgcg
     4381 ggaagggctt caatgacacc ttcgacctca agagacacgt ccgaactcac actggcgtgc
     4441 ggccctacaa gtgcagcctg tgtgacaagg ccttcacgca gcgctgctct ctggagtctc
     4501 acctcaagaa gatccatggt gtgcagcaga agtacgcgta caaggagcgg cgggccaagc
     4561 tgtacgtgtg tgaggagtgc ggctgcacat ctgagagcca ggagggccac gtcctgcacc
     4621 tgaaggagca ccaccctgac agcccgctgc tgcgcaagac ctccaagaag gtggccgtgg
     4681 cactacagaa cactgtcact tccctgctgc agggcagccc ccacctgtag taatgaacta
     4741 gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgatt
     4801 ctggttgcta attctgtgca taacgcgtta agtcgacaat caacctctgg attacaaaat
     4861 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4921 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4981 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5041 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5101 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5161 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5221 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5281 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5341 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5401 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     5461 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     5521 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5701 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     5761 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     5821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     5941 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6061 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagytgc gcagcctgaa
     6301 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     6601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     6661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     6721 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     6781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     6841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     6901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     6961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     7441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     7501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     7561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     7621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     7681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     7741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     7801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     7861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     7981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     8641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     8701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     8761 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     8821 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     8881 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     8941 attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
