LOCUS       sequence-408929-        9637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0762
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        44..270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             271..451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    498..623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1116..1349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1534..1578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1809..2019
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1831..2027
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1878..2013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2059..2661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2059..2078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2555..2575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2704..2821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2959..4137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3190..4128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4085..4105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4148..4157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4152..4161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5533..6121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5586..5606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6004..6015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6193..6426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6504..6625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6541..6560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6595..6614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6665..6800
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6820..6839)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6821..6839)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6849..6866)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6849..6865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6858..6880)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7007..7462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7094..7113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7304..7325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7488..7592
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7593..7661,7662..8453)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7811..7830)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8624..9212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9113..9132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9366..9383
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9500..9521
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9536..9541,9542..9559,9560..9566)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9574..9590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9579..9601
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9598..9614
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9598..9614
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(9633..9637,1..16)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(9635..9637,1..16)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
       61 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
      121 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
      181 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
      241 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
      301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      421 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      481 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
      541 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
      601 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
      661 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
      721 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
      781 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
      841 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
      901 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
      961 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1021 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1081 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1141 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1201 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1261 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1321 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1381 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1441 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1501 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     1561 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     1621 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     1681 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     1741 ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga aagtccccag
     1801 gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca accatagtcc
     1861 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     1921 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1981 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctttctagag
     2041 gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     2101 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     2161 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     2221 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg
     2281 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     2341 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     2401 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     2461 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     2521 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     2581 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     2641 cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag tattcatcca
     2701 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2761 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2821 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg cctgcaggtg
     2881 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     2941 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     3001 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     3061 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     3121 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     3181 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     3241 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     3301 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     3361 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     3421 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     3481 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     3541 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     3601 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     3661 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     3721 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     3781 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3841 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3901 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3961 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     4021 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     4081 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaggc
     4141 tagcaccgcc accatggcgg ctgtcgtcct gcccccaact gccgcctctc agcgagaagg
     4201 acacacagag ggcggagagc tggttaatga gctcctgaaa agctggctaa agggcttggt
     4261 gacctttgag gatgtggccg tggagttcac ccaggaggag tgggcattgc tggaccctgc
     4321 ccaaaggaca ctgtacaggg acgtgatgct ggagaactgc aggaacctgg cctcactggg
     4381 gaaccaagtt gataaaccta ggctgatctc ccagctggag caagaagata aagtgatgac
     4441 agaagagaga ggaattctct caggtacctg tccagatgtg gagaatccat ttaaagccaa
     4501 agggttaact cctaagctgc atgtttttcg aaaagaacaa tctagaaata tgaaaatgga
     4561 gaggaatcat cttggagcaa cactcaacga atgtaatcag tgttttaaag tcttcagcac
     4621 aaaatcttcc cttacacggc acaggaagat tcatactgga gaaagaccct atggctgcag
     4681 tgaatgtggg aaatcctaca gcagtagatc ttaccttgct gttcataaga gaatccacaa
     4741 tggggagaaa ccctatgaat gcaatgactg tgggaaaacc ttcagcagca gatcttacct
     4801 tactgttcat aagagaatcc acaatgggga gaaaccctac gaatgcagtg actgtgggaa
     4861 aaccttcagc aattcctcat acctcagacc gcacttgaga attcacactg gagaaaaacc
     4921 gtacaaatgt aaccagtgtt ttcgtgagtt ccgcactcag tcaatcttca caaggcacaa
     4981 gagagttcat acgggggagg gtcattatgt atgtaatcag tgtggaaagg ctttcggcac
     5041 gaggtcatct ctttcttcgc actatagcat tcatacaggg gagtaccctt acgaatgcca
     5101 cgattgtggg agaaccttca ggaggaggtc gaatctgaca cagcacataa gaactcatac
     5161 tggagaaaaa ccctacacat gtaatgagtg tgggaaatcc tttaccaata gcttttctct
     5221 tacaattcac aggagaatac ataatggaga gaaatcctat gagtgcagtg attgtggaaa
     5281 atcctttaat gttctctcat ccgttaagaa acacatgaga actcacactg gaaaaaaacc
     5341 ctatgaatgt aattattgcg ggaaatcctt cacaagtaac tcctaccttt ctgtgcatac
     5401 gagaatgcat aataggcaaa tgtagtaatg aactagtccg taacttgaaa gtatttcgat
     5461 ttcttggctt tatatatctt gtggaaagga cgacgcgtta cattataccc tcgtaccacg
     5521 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5581 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5641 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5701 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5761 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5821 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5881 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     5941 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6001 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6061 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6121 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6181 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     6241 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6301 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6361 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6421 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6481 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6541 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6601 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6661 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6721 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6781 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6841 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6901 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6961 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     7021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7081 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7201 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7321 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7381 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7441 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7501 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7561 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7621 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7681 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7741 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7801 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7861 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7921 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7981 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8041 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8101 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8161 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8221 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8281 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8341 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8401 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8461 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8521 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8581 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8641 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8701 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8761 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8821 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8881 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8941 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9001 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9061 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9121 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9181 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9241 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9301 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9361 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9421 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     9481 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     9541 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9601 gaaacagcta tgaccatgat tacgccaagc gcgcaat
//
